protein

Recombinant F. meningosepticum Endo F1 Protein, CF

5220-GH-050

50 ug

432 USD

5220-GH-050

Recombinant F. meningosepticum Endo F1 Protein, CF

50 ug

The Recombinant F. meningosepticum Endo F1 Protein, CF from R&D Systems is derived from E. coli. The Recombinant F. meningosepticum Endo F1 Protein, CF has been validated for the following applications: Enzyme Activity.

Endo-beta-N-acetylglucosaminidase F1/Endo F1

Proteins and Enzymes

Supplied as a 0.2 ╡m filtered solution in Tris and NaCl.

Unconjugated

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

F. meningosepticum

35 kDa, under reducing conditions

35 kDa

Measured by its ability to deglycosylate ribonuclease B under native conditions. The DC50 is 5 ng. The DC50 is defined as the amount of enzyme required to remove 50% of glycan on 1 ╡g of RNase B in 30 minutes at 37 ░C. Use of Recombinant F. meningosepticum Endo F1 in the delycoslyation of other substrates may require alternative conditions for optimal performance.

<1.0 EU per 1 ╡g of the protein by the LAL method.

P36911

Enzyme Activity

E. coli -derived f. meningosepticum Endo-beta-N-acetylglucosaminidase F1/Endo F1 protein Arg31-Trp339, with an N-terminal Met and 6-His tag Accession # P36911

E. coli

432 USD

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.