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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human R-Spondin 1 Protein, CF
catalog :
4645-RS-025/CF
quantity :
25 ug
price :
377 USD
more info or order :
citations: 79
Reference
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Nanki Y, Chiyoda T, Hirasawa A, Ookubo A, Itoh M, Ueno M, et al. Patient-derived ovarian cancer organoids capture the genomic profiles of primary tumours applicable for drug sensitivity and resistance testing. Sci Rep. 2020;10:12581 pubmed publisher
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Zhang M, Haughey M, Wang N, Blease K, Kapoun A, Couto S, et al. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs. PLoS ONE. 2020;15:e0229445 pubmed publisher
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Levin G, Koga B, Belchior G, Carreira A, Sogayar M. Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells. BMC Biotechnol. 2020;20:5 pubmed publisher
Syed S, Kumar M, Ghosh A, Tomasetig F, Ali A, Whan R, et al. Endometrial Axin2+ Cells Drive Epithelial Homeostasis, Regeneration, and Cancer following Oncogenic Transformation. Cell Stem Cell. 2020;26:64-80.e13 pubmed publisher
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Tysoe O, Justin A, Brevini T, Chen S, Mahbubani K, Frank A, et al. Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue. Nat Protoc. 2019;: pubmed publisher
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Wong H, Qin H, Tsang S, Zuo X, Che S, Chow C, et al. Early life stress disrupts intestinal homeostasis via NGF-TrkA signaling. Nat Commun. 2019;10:1745 pubmed publisher
Konagaya S, Iwata H. Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells. Sci Rep. 2019;9:640 pubmed publisher
Zwarycz B, Gracz A, Rivera K, Williamson I, Samsa L, Starmer J, et al. IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model. Cell Mol Gastroenterol Hepatol. 2019;7:1-17 pubmed publisher
Scavuzzo M, Chmielowiec J, Yang D, Wamble K, Chaboub L, Duraine L, et al. Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA. Cell Rep. 2018;25:3811-3827.e7 pubmed publisher
Barros Silva J, Linn D, Steiner I, Guo G, Ali A, Pakula H, et al. Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer. Cell Rep. 2018;25:3504-3518.e6 pubmed publisher
Cruz Acuña R, Quirós M, Huang S, Siuda D, Spence J, Nusrat A, et al. PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery. Nat Protoc. 2018;13:2102-2119 pubmed publisher
Park S, Cui J, Yu W, Wu L, Carmon K, Liu Q. Differential activities and mechanisms of the four R-spondins in potentiating Wnt/β-catenin signaling. J Biol Chem. 2018;293:9759-9769 pubmed publisher
Chen Y, Tsai C, Wei Y, Wu Y, Hsu W, Lin H, et al. ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA. Cell Death Dis. 2018;9:437 pubmed publisher
Li J, Zhang S, Li R, Lin X, Mi Y, Zhang C. Culture and characterization of chicken small intestinal crypts. Poult Sci. 2018;97:1536-1543 pubmed publisher
Lebensohn A, Rohatgi R. R-spondins can potentiate WNT signaling without LGRs. elife. 2018;7: pubmed publisher
Lee N, Zhang Y, Su Y, Bidlingmaier S, Sherbenou D, Ha K, et al. Cell-type specific potent Wnt signaling blockade by bispecific antibody. Sci Rep. 2018;8:766 pubmed publisher
Fumagalli A, Suijkerbuijk S, Begthel H, Beerling E, Oost K, Snippert H, et al. A surgical orthotopic organoid transplantation approach in mice to visualize and study colorectal cancer progression. Nat Protoc. 2018;13:235-247 pubmed publisher
Taguchi A, Nishinakamura R. Higher-Order Kidney Organogenesis from Pluripotent Stem Cells. Cell Stem Cell. 2017;21:730-746.e6 pubmed publisher
Zhou X, Geng L, Wang D, Yi H, Talmon G, Wang J. R-Spondin1/LGR5 Activates TGFβ Signaling and Suppresses Colon Cancer Metastasis. Cancer Res. 2017;77:6589-6602 pubmed publisher
Isshiki H, Arimura Y, Nagaishi K, Kawakami K, Onodera K, Yamashita K, et al. Establishment of a refined culture method for rat colon organoids. Biochem Biophys Res Commun. 2017;489:305-311 pubmed publisher
Gao Y, Cai A, Xi H, Li J, Xu W, Zhang Y, et al. Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway. Stem Cell Res Ther. 2017;8:98 pubmed publisher
Han S, Shim S, Kim M, Shin H, Jang W, Lee S, et al. Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor. World J Gastroenterol. 2017;23:964-975 pubmed publisher
Chen W, Ju S, Lu T, Xu Y, Zheng X, Wang H, et al. Directional delivery of RSPO1 by mesenchymal stem cells ameliorates radiation-induced intestinal injury. Cytokine. 2017;95:27-34 pubmed publisher
Nigmatullina L, Norkin M, Dzama M, Messner B, Sayols S, Soshnikova N. Id2 controls specification of Lgr5+ intestinal stem cell progenitors during gut development. EMBO J. 2017;36:869-885 pubmed publisher
Lebensohn A, Dubey R, Neitzel L, Tacchelly Benites O, Yang E, Marceau C, et al. Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling. elife. 2016;5: pubmed publisher
Fujii S, Suzuki K, Kawamoto A, Ishibashi F, Nakata T, Murano T, et al. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells. Sci Rep. 2016;6:36795 pubmed publisher
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Jackson H, Granger D, Jones G, Anderson L, Friel S, Rycroft D, et al. Novel Bispecific Domain Antibody to LRP6 Inhibits Wnt and R-spondin Ligand-Induced Wnt Signaling and Tumor Growth. Mol Cancer Res. 2016;14:859-68 pubmed publisher
Rouch J, Scott A, Lei N, Solorzano Vargas R, Wang J, Hanson E, et al. Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids. PLoS ONE. 2016;11:e0148216 pubmed publisher
Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee Hoskins C, et al. Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates β-Catenin Signaling and Tumorigenesis in Multiple Cancer Types. Cancer Res. 2016;76:713-23 pubmed publisher
James M, Howells L, Karmokar A, Higgins J, Greaves P, Cai H, et al. Characterization and propagation of tumor initiating cells derived from colorectal liver metastases: trials, tribulations and a cautionary note. PLoS ONE. 2015;10:e0117776 pubmed publisher
Jabaji Z, Brinkley G, Khalil H, Sears C, Lei N, Lewis M, et al. Type I collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium. PLoS ONE. 2014;9:e107814 pubmed publisher
Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, et al. Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium. J Immunol. 2014;193:439-51 pubmed publisher
Yimlamai D, Christodoulou C, Galli G, Yanger K, Pepe Mooney B, Gurung B, et al. Hippo pathway activity influences liver cell fate. Cell. 2014;157:1324-38 pubmed publisher
Carmon K, Gong X, Yi J, Thomas A, Liu Q. RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. Proc Natl Acad Sci U S A. 2014;111:E1221-9 pubmed publisher
Guezguez A, Paré F, Benoit Y, Basora N, Beaulieu J. Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Exp Cell Res. 2014;322:355-64 pubmed publisher
Ronaghi M, Nasr M, Ealy M, Durruthy Durruthy R, Waldhaus J, Diaz G, et al. Inner ear hair cell-like cells from human embryonic stem cells. Stem Cells Dev. 2014;23:1275-84 pubmed publisher
Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, et al. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun. 2014;5:3149 pubmed publisher
Lei N, Jabaji Z, Wang J, Joshi V, Brinkley G, Khalil H, et al. Intestinal subepithelial myofibroblasts support the growth of intestinal epithelial stem cells. PLoS ONE. 2014;9:e84651 pubmed publisher
Lee J, Sugiyama T, Liu Y, Wang J, Gu X, Lei J, et al. Expansion and conversion of human pancreatic ductal cells into insulin-secreting endocrine cells. elife. 2013;2:e00940 pubmed publisher
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Hao H, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, et al. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature. 2012;485:195-200 pubmed publisher
Demidov O, Zhu Y, Kek C, Goloudina A, Motoyama N, Bulavin D. Role of Gadd45a in Wip1-dependent regulation of intestinal tumorigenesis. Cell Death Differ. 2012;19:1761-8 pubmed publisher
Spence J, Mayhew C, Rankin S, Kuhar M, Vallance J, Tolle K, et al. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature. 2011;470:105-9 pubmed publisher
image
image 1 :
R&D Systems 4645-RS-025/CF image 1
Recombinant Human R-Spondin 1 Protein Bioactivity
product information
brand :
R&D Systems
master code :
4645-RS/CF
SKU :
4645-RS-025/CF
product name :
Recombinant Human R-Spondin 1 Protein, CF
unit size :
25 ug
description :
P-gp and BCRP inhibitor
target :
R-Spondin 1
category :
Proteins and Enzymes
alt names :
Cristin 3, CRISTIN3, FLJ40906Roof plate-specific spondin-1, hRspo1, roof plate-specific spondin, RSPO, RSPO1, RSPONDIN, RSpondin 1, R-spondin homolog (Xenopus laevis), R-spondin1, R-spondin-1
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS.
conjugate :
Unconjugated
all available conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
observed molecular weight :
39 kDa, reducing conditions
theoretical molecular weight :
25.6 kDa
gene symbol :
RSPO1
details of functionality :
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1-10 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
Q2MKA7
applications :
Bioactivity
source :
CHO-derived Recombinant Human R-Spondin 1 Protein, CF
extended description :
Earn double rewards for reviews
USD :
349
Jul-22 :
377 USD
product details :
Earn double rewards for reviews
sizes available :
25 ug (also 100 ug, 1 mg)
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.