Recombinant Mouse CD28 Fc Chimera Protein, CF | R&D Systems 483-CD-200/CF product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse CD28 Fc Chimera Protein, CF

catalog :

483-CD-200/CF

quantity :

200 ug

price :

470 USD

more info or order :

R&D Systems product webpage

citations: 7

Reference
Hanson A, Elpek K, Duong E, Shallberg L, Fan M, Johnson C, et al. ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models. PLoS ONE. 2020;15:e0239595 pubmed publisher
Carmi Y, Spitzer M, Linde I, Burt B, Prestwood T, Perlman N, et al. Allogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity. Nature. 2015;521:99-104 pubmed publisher
Kowalczyk A, D Souza C, Zhang L. Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3. Eur J Immunol. 2014;44:1143-55 pubmed publisher
Corse E, Gottschalk R, Park J, Sepulveda M, Loke P, Sullivan T, et al. Cutting edge: chronic inflammatory liver disease in mice expressing a CD28-specific ligand. J Immunol. 2013;190:526-30 pubmed publisher
Yamashita T, Ohneda K, Nagano M, Miyoshi C, Kaneko N, Miwa Y, et al. Hypoxia-inducible transcription factor-2alpha in endothelial cells regulates tumor neovascularization through activation of ephrin A1. J Biol Chem. 2008;283:18926-36 pubmed publisher
Kin N, Sanders V. CD86 regulates IgG1 production via a CD19-dependent mechanism. J Immunol. 2007;179:1516-23 pubmed
Arnett H, Escobar S, Gonzalez Suarez E, Budelsky A, Steffen L, Boiani N, et al. BTNL2, a butyrophilin/B7-like molecule, is a negative costimulatory molecule modulated in intestinal inflammation. J Immunol. 2007;178:1523-33 pubmed

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

483-CD/CF

SKU :

483-CD-200/CF

product name :

Recombinant Mouse CD28 Fc Chimera Protein, CF

Description :

The Recombinant Mouse CD28 Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse CD28 Fc Chimera Protein, CF has been validated for the following applications: Bioactivity.

target :

CD28

category :

Proteins and Enzymes

unit size :

200 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Mouse

observed molecular weight :

60 kDa, reducing conditions

theoretical molecular weight :

52 kDa (monomer)

gene symbol :

Cd28

details of functionality :

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Freeman, G.J. et al . (1993) Science 262 :909. The ED50 for this effect is 0.3-1.2 µg/mL when stimulated with 1 µg/mL recombinant human B7-1 Fc Chimera in the presence of PHA.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P31041

applications :

Bioactivity

source :

NS0-derived Recombinant Mouse CD28 Fc Chimera Protein

USD :

470 USD

alt names :

CD28, CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage