product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse Thrombopoietin Protein
catalog :
488-TO-005
quantity :
5 ug
price :
356 USD
more info or order :
citations: 40
Reference
Naidu S, Capitano M, Ropa J, Cooper S, Huang X, Broxmeyer H. Chromatin remodeling subunit BRM and valine regulate hematopoietic stem/progenitor cell function and self-renewal via intrinsic and extrinsic effects. Leukemia. 2022;36:821-833 pubmed publisher
Bogert N, Furkel J, Din S, Braren I, Eckstein V, Muller J, et al. A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus. Sci Rep. 2020;10:13740 pubmed publisher
Garcia Perez L, van Eggermond M, Van Roon L, Vloemans S, Cordes M, Schambach A, et al. Successful Preclinical Development of Gene Therapy for Recombinase-Activating Gene-1-Deficient SCID. Mol Ther Methods Clin Dev. 2020;17:666-682 pubmed publisher
Lee Y, Kwak M, Moon S, Choi Y, Baek J, Park S, et al. Regulation of bone metabolism by megakaryocytes in a paracrine manner. Sci Rep. 2020;10:2277 pubmed publisher
Bertho J, Kereselidze D, Manens L, Culeux C, Magneron V, Surette J, et al. Hto, Tritiated Amino Acid Exposure and External Exposure Induce Differential Effects on Hematopoiesis and Iron Metabolism. Sci Rep. 2019;9:19919 pubmed publisher
Wiley C, Liu S, Limbad C, Zawadzka A, Beck J, Demaria M, et al. SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells. Cell Rep. 2019;28:3329-3337.e5 pubmed publisher
Capitano M, Mor Vaknin N, Saha A, Cooper S, Legendre M, Guo H, et al. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling. J Clin Invest. 2019;129:2555-2570 pubmed publisher
Tasian S, Casas J, Posocco D, Gandre Babbe S, Gagne A, Liang G, et al. Mutation-specific signaling profiles and kinase inhibitor sensitivities of juvenile myelomonocytic leukemia revealed by induced pluripotent stem cells. Leukemia. 2019;33:181-190 pubmed publisher
Wünsche P, Eckert E, Holland Letz T, Paruzynski A, Hotz Wagenblatt A, Fronza R, et al. Mapping Active Gene-Regulatory Regions in Human Repopulating Long-Term HSCs. Cell Stem Cell. 2018;23:132-146.e9 pubmed publisher
Sasine J, Himburg H, Termini C, Roos M, Tran E, Zhao L, et al. Wild-type Kras expands and exhausts hematopoietic stem cells. JCI Insight. 2018;3: pubmed publisher
Fang H, El Farran C, Xing Q, Zhang L, Li H, Lim B, et al. Global H3.3 dynamic deposition defines its bimodal role in cell fate transition. Nat Commun. 2018;9:1537 pubmed publisher
Rein L, Wisler J, Kim J, Theriot B, Huang L, Price T, et al. β-Arrestin2 mediates progression of murine primary myelofibrosis. JCI Insight. 2017;2: pubmed publisher
Skamagki M, Correia C, Yeung P, Baslan T, Beck S, Zhang C, et al. ZSCAN10 expression corrects the genomic instability of iPSCs from aged donors. Nat Cell Biol. 2017;19:1037-1048 pubmed publisher
Huang J, Khan A, Au B, Barber D, Lopez Vasquez L, Prokopishyn N, et al. Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34+ Cells for Correction of Fabry Disease. Mol Ther Methods Clin Dev. 2017;5:241-258 pubmed publisher
Xu C, Lee T, Sakurai N, Krchma K, Liu F, Li D, et al. ETV2/ER71 regulates hematopoietic regeneration by promoting hematopoietic stem cell proliferation. J Exp Med. 2017;214:1643-1653 pubmed publisher
Guo B, Huang X, Cooper S, Broxmeyer H. Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment. Nat Med. 2017;23:424-428 pubmed publisher
CASTILLO G, Nishimoto Ashfield A, Jones C, Kabirov K, Zakharov A, Lyubimov A. Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury. PLoS ONE. 2017;12:e0171703 pubmed publisher
Cheng H, Ang H, A El Farran C, Li P, Fang H, Liu T, et al. Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors. Nat Commun. 2016;7:13396 pubmed publisher
Lee S, Dvinge H, Kim E, Cho H, Micol J, Chung Y, et al. Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins. Nat Med. 2016;22:672-8 pubmed publisher
Wang F, Gao J, Malisani A, Xi X, Han W, Wan X. Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis. BMC Biotechnol. 2015;15:105 pubmed publisher
Schumacher A, Denecke B, Braunschweig T, Stahlschmidt J, Ziegler S, Brandenburg L, et al. Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy. J Hematol Oncol. 2015;8:64 pubmed publisher
Kim P, Nakano H, Das P, Chen M, Rowe R, Chou S, et al. Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence. J Exp Med. 2015;212:633-48 pubmed publisher
Begonja A, Pluthero F, Suphamungmee W, Giannini S, Christensen H, Leung R, et al. FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets. Blood. 2015;126:80-8 pubmed publisher
Bender M, Giannini S, Grozovsky R, Jönsson T, Christensen H, Pluthero F, et al. Dynamin 2-dependent endocytosis is required for normal megakaryocyte development in mice. Blood. 2015;125:1014-24 pubmed publisher
Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, et al. CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor. Stem Cells. 2015;33:976-87 pubmed publisher
Mayle A, Yang L, Rodriguez B, Zhou T, Chang E, Curry C, et al. Dnmt3a loss predisposes murine hematopoietic stem cells to malignant transformation. Blood. 2015;125:629-38 pubmed
Fonseca Pereira D, Arroz Madeira S, Rodrigues Campos M, Barbosa I, Domingues R, Bento T, et al. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function. Nature. 2014;514:98-101 pubmed publisher
Weston W, Zayas J, Perez R, George J, Jurecic R. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets. Sci Rep. 2014;4:5199 pubmed publisher
Nakahara F, Kitaura J, Uchida T, Nishida C, Togami K, Inoue D, et al. Hes1 promotes blast crisis in chronic myelogenous leukemia through MMP-9 upregulation in leukemic cells. Blood. 2014;123:3932-42 pubmed publisher
Hannibal R, Chuong E, Rivera Mulia J, Gilbert D, Valouev A, Baker J. Copy number variation is a fundamental aspect of the placental genome. PLoS Genet. 2014;10:e1004290 pubmed publisher
Park S, Umemoto T, Saito Adachi M, Shiratsuchi Y, Yamato M, Nakai K. Computational promoter modeling identifies the modes of transcriptional regulation in hematopoietic stem cells. PLoS ONE. 2014;9:e93853 pubmed publisher
Nobuhisa I, Osawa M, Uemura M, Kishikawa Y, Anani M, Harada K, et al. Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters. Mol Cell Biol. 2014;34:1976-90 pubmed publisher
Zimdahl B, Ito T, Blevins A, Bajaj J, Konuma T, Weeks J, et al. Lis1 regulates asymmetric division in hematopoietic stem cells and in leukemia. Nat Genet. 2014;46:245-52 pubmed publisher
Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, et al. Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports. 2013;1:545-59 pubmed publisher
Pereira C, Chang B, Qiu J, Niu X, Papatsenko D, Hendry C, et al. Induction of a hemogenic program in mouse fibroblasts. Cell Stem Cell. 2013;13:205-18 pubmed publisher
Cook B, Liu S, Evans T. Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment. Blood. 2011;117:6489-97 pubmed publisher
Zhang P, Nelson S, Bagby G, Siggins R, Shellito J, Welsh D. The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice. Stem Cells. 2008;26:1778-86 pubmed publisher
Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish H, et al. Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cells. Stem Cells. 2008;26:1628-35 pubmed publisher
Yilmaz O, Kiel M, Morrison S. SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity. Blood. 2006;107:924-30 pubmed
Van der Meeren A, Mouthon M, Vandamme M, Squiban C, Aigueperse J. Combinations of cytokines promote survival of mice and limit acute radiation damage in concert with amelioration of vascular damage. Radiat Res. 2004;161:549-59 pubmed
image
image 1 :
R&D Systems 488-TO-005 image 1
Recombinant Mouse Thrombopoietin Protein Bioactivity
product information
brand :
R&D Systems
master code :
488-TO
SKU :
488-TO-005
product name :
Recombinant Mouse Thrombopoietin Protein
unit size :
5 ug
description :
The Recombinant Mouse Thrombopoietin Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse Thrombopoietin Protein, CF has been validated for the following applications: Bioactivity.
target :
Thrombopoietin/Tpo
category :
Proteins and Enzymes
alt names :
Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
all available conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Mouse
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
observed molecular weight :
80-90 kDa, reducing conditions
theoretical molecular weight :
35 kDa
gene symbol :
Thpo
details of functionality :
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al . (1988) Br. J. Haematol. 69 :359. The ED50 for this effect is 0.4-2.4 ng/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P40226
applications :
Bioactivity
source :
NS0-derived Recombinant Mouse Thrombopoietin Protein
USD :
329
Jul-22 :
356 USD
sizes available :
5 ug (also 25 ug, 200 ug)
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.