Recombinant Human Wnt-3a (High Purity) Protein, CF | R&D Systems 5036-WNP-010/CF product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Wnt-3a (High Purity) Protein, CF

catalog :

5036-WNP-010/CF

quantity :

10 ug

price :

994 USD

citations: 4

Reference
Krumm J, Sekine K, Samaras P, Brazovskaja A, Breunig M, Yasui R, et al. High temporal resolution proteome and phosphoproteome profiling of stem cell-derived hepatocyte development. Cell Rep. 2022;38:110604 pubmed publisher
Carthy J, EngstrÃ¶m U, Heldin C, Moustakas A. Commercially Available Preparations of Recombinant Wnt3a Contain Non-Wnt Related Activities Which May Activate TGF-Î² Signaling. J Cell Biochem. 2016;117:938-45 pubmed publisher
Bilir B, Kucuk O, Moreno C. Wnt signaling blockage inhibits cell proliferation and migration, and induces apoptosis in triple-negative breast cancer cells. J Transl Med. 2013;11:280 pubmed publisher
Bourhis E, Tam C, Franke Y, Bazan J, Ernst J, Hwang J, et al. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J Biol Chem. 2010;285:9172-9 pubmed publisher

product information

master code :

5036-WNP/CF

SKU :

5036-WNP-010/CF

product name :

Recombinant Human Wnt-3a (High Purity) Protein, CF

unit size :

10 ug

description :

The Recombinant Human Wnt-3a (High Purity) Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Wnt-3a (High Purity) Protein, CF has been validated for the following applications: Bioactivity.

target :

Wnt-3a

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS, NaCl, EDTA and CHAPS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE with silver staining

species :

Human

observed molecular weight :

40 kDa, reducing conditions

theoretical molecular weight :

37.4 kDa

gene symbol :

WNT3A

details of functionality :

Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 1.5-7.5 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

top caption :

Recombinant Human Wnt-3a (High Purity) Protein Bioactivity

accessionNumbers :

NP_149122

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein Ser19-Lys352

source short :

CHO

USD :

994 USD

alt names :

MGC119418, MGC119419, MGC119420, protein Wnt-3a, wingless-type MMTV integration site family, member 3A, Wnt3a

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.