Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein | R&D Systems 499-ML-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein

catalog :

499-ML-025

quantity :

25 ug

price :

451 USD

more info or order :

R&D Systems product webpage

citations: 10

Reference
Mondal S, Kundu M, Jana M, Roy A, Rangasamy S, Modi K, et al. IL-12 p40 monomer is different from other IL-12 family members to selectively inhibit IL-12Rβ1 internalization and suppress EAE. Proc Natl Acad Sci U S A. 2020;117:21557-21567 pubmed publisher
Marks E, Naudin C, Nolan G, Goggins B, Burns G, Mateer S, et al. Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation. Mucosal Immunol. 2017;10:1224-1236 pubmed publisher
Daria A, Colombo A, Llovera G, Hampel H, Willem M, Liesz A, et al. Young microglia restore amyloid plaque clearance of aged microglia. EMBO J. 2017;36:583-603 pubmed publisher
Abdi K, Singh N, Spooner E, Kessler B, Radaev S, Lantz L, et al. Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly. J Immunol. 2014;192:6028-36 pubmed publisher
Jana M, Mondal S, Jana A, Pahan K. Interleukin-12 (IL-12), but not IL-23, induces the expression of IL-7 in microglia and macrophages: implications for multiple sclerosis. Immunology. 2014;141:549-63 pubmed publisher
Brahmachari S, Pahan K. Suppression of regulatory T cells by IL-12p40 homodimer via nitric oxide. J Immunol. 2009;183:2045-58 pubmed publisher
An H, Jeong H, Um J, Kim H, Hong S. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. J Ethnopharmacol. 2006;106:418-24 pubmed
Bengtsson A, Ryan E, Giordano D, Magaletti D, Clark E. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood. 2004;104:1404-10 pubmed
Huaux F, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier J, et al. A profibrotic function of IL-12p40 in experimental pulmonary fibrosis. J Immunol. 2002;169:2653-61 pubmed
Lukacs N, Berlin A, Schols D, Skerlj R, Bridger G. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Am J Pathol. 2002;160:1353-60 pubmed

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

499-ML

SKU :

499-ML-025

product name :

Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein

Description :

The Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein has been validated for the following applications: Bioactivity.

target :

IL-12/IL-23 p40

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Mouse

theoretical molecular weight :

35.8 kDa (monomer)

gene symbol :

Il12b

details of functionality :

Measured by its ability to inhibit the IL-12 dependent proliferation of mouse splenocytes activated with PHA and IL-2. The ED50 for this effect is 1-3 ng/mL in the presence of 0.3 ng/mL of mouse IL-12.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P43432.1

applications :

Bioactivity

source :

Sf 21 (baculovirus)-derived Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein

USD :

451 USD

alt names :

CLMF, CLMF p40, CLMF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL12 p40, IL-12 subunit p40, IL12B, IL-12B, IL-12BNK cell stimulatory factor chain 2, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40), interleukin-12 beta chain, interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NKSF, NKSF2, NKSF2IL12, subunit p40

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage