product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF
catalog :
464-SH-025/CF
quantity :
25 ug (also 200 ug)
price :
390 USD
more info or order :
citations: 60
Reference
Fan Y, Hackland J, Baggiolini A, Hung L, Zhao H, Zumbo P, et al. hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease. Cell Stem Cell. 2023;30:264-282.e9 pubmed publisher
Corenblum M, McRobbie Johnson A, Carruth E, Bernard K, Luo M, Mandarino L, et al. Parallel Neurodegenerative Phenotypes in Sporadic Parkinson's Disease Fibroblasts and Midbrain Dopamine Neurons. bioRxiv. 2023;: pubmed publisher
Wu P, Chiang S, Midence R, Kao W, Lai C, Cheng I, et al. Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation. Cell Death Dis. 2023;14:52 pubmed publisher
Wu S, Hernandez Villegas N, Sirkis D, Thomas Wright I, Wade Martins R, Schekman R. Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers. elife. 2023;12: pubmed publisher
Iannielli A, Luoni M, Giannelli S, Ferese R, Ordazzo G, Fossati M, et al. Modeling native and seeded Synuclein aggregation and related cellular dysfunctions in dopaminergic neurons derived by a new set of isogenic iPSC lines with SNCA multiplications. Cell Death Dis. 2022;13:881 pubmed publisher
Moriarty N, Gantner C, Hunt C, Ermine C, Frausin S, Viventi S, et al. A combined cell and gene therapy approach for homotopic reconstruction of midbrain dopamine pathways using human pluripotent stem cells. Cell Stem Cell. 2022;29:434-448.e5 pubmed publisher
Tang C, Wu X, Ren Q, Yao M, Xu S, Yan Z. Hedgehog signaling is controlled by Rac1 activity. Theranostics. 2022;12:1303-1320 pubmed publisher
Earley A, Burbulla L, Krainc D, Awatramani R. Identification of ASCL1 as a determinant for human iPSC-derived dopaminergic neurons. Sci Rep. 2021;11:22257 pubmed publisher
de Rus Jacquet A, Tancredi J, Lemire A, DeSantis M, Li W, O Shea E. The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson's disease. elife. 2021;10: pubmed publisher
De Luzy I, Law K, Moriarty N, Hunt C, Durnall J, Thompson L, et al. Human stem cells harboring a suicide gene improve the safety and standardisation of neural transplants in Parkinsonian rats. Nat Commun. 2021;12:3275 pubmed publisher
Padmanabhan Nair V, Liu H, Ciceri G, Jungverdorben J, Frishman G, Tchieu J, et al. Activation of HERV-K(HML-2) disrupts cortical patterning and neuronal differentiation by increasing NTRK3. Cell Stem Cell. 2021;28:1566-1581.e8 pubmed publisher
Tao Y, Vermilyea S, Zammit M, Lu J, Olsen M, Metzger J, et al. Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys. Nat Med. 2021;27:632-639 pubmed publisher
Kim T, Piao J, Koo S, Kriks S, Chung S, Betel D, et al. Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use. Cell Stem Cell. 2021;28:343-355.e5 pubmed publisher
Piao J, Zabierowski S, Dubose B, Hill E, Navare M, Claros N, et al. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01. Cell Stem Cell. 2021;28:217-229.e7 pubmed publisher
Kim J, Yin X, Jhaldiyal A, Khan M, Martin I, Xie Z, et al. Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis. Cell Stem Cell. 2020;27:633-645.e7 pubmed publisher
Yang L, Han Y, Nilsson Payant B, Gupta V, Wang P, Duan X, et al. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids. Cell Stem Cell. 2020;27:125-136.e7 pubmed publisher
Chen K, Cheng C, Cheng C, Jhan K, Chen Y, Wang L. The excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis induces autophagy via the Sonic hedgehog pathway in mouse brain astrocytes. PLoS Negl Trop Dis. 2020;14:e0008290 pubmed publisher
Aldaregia J, Errarte P, Olazagoitia Garmendia A, Gimeno M, Uriz J, Gershon T, et al. Erbb4 Is Required for Cerebellar Developmentand Malignant Phenotype of Medulloblastoma. Cancers (Basel). 2020;12: pubmed publisher
Kasiri S, Chen B, Wilson A, Reczek A, Mazambani S, Gadhvi J, et al. Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species. Oncogene. 2020;39:3258-3275 pubmed publisher
Vermilyea S, Babinski A, Tran N, To S, Guthrie S, Kluss J, et al. In Vitro CRISPR/Cas9-Directed Gene Editing to Model LRRK2 G2019S Parkinson's Disease in Common Marmosets. Sci Rep. 2020;10:3447 pubmed publisher
Wada Y, Kikuchi A, Kaga A, Shimizu N, Ito J, Onuma R, et al. Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice. PLoS Genet. 2020;16:e1008628 pubmed publisher
Gantner C, De Luzy I, Kauhausen J, Moriarty N, Niclis J, Bye C, et al. Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease. Cell Stem Cell. 2020;26:511-526.e5 pubmed publisher
Mahajani S, Raina A, Fokken C, Kügler S, Bahr M. Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors. Cell Death Dis. 2019;10:898 pubmed publisher
de Rus Jacquet A. Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes. Curr Protoc Cell Biol. 2019;85:e98 pubmed publisher
Leitner D, Ramamoorthy M, Dejosez M, Zwaka T. Immature mDA neurons ameliorate motor deficits in a 6-OHDA Parkinson's disease mouse model and are functional after cryopreservation. Stem Cell Res. 2019;41:101617 pubmed publisher
De Luzy I, Niclis J, Gantner C, Kauhausen J, Hunt C, Ermine C, et al. Isolation of LMX1a Ventral Midbrain Progenitors Improves the Safety and Predictability of Human Pluripotent Stem Cell-Derived Neural Transplants in Parkinsonian Disease. J Neurosci. 2019;39:9521-9531 pubmed publisher
Riessland M, Kolisnyk B, Kim T, Cheng J, Ni J, Pearson J, et al. Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons. Cell Stem Cell. 2019;25:514-530.e8 pubmed publisher
Penas C, Maloof M, Stathias V, Long J, Tan S, Mier J, et al. Time series modeling of cell cycle exit identifies Brd4 dependent regulation of cerebellar neurogenesis. Nat Commun. 2019;10:3028 pubmed publisher
Xiang Y, Tanaka Y, Cakir B, Patterson B, Kim K, Sun P, et al. hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids. Cell Stem Cell. 2019;: pubmed publisher
Grabow S, Kueh A, Ke F, Vanyai H, Sheikh B, Dengler M, et al. Subtle Changes in the Levels of BCL-2 Proteins Cause Severe Craniofacial Abnormalities. Cell Rep. 2018;24:3285-3295.e4 pubmed publisher
Tasnim N, Thakur V, Chattopadhyay M, Joddar B. The Efficacy of Graphene Foams for Culturing Mesenchymal Stem Cells and Their Differentiation into Dopaminergic Neurons. Stem Cells Int. 2018;2018:3410168 pubmed publisher
Everson J, Fink D, Chung H, Sun M, Lipinski R. Identification of sonic hedgehog-regulated genes and biological processes in the cranial neural crest mesenchyme by comparative transcriptomics. BMC Genomics. 2018;19:497 pubmed publisher
Wang B, Joo J, Mount R, Teubner B, Krenzer A, Ward A, et al. The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest. 2018;128:3319-3332 pubmed publisher
Collo G, Cavalleri L, Zoli M, Maskos U, Ratti E, Merlo Pich E. Alpha6-Containing Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Structural Plasticity in Mouse and Human iPSC-Derived Dopaminergic Neurons. Front Pharmacol. 2018;9:572 pubmed publisher
Wang Y, Zhu W, Wu M, Wu Y, Liu Z, Liang L, et al. Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons Recover Locomotive Defects of Nonhuman Primate Models of Parkinson's Disease. Stem Cell Reports. 2018;11:171-182 pubmed publisher
Collo G, Cavalleri L, Bono F, Mora C, Fedele S, Invernizzi R, et al. Ropinirole and Pramipexole Promote Structural Plasticity in Human iPSC-Derived Dopaminergic Neurons via BDNF and mTOR Signaling. Neural Plast. 2018;2018:4196961 pubmed publisher
Adil M, Schaffer D. hPSC-derived Midbrain Dopaminergic Neurons Generated in a Scalable 3-D Biomaterial. Curr Protoc Stem Cell Biol. 2018;44:2D.21.1-2D.21.17 pubmed publisher
Niclis J, Gantner C, Hunt C, Kauhausen J, Durnall J, Haynes J, et al. A PITX3-EGFP Reporter Line Reveals Connectivity of Dopamine and Non-dopamine Neuronal Subtypes in Grafts Generated from Human Embryonic Stem Cells. Stem Cell Reports. 2017;9:868-882 pubmed publisher
Han Y, Xiong Y, Shi X, Wu J, Zhao Y, Jiang J. Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system. PLoS Biol. 2017;15:e2002063 pubmed publisher
