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company name :
R&D Systems
product type :
protein
product name :
Recombinant Rat GDNF Protein, CF
catalog :
512-GF-010/CF
quantity :
10 ug (also 50 ug)
price :
351 USD
more info or order :
citations: 42
Reference
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VandenBosch L, Wohl S, Wilken M, Hooper M, Finkbeiner C, Cox K, et al. Developmental changes in the accessible chromatin, transcriptome and Ascl1-binding correlate with the loss in Müller Glial regenerative potential. Sci Rep. 2020;10:13615 pubmed publisher
Javadi A, Mokhtari S, Moraveji S, Sayahpour F, Farzaneh M, Gourabi H, et al. Short time exposure to low concentration of zinc oxide nanoparticles up-regulates self-renewal and spermatogenesis-related gene expression. Int J Biochem Cell Biol. 2020;127:105822 pubmed publisher
Kerman B, Genoud S, Kurt Vatandaslar B, Denli A, Georges Ghosh S, Xu X, et al. Motoneuron expression profiling identifies an association between an axonal splice variant of HDGF-related protein 3 and peripheral myelination. J Biol Chem. 2020;295:12233-12246 pubmed publisher
Hartung J, Gold M. GCaMP as an indirect measure of electrical activity in rat trigeminal ganglion neurons. Cell Calcium. 2020;89:102225 pubmed publisher
Kuroda S, Miyagawa Y, Sato Y, Yamamoto M, Adachi K, Kinoh H, et al. Protocol Optimization for the Production of the Non-Cytotoxic JΔNI5 HSV Vector Deficient in Expression of Immediately Early Genes. Mol Ther Methods Clin Dev. 2020;17:612-621 pubmed publisher
Fu K, Tian S, Tan H, Wang C, Wang H, Wang M, et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol. 2019;17:39 pubmed publisher
Wu C, Watts M, Rubin L. MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis. Cell Rep. 2019;26:1143-1156.e5 pubmed publisher
Bai Y, Zhu C, Feng M, Wei H, Li L, Tian X, et al. Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells. Stem Cell Res Ther. 2018;9:200 pubmed publisher
Gibbs K, Kalmar B, Rhymes E, Fellows A, Ahmed M, Whiting P, et al. Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS. Cell Death Dis. 2018;9:596 pubmed publisher
Cabrera J, Charron A, Leib D. Neuronal Subtype Determines Herpes Simplex Virus 1 Latency-Associated-Transcript Promoter Activity during Latency. J Virol. 2018;92: pubmed publisher
Aoki N, Mochizuki K, Matsui Y. DNA methylation of the Fthl17 5'-upstream region regulates differential Fthl17 expression in lung cancer cells and germline stem cells. PLoS ONE. 2017;12:e0172219 pubmed publisher
Ishikura Y, Yabuta Y, Ohta H, Hayashi K, Nakamura T, Okamoto I, et al. In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells. Cell Rep. 2016;17:2789-2804 pubmed publisher
Vilmont V, Cadot B, Ouanounou G, Gomes E. A system for studying mechanisms of neuromuscular junction development and maintenance. Development. 2016;143:2464-77 pubmed publisher
Suzuki A, Hirasaki M, Hishida T, Wu J, Okamura D, Ueda A, et al. Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells. Nat Commun. 2016;7:11056 pubmed publisher
Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, et al. Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development. PLoS ONE. 2015;10:e0129944 pubmed publisher
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Fonseca Pereira D, Arroz Madeira S, Rodrigues Campos M, Barbosa I, Domingues R, Bento T, et al. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function. Nature. 2014;514:98-101 pubmed publisher
Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer Dene B, et al. Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors. EMBO J. 2014;33:1582-98 pubmed publisher
Lim J, Shim M, Lee J, Lee D. Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells. PLoS ONE. 2014;9:e90454 pubmed publisher
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Dovere L, Fera S, Grasso M, Lamberti D, Gargioli C, Muciaccia B, et al. The niche-derived glial cell line-derived neurotrophic factor (GDNF) induces migration of mouse spermatogonial stem/progenitor cells. PLoS ONE. 2013;8:e59431 pubmed publisher
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Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, et al. Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM). Mol Cell Neurosci. 2013;54:30-43 pubmed publisher
Touchard E, Heiduschka P, Berdugo M, Kowalczuk L, Bigey P, Chahory S, et al. Non-viral gene therapy for GDNF production in RCS rat: the crucial role of the plasmid dose. Gene Ther. 2012;19:886-98 pubmed publisher
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Kanatsu Shinohara M, Muneto T, Lee J, Takenaka M, Chuma S, Nakatsuji N, et al. Long-term culture of male germline stem cells from hamster testes. Biol Reprod. 2008;78:611-7 pubmed
He Z, Jiang J, Kokkinaki M, Golestaneh N, Hofmann M, Dym M. Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation. Stem Cells. 2008;26:266-78 pubmed
Garbayo E, Ansorena E, Lanciego J, Aymerich M, Blanco Prieto M. Purification of bioactive glycosylated recombinant glial cell line-derived neurotrophic factor. Int J Pharm. 2007;344:9-15 pubmed
Hamra F, Chapman K, Nguyen D, Garbers D. Identification of neuregulin as a factor required for formation of aligned spermatogonia. J Biol Chem. 2007;282:721-30 pubmed
Murata T, Tsuboi M, Hikita K, Kaneda N. Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D. J Biol Chem. 2006;281:22503-16 pubmed
Obata K, Katsura H, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, et al. TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury. J Clin Invest. 2005;115:2393-401 pubmed
Carmillo P, Dagø L, Day E, Worley D, Rossomando A, Walus L, et al. Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin. Biochemistry. 2005;44:2545-54 pubmed
image
image 1 :
R&D Systems 512-GF-010/CF image 1
Recombinant Rat GDNF Protein Bioactivity
product information
brand :
R&D Systems, a Bio-Techne Brand
catalog number base :
512-GF/CF
SKU :
512-GF-010/CF
product name :
Recombinant Rat GDNF Protein, CF
Description :
The Recombinant Rat GDNF Protein, CF from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Rat GDNF Protein, CF has been validated for the following applications: Bioactivity, Binding Activity.
target :
GDNF
category :
Proteins and Enzymes
unit size :
10 ug (also 50 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Rat
theoretical molecular weight :
15 kDa (monomer)
gene symbol :
Gdnf
details of functionality :
Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Rat GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 560-GR ). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 560-GR ) at 1 µg/mL binds Recombinant Rat GDNF with an apparent Kd 4 nM.
endotoxin note :
<0.01 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
Q07731
applications :
Bioactivity, Binding Activity
source :
Sf 21 (baculovirus)-derived Recombinant Rat GDNF Protein
USD :
351 USD
alt names :
Astrocyte-derived trophic factor, ATF, ATF1, ATF2, glial cell derived neurotrophic factor, glial cell line derived neurotrophic factor, glial cell line-derived neurotrophic factor, HFB1-GDNF, HGDNF, HSCR3
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.