Recombinant Mouse IL-34 Protein | R&D Systems 5195-ML-010 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse IL-34 Protein

catalog :

5195-ML-010

quantity :

10 ug

price :

432 USD

citations: 18

Reference
Goshi N, Kim H, Seker E. Primary Cortical Cell Tri-Culture-Based Screening of Neuroinflammatory Response in Toll-like Receptor Activation. Biomedicines. 2022;10: pubmed publisher
Schulz R, Korkut Demirba x15f M, Venturino A, Colombo G, Siegert S. Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responses. Nat Commun. 2022;13:4728 pubmed publisher
Tan W, Su P, Leff J, Gao X, Chen J, Guan A, et al. Distinct phases of adult microglia proliferation: a Myc-mediated early phase and a Tnfaip3-mediated late phase. Cell Discov. 2022;8:34 pubmed publisher
Poudel M, Kim G, Bhattarai P, Kim J, Choi H. Interleukin-34-CSF1R Signaling Axis Promotes Epithelial Cell Transformation and Breast Tumorigenesis. Int J Mol Sci. 2021;22: pubmed publisher
Obst J, Simon E, Martín Estebané M, Pipi E, Barkwill L, Gonzalez Rivera I, et al. Inhibition of IL-34 Unveils Tissue-Selectivity and Is Sufficient to Reduce Microglial Proliferation in a Model of Chronic Neurodegeneration. Front Immunol. 2020;11:579000 pubmed publisher
Guttenplan K, Weigel M, Adler D, Couthouis J, Liddelow S, Gitler A, et al. Knockout of reactive astrocyte activating factors slows disease progression in an ALS mouse model. Nat Commun. 2020;11:3753 pubmed publisher
Xu P, Shan C, Dunn T, Xie X, Xia H, Gao J, et al. Role of microglia in the dissemination of Zika virus from mother to fetal brain. PLoS Negl Trop Dis. 2020;14:e0008413 pubmed publisher
Goshi N, Morgan R, Lein P, Seker E. A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation. J Neuroinflammation. 2020;17:155 pubmed publisher
Schlepckow K, Monroe K, Kleinberger G, Cantuti Castelvetri L, Parhizkar S, Xia D, et al. Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region. EMBO Mol Med. 2020;12:e11227 pubmed publisher
Bernier L, Bohlen C, York E, Choi H, Kamyabi A, Dissing Olesen L, et al. Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia. Cell Rep. 2019;27:2895-2908.e4 pubmed publisher
Bohlen C, Bennett F, Tucker A, Collins H, Mulinyawe S, Barres B. Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures. Neuron. 2017;94:759-773.e8 pubmed publisher
Kuboyama K, Fujikawa A, Suzuki R, Tanga N, Noda M. Role of Chondroitin Sulfate (CS) Modification in the Regulation of Protein-tyrosine Phosphatase Receptor Type Z (PTPRZ) Activity: PLEIOTROPHIN-PTPRZ-A SIGNALING IS INVOLVED IN OLIGODENDROCYTE DIFFERENTIATION. J Biol Chem. 2016;291:18117-28 pubmed publisher
Jin S, Sonobe Y, Kawanokuchi J, Horiuchi H, Cheng Y, Wang Y, et al. Interleukin-34 restores blood-brain barrier integrity by upregulating tight junction proteins in endothelial cells. PLoS ONE. 2014;9:e115981 pubmed publisher
Boström E, Lundberg P. The newly discovered cytokine IL-34 is expressed in gingival fibroblasts, shows enhanced expression by pro-inflammatory cytokines, and stimulates osteoclast differentiation. PLoS ONE. 2013;8:e81665 pubmed publisher
Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, et al. CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34. J Leukoc Biol. 2014;95:19-31 pubmed publisher
Huynh D, Akcora D, Malaterre J, Chan C, Dai X, Bertoncello I, et al. CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response. PLoS ONE. 2013;8:e56951 pubmed publisher
Luo J, Elwood F, Britschgi M, Villeda S, Zhang H, Ding Z, et al. Colony-stimulating factor 1 receptor (CSF1R) signaling in injured neurons facilitates protection and survival. J Exp Med. 2013;210:157-72 pubmed publisher
Gow D, Garceau V, Pridans C, Gow A, Simpson K, Gunn Moore D, et al. Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34). Cytokine. 2013;61:630-8 pubmed publisher

product information

master code :

5195-ML

SKU :

5195-ML-010

product name :

Recombinant Mouse IL-34 Protein

unit size :

10 ug

description :

The Recombinant Mouse IL-34 Protein from R&D Systems is derived from NS0. The Recombinant Mouse IL-34 Protein has been validated for the following applications: Bioactivity.

target :

IL-34

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Mouse

observed molecular weight :

35-40 kDa, reducing conditions

theoretical molecular weight :

25.4 kDa

gene symbol :

Il34

details of functionality :

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al . (1990) J. Immunol. 145 :860. The ED50 for this effect is 10-40áng/mL.

endotoxin note :

<0.01 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q8R1R4.1

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived mouse IL-34 protein Asn21-Pro235, with a C-terminal 6-His tag

source short :

NS0

USD :

432 USD

alt names :

C16orf77, IL34, IL-34, interleukin 34

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.