Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein | R&D Systems 472-FF-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein

catalog :

472-FF-025

quantity :

25 ug

price :

432 USD

more info or order :

R&D Systems product webpage

citations: 14

Reference
Sokolova D, Ghansah S, Puletti F, Georgiades T, De Schepper S, Zheng Y, et al. Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models. bioRxiv. 2024;: pubmed publisher
Demetriou A, Lindqvist B, Ali H, Shamekh M, Maioli S, Inzunza J, et al. ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer's disease. bioRxiv. 2024;: pubmed publisher
De Schepper S, Ge J, Crowley G, Ferreira L, Garceau D, Toomey C, et al. Perivascular cells induce microglial phagocytic states and synaptic engulfment via SPP1 in mouse models of Alzheimer's disease. Nat Neurosci. 2023;26:406-415 pubmed publisher
Zhou K, Han J, Lund H, Boggavarapu N, Lauschke V, Goto S, et al. An overlooked subset of Cx3cr1wt/wt microglia in the Cx3cr1CreER-Eyfp/wt mouse has a repopulation advantage over Cx3cr1CreER-Eyfp/wt microglia following microglial depletion. J Neuroinflammation. 2022;19:20 pubmed publisher
Park E, Myint P, Appiah M, Worawattananutai P, Inprasit J, Prajuabjinda O, et al. Ligand-competent fractalkine receptor is expressed on exosomes. Biochem Biophys Rep. 2021;26:100932 pubmed publisher
Otobe S, Hisamoto T, Miyagaki T, Morimura S, Suga H, Sugaya M, et al. CX3CR1 Deficiency Attenuates DNFB-Induced Contact Hypersensitivity Through Skewed Polarization Towards M2 Phenotype in Macrophages. Int J Mol Sci. 2020;21: pubmed publisher
Mizoguchi M, Ishida Y, Nosaka M, Kimura A, Kuninaka Y, Yahata T, et al. Prevention of lipopolysaccharide-induced preterm labor by the lack of CX3CL1-CX3CR1 interaction in mice. PLoS ONE. 2018;13:e0207085 pubmed publisher
Zhang Y, Zhao L, Wang X, Ma W, Lazere A, Qian H, et al. Repopulating retinal microglia restore endogenous organization and function under CX3CL1-CX3CR1 regulation. Sci Adv. 2018;4:eaap8492 pubmed publisher
Kemmerling N, Wunderlich P, Theil S, Linnartz Gerlach B, Hersch N, Hoffmann B, et al. Intramembranous processing by ?-secretase regulates reverse signaling of ephrin-B2 in migration of microglia. Glia. 2017;65:1103-1118 pubmed publisher
Roche S, Wyse Jackson A, Gomez Vicente V, Lax P, Ruiz Lopez A, Byrne A, et al. Progesterone Attenuates Microglial-Driven Retinal Degeneration and Stimulates Protective Fractalkine-CX3CR1 Signaling. PLoS ONE. 2016;11:e0165197 pubmed publisher
McComb J, Ranganathan M, Liu X, Pilewski J, Ray P, Watkins S, et al. CX3CL1 up-regulation is associated with recruitment of CX3CR1+ mononuclear phagocytes and T lymphocytes in the lungs during cigarette smoke-induced emphysema. Am J Pathol. 2008;173:949-61 pubmed publisher
Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, et al. Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis. J Immunol. 2008;181:4208-18 pubmed
Cardona A, Sasse M, Liu L, Cardona S, Mizutani M, Savarin C, et al. Scavenging roles of chemokine receptors: chemokine receptor deficiency is associated with increased levels of ligand in circulation and tissues. Blood. 2008;112:256-63 pubmed publisher
Lee F, Haskell C, Charo I, Boettiger D. Receptor-ligand binding in the cell-substrate contact zone: a quantitative analysis using CX3CR1 and CXCR1 chemokine receptors. Biochemistry. 2004;43:7179-86 pubmed

product information

master code :

472-FF

SKU :

472-FF-025

product name :

Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein

unit size :

25 ug

description :

The Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein from R&D Systems is derived from Sf 21 (baculovirus). The Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein has been validated for the following applications: Bioactivity.

target :

CX3CL1/Fractalkine

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Mouse

observed molecular weight :

90 kDa, reducing conditions

theoretical molecular weight :

34 kDa

gene symbol :

Cx3cl1

details of functionality :

Measured by its ability to chemoattract freshly isolated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.2-0.6 ╡g/mL. Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED50 for this effect is 0.03-0.12 ╡g/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

O35188

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 21 (baculovirus)-derived mouse CX3CL1/Fractalkine protein Gln25-Arg337, with a C-terminal 6-His tag

source short :

Sf 21 (baculovirus)

USD :

432 USD

alt names :

ABCD-3, C3Xkine, chemokine (C-X3-C motif) ligand 1, CX3C membrane-anchored chemokine, CXC3, CXC3C, FKN, fractalkine, Neurotactin, neurotactin), NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, SCYD1C-X3-C motif chemokine 1, small inducible cytokine subfamily D (Cys-X3-Cys), member-1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage