protein

Recombinant Human GPR114 Protein, CF

4747-GP-050

50 ug

432 USD

4747-GP-050

Recombinant Human GPR114 Protein, CF

50 ug

The Recombinant Human GPR114 Protein, CF from R&D Systems is derived from CHO. The Recombinant Human GPR114 Protein, CF has been validated for the following applications: Bioactivity.

GPR114

Proteins and Enzymes

Lyophilized from a 0.2 ╡m filtered solution in PBS.

Unconjugated

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Human

40-55 kDa, reducing conditions

19.4 kDa

ADGRG5

Measured by the ability of the immobilized protein to enhance the adhesion of T98G human glioblastoma cells to human Fibronectin. When 4 x 104 cells/well are added to plates coated with human Fibronectin (0.1 ╡g/mL, Catalog # 1918-FN) and rhGPR114 (10 ╡g/well, 100 ╡L/well) for 60 minutes at 37░ C, there is a 2-4 fold increase in adhesion to human fibronectin induced by the presence of rhGPR114. Optimal dilutions should be determined by each laboratory for each application.

<0.01 EU per 1 ╡g of the protein by the LAL method.

Q8IZF4

Bioactivity

Chinese Hamster Ovary cell line, CHO-derived human GPR114 protein Glu22-Gly184, with a C-terminal 6-His tag

CHO

432 USD

G protein-coupled receptor 114, G protein-coupled receptor PGR27, G-protein coupled receptor PGR27, PGR27, PGR27G-protein coupled receptor 114, probable G-protein coupled receptor 114

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.