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company name :
R&D Systems
product type :
protein
product name :
Recombinant Rat TNF-alpha Protein, CF
catalog :
510-RT-010/CF
quantity :
10 ug (also 50 ug)
price :
259 USD
more info or order :
citations: 66
Reference
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Zhang C, Yan J, Xiao Y, Shen Y, Wang J, Ge W, et al. Inhibition of Autophagic Degradation Process Contributes to Claudin-2 Expression Increase and Epithelial Tight Junction Dysfunction in TNF-α Treated Cell Monolayers. Int J Mol Sci. 2017;18: pubmed publisher
Yuan J, Liu W, Zhu H, Chen Y, Zhang X, Li L, et al. Curcumin inhibits glial scar formation by suppressing astrocyte-induced inflammation and fibrosis in vitro and in vivo. Brain Res. 2017;1655:90-103 pubmed publisher
Zhu Q, Huang J, Wang S, Qin Z, Lin F. Cobrotoxin extracted from Naja atra venom relieves arthritis symptoms through anti-inflammation and immunosuppression effects in rat arthritis model. J Ethnopharmacol. 2016;194:1087-1095 pubmed publisher
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Lazzaro M, Bettegazzi B, Barbariga M, Codazzi F, Zacchetti D, Alessio M. Ceruloplasmin potentiates nitric oxide synthase activity and cytokine secretion in activated microglia. J Neuroinflammation. 2014;11:164 pubmed publisher
Maidhof R, Jacobsen T, Papatheodorou A, Chahine N. Inflammation induces irreversible biophysical changes in isolated nucleus pulposus cells. PLoS ONE. 2014;9:e99621 pubmed publisher
Lindblom R, Ström M, Heinig M, Al Nimer F, Aeinehband S, Berg A, et al. Unbiased expression mapping identifies a link between the complement and cholinergic systems in the rat central nervous system. J Immunol. 2014;192:1138-53 pubmed publisher
Lim S, Park E, You B, Jung Y, Park A, Park S, et al. Neuronal synapse formation induced by microglia and interleukin 10. PLoS ONE. 2013;8:e81218 pubmed publisher
Macco R, Pelizzoni I, Consonni A, Vitali I, Giacalone G, Martinelli Boneschi F, et al. Astrocytes acquire resistance to iron-dependent oxidative stress upon proinflammatory activation. J Neuroinflammation. 2013;10:130 pubmed publisher
Steelman A, Smith R, Welsh C, Li J. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis. J Biol Chem. 2013;288:23776-87 pubmed publisher
Weaver J, Taylor Fishwick D. Regulation of NOX-1 expression in beta cells: a positive feedback loop involving the Src-kinase signaling pathway. Mol Cell Endocrinol. 2013;369:35-41 pubmed publisher
Si J, Ge Y, Zhuang S, Wang L, Chen S, Gong R. Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms. Kidney Int. 2013;83:635-46 pubmed publisher
Huie J, Baumbauer K, Lee K, Bresnahan J, Beattie M, Ferguson A, et al. Glial tumor necrosis factor alpha (TNF?) generates metaplastic inhibition of spinal learning. PLoS ONE. 2012;7:e39751 pubmed publisher
Si J, Ge Y, Zhuang S, Gong R. Inhibiting nonmuscle myosin II impedes inflammatory infiltration and ameliorates progressive renal disease. Lab Invest. 2010;90:448-58 pubmed publisher
Schwarznau A, Hanson M, Sperger J, Schram B, Danobeitia J, Greenwood K, et al. IL-1beta receptor blockade protects islets against pro-inflammatory cytokine induced necrosis and apoptosis. J Cell Physiol. 2009;220:341-7 pubmed publisher
Kato K, Kikuchi S, Shubayev V, Myers R. Distribution and tumor necrosis factor-alpha isoform binding specificity of locally administered etanercept into injured and uninjured rat sciatic nerve. Neuroscience. 2009;160:492-500 pubmed publisher
Ferguson A, Christensen R, Gensel J, Miller B, Sun F, Beattie E, et al. Cell death after spinal cord injury is exacerbated by rapid TNF alpha-induced trafficking of GluR2-lacking AMPARs to the plasma membrane. J Neurosci. 2008;28:11391-400 pubmed publisher
Wei F, Guo W, Zou S, Ren K, Dubner R. Supraspinal glial-neuronal interactions contribute to descending pain facilitation. J Neurosci. 2008;28:10482-95 pubmed publisher
Churchill L, Rector D, Yasuda K, Fix C, Rojas M, Yasuda T, et al. Tumor necrosis factor alpha: activity dependent expression and promotion of cortical column sleep in rats. Neuroscience. 2008;156:71-80 pubmed publisher
Berger S, Savitz S, Nijhawan S, Singh M, David J, Rosenbaum P, et al. Deleterious role of TNF-alpha in retinal ischemia-reperfusion injury. Invest Ophthalmol Vis Sci. 2008;49:3605-10 pubmed publisher
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Khan A, Diogenes A, Jeske N, Henry M, Akopian A, Hargreaves K. Tumor necrosis factor alpha enhances the sensitivity of rat trigeminal neurons to capsaicin. Neuroscience. 2008;155:503-9 pubmed publisher
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product information
brand :
R&D Systems
master code :
510-RT/CF
SKU :
510-RT-010/CF
product name :
Recombinant Rat TNF-alpha Protein, CF
description :
The Recombinant Rat TNF-alpha Protein, CF from R&D Systems is derived from E. coli. The Recombinant Rat TNF-alpha Protein, CF has been validated for the following applications: Bioactivity.
target :
TNF-alpha
category :
Proteins and Enzymes
sizes available :
10 ug (also 50 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS and DTT.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Rat
theoretical molecular weight :
17 kDa
gene symbol :
Tnf
details of functionality :
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 4-20 pg/mL.
endotoxin note :
<0.1 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P16599
applications :
Bioactivity
source :
E. coli-derived Recombinant Rat TNF-alpha Protein, CF
USD :
259
USD 2023 :
259 USD
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.