Recombinant Human Wnt-3a GMP Protein, CF | R&D Systems 5036-GMP-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Wnt-3a GMP Protein, CF

catalog :

5036-GMP-010

quantity :

10 ug (also 1 mg, 50 ug)

price :

593 USD

more info or order :

R&D Systems product webpage

citations: 1

Reference
Cho S. APC downregulated 1 inhibits breast cancer cell invasion by inhibiting the canonical WNT signaling pathway. Oncol Lett. 2017;14:4845-4852 pubmed publisher

image

image 1 :

Recombinant Human Wnt-3a GMP Protein Bioactivity

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

5036-GMP

SKU :

5036-GMP-010

product name :

Recombinant Human Wnt-3a GMP Protein, CF

Description :

The Recombinant Human Wnt-3a GMP Protein, CF from R&D Systems is derived from CHO. The Recombinant Human Wnt-3a GMP Protein, CF has been validated for the following applications: Bioactivity.

target :

Wnt-3a

category :

Proteins and Enzymes

unit size :

10 ug (also 1 mg, 50 ug)

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS.

conjugate :

Unconjugated

purity :

>75%, by SDS-PAGE with silver staining, under reducing conditions.

species :

Human

observed molecular weight :

40 kDa, reducing conditions

theoretical molecular weight :

37.4 kDa

gene symbol :

WNT3A

details of functionality :

Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 5-25 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

NP_149122.1

applications :

Bioactivity

source :

CHO-derived Recombinant Human Wnt-3a GMP Protein

USD :

593 USD

alt names :

MGC119418, MGC119419, MGC119420, protein Wnt-3a, wingless-type MMTV integration site family, member 3A, Wnt3a

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at <= -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage