Recombinant Rat IL-10 Protein | R&D Systems 522-RLB-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Rat IL-10 Protein

catalog :

522-RLB-025

quantity :

25 ug

price :

453 USD

more info or order :

R&D Systems product webpage

citations: 13

Reference
Kim S, Son Y. Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia. Int J Mol Sci. 2021;22: pubmed publisher
Lively S, Schlichter L. Microglia Responses to Pro-inflammatory Stimuli (LPS, IFNγ+TNFα) and Reprogramming by Resolving Cytokines (IL-4, IL-10). Front Cell Neurosci. 2018;12:215 pubmed publisher
Erkens T, Goeminne N, Kegels A, Byloos M, Vinken P. Analytical performance of a commercial multiplex Luminex-based cytokine panel in the rat. J Pharmacol Toxicol Methods. 2018;91:43-49 pubmed publisher
Lim J, Chung E, Son Y. A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFNγ. Sci Rep. 2017;7:9417 pubmed publisher
Cambier L, de Couto G, Ibrahim A, Echavez A, Valle J, Liu W, et al. Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion. EMBO Mol Med. 2017;9:337-352 pubmed publisher
Stanisavljević S, Lukić J, Soković S, Mihajlovic S, Mostarica Stojkovic M, Miljkovic D, et al. Correlation of Gut Microbiota Composition with Resistance to Experimental Autoimmune Encephalomyelitis in Rats. Front Microbiol. 2016;7:2005 pubmed publisher
Ma H, Wang X, Wu M, Zhao X, Yuan X, Shi X. Interleukin-10 Contributes to Therapeutic Effect of Mesenchymal Stem Cells for Acute Liver Failure via Signal Transducer and Activator of Transcription 3 Signaling Pathway. Chin Med J (Engl). 2016;129:967-75 pubmed publisher
Siddiqui T, Lively S, Ferreira R, Wong R, Schlichter L. Expression and contributions of TRPM7 and KCa2.3/SK3 channels to the increased migration and invasion of microglia in anti-inflammatory activation states. PLoS ONE. 2014;9:e106087 pubmed publisher
Le Texier L, Durand J, Lavault A, Hulin P, Collin O, Le Bras Y, et al. LIMLE, a new molecule over-expressed following activation, is involved in the stimulatory properties of dendritic cells. PLoS ONE. 2014;9:e93894 pubmed publisher
Steelman A, Smith R, Welsh C, Li J. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis. J Biol Chem. 2013;288:23776-87 pubmed publisher
Ke Y, Jiang G, Sun D, Kaplan H, Shao H. Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis. Invest Ophthalmol Vis Sci. 2008;49:3999-4007 pubmed publisher
Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat. Crit Care Med. 2005;33:2317-22 pubmed
Lynch A, Walsh C, Delaney A, Nolan Y, Campbell V, Lynch M. Lipopolysaccharide-induced increase in signalling in hippocampus is abrogated by IL-10--a role for IL-1 beta?. J Neurochem. 2004;88:635-46 pubmed

image

image 1 :

Recombinant Rat IL-10 Protein Bioactivity

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

522-RLB

SKU :

522-RLB-025

product name :

Recombinant Rat IL-10 Protein

Description :

The Recombinant Rat IL-10 Protein from R&D Systems is derived from E. coli. The Recombinant Rat IL-10 Protein has been validated for the following applications: Bioactivity.

target :

IL-10

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Rat

observed molecular weight :

16 kDa, reducing conditions

theoretical molecular weight :

19 kDa (monomer)

details of functionality :

Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L. et al . (1991) J. Exp. Med. 173 :507. The ED50 for this effect is 0.12-0.72 ng/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P29456.1

applications :

Bioactivity

source :

E. coli-derived Recombinant Rat IL-10 Protein

USD :

453 USD

alt names :

CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage