product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Wnt-3a (High Purity) Protein
catalog :
5036-WNP-010
quantity :
10 ug
price :
965 USD
more info or order :
R&D Systems product webpage
citations: 4
Reference
Krumm J, Sekine K, Samaras P, Brazovskaja A, Breunig M, Yasui R, et al. High temporal resolution proteome and phosphoproteome profiling of stem cell-derived hepatocyte development. Cell Rep. 2022;38:110604 pubmed publisher
Carthy J, Engström U, Heldin C, Moustakas A. Commercially Available Preparations of Recombinant Wnt3a Contain Non-Wnt Related Activities Which May Activate TGF-β Signaling. J Cell Biochem. 2016;117:938-45 pubmed publisher
Bilir B, Kucuk O, Moreno C. Wnt signaling blockage inhibits cell proliferation and migration, and induces apoptosis in triple-negative breast cancer cells. J Transl Med. 2013;11:280 pubmed publisher
Bourhis E, Tam C, Franke Y, Bazan J, Ernst J, Hwang J, et al. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J Biol Chem. 2010;285:9172-9 pubmed publisher
image
image 1 :
R&D Systems 5036-WNP-010 image 1
Recombinant Human Wnt-3a (High Purity) Protein Bioactivity
product information
brand :
R&D Systems, a Bio-Techne Brand
catalog number base :
5036-WNP
SKU :
5036-WNP-010
product name :
Recombinant Human Wnt-3a (High Purity) Protein
Description :
The Recombinant Human Wnt-3a (High Purity) Protein from R&D Systems is derived from CHO. The Recombinant Human Wnt-3a (High Purity) Protein has been validated for the following applications: Bioactivity.
target :
Wnt-3a
category :
Proteins and Enzymes
unit size :
10 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE with silver staining
species :
Human
observed molecular weight :
40 kDa, reducing conditions
theoretical molecular weight :
37.4 kDa
gene symbol :
WNT3A
details of functionality :
Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 1.5-7.5 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
NP_149122
applications :
Bioactivity
source :
CHO-derived Recombinant Human Wnt-3a (High Purity) Protein
USD :
965 USD
alt names :
MGC119418, MGC119419, MGC119420, protein Wnt-3a, wingless-type MMTV integration site family, member 3A, Wnt3a
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.