protein

Recombinant S. plicatus Endo H Protein, CF

5224-GHB-050

50 ug

432 USD

5224-GHB-050

Recombinant S. plicatus Endo H Protein, CF

50 ug

The Recombinant S. plicatus Endo H Protein, CF from R&D Systems is derived from E. coli. The Recombinant S. plicatus Endo H Protein, CF has been validated for the following applications: Enzyme Activity.

Endo-beta-N-acetylglucosaminidase H/Endo H

Proteins and Enzymes

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl and EDTA.

Unconjugated

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

S. plicatus

29 kDa, reducing conditions

30 kDa

Measured by its ability to deglycosylate ribonuclease B under native conditions. The DC50 is 5 ng. The DC50 is defined as the amount of enzyme required to remove 50% of glycan on 1 ╡g of RNase B in 30 minutes at 37 ░C. Use of Recombinant S. plicatus Endo┐▀-N-acetylglucosaminidase H/Endo H in the deglycoslyation of other substrates may require alternative conditions for optimal performance.

<0.01 EU per 1 ╡g of the protein by the LAL method.

P04067

Enzyme Activity

E. coli -derived s. plicatus Endo-beta-N-acetylglucosaminidase H/Endo H protein Val47-Pro313 with an N-terminal Met and 6-His tag

E. coli

432 USD

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.