Xiang Y, Tanaka Y, Patterson B, Kang Y, Govindaiah G, Roselaar N, et al. Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration. Cell Stem Cell. 2017;21:383-398.e7 pubmed publisher
Baronchelli S, La Spada A, Ntai A, Barbieri A, Conforti P, Jotti G, et al. Epigenetic and transcriptional modulation of WDR5, a chromatin remodeling protein, in Huntington's disease human induced pluripotent stem cell (hiPSC) model. Mol Cell Neurosci. 2017;82:46-57 pubmed publisher
Niclis J, Gantner C, Alsanie W, McDougall S, Bye C, Elefanty A, et al. Efficiently Specified Ventral Midbrain Dopamine Neurons from Human Pluripotent Stem Cells Under Xeno-Free Conditions Restore Motor Deficits in Parkinsonian Rodents. Stem Cells Transl Med. 2017;6:937-948 pubmed publisher
Liddelow S, Guttenplan K, Clarke L, Bennett F, Bohlen C, Schirmer L, et al. Neurotoxic reactive astrocytes are induced by activated microglia. Nature. 2017;541:481-487 pubmed publisher
Heyne G, Everson J, Ansen Wilson L, Melberg C, Fink D, Parins K, et al. Gli2 gene-environment interactions contribute to the etiological complexity of holoprosencephaly: evidence from a mouse model. Dis Model Mech. 2016;9:1307-1315 pubmed
Shackleford G, Shi X, Swanson K, Mahdi M, Gonzalez Gomez I, Asgharzadeh S, et al. BarTeL, a Genetically Versatile, Bioluminescent and Granule Neuron Precursor-Targeted Mouse Model for Medulloblastoma. PLoS ONE. 2016;11:e0156907 pubmed publisher
Cao J, Poss K. Explant culture of adult zebrafish hearts for epicardial regeneration studies. Nat Protoc. 2016;11:872-81 pubmed publisher
Wang J, Cao J, Dickson A, Poss K. Epicardial regeneration is guided by cardiac outflow tract and Hedgehog signalling. Nature. 2015;522:226-230 pubmed publisher
Shi Y, Chen J, Karner C, Long F. Hedgehog signaling activates a positive feedback mechanism involving insulin-like growth factors to induce osteoblast differentiation. Proc Natl Acad Sci U S A. 2015;112:4678-83 pubmed publisher
Edri R, Yaffe Y, Ziller M, Mutukula N, Volkman R, David E, et al. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun. 2015;6:6500 pubmed publisher
Aguila J, Blak A, van Arensbergen J, Sousa A, Vazquez N, Aduriz A, et al. Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for Dopamine Neurons From Human Pluripotent Stem Cells. Stem Cells Transl Med. 2014;3:1032-42 pubmed publisher
Peluso M, Campbell V, Harari J, Tibbitts T, Proctor J, Whitebread N, et al. Impact of the Smoothened inhibitor, IPI-926, on smoothened ciliary localization and Hedgehog pathway activity. PLoS ONE. 2014;9:e90534 pubmed publisher
Luo W, Rodriguez M, Valdez J, Zhu X, Tan K, Li D, et al. Lgr4 is a key regulator of prostate development and prostate stem cell differentiation. Stem Cells. 2013;31:2492-505 pubmed publisher
Maroof A, Keros S, Tyson J, Ying S, Ganat Y, Merkle F, et al. Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells. Cell Stem Cell. 2013;12:559-72 pubmed publisher
Najm F, Lager A, Zaremba A, Wyatt K, Caprariello A, Factor D, et al. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells. Nat Biotechnol. 2013;31:426-33 pubmed publisher
Ho L, Ali S, Al Jazrawe M, Kandel R, Wunder J, Alman B. Primary cilia attenuate hedgehog signalling in neoplastic chondrocytes. Oncogene. 2013;32:5388-96 pubmed publisher
Garcia I, Crowther A, Gama V, Miller C, Miller C, Deshmukh M, et al. Bax deficiency prolongs cerebellar neurogenesis, accelerates medulloblastoma formation and paradoxically increases both malignancy and differentiation. Oncogene. 2013;32:2304-14 pubmed publisher
Krencik R, Weick J, Liu Y, Zhang Z, Zhang S. Specification of transplantable astroglial subtypes from human pluripotent stem cells. Nat Biotechnol. 2011;29:528-34 pubmed publisher
Zeng H, Guo M, Martins Taylor K, Wang X, Zhang Z, Park J, et al. Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells. PLoS ONE. 2010;5:e11853 pubmed publisher
Nolan Stevaux O, Lau J, Truitt M, Chu G, Hebrok M, Fernandez Zapico M, et al. GLI1 is regulated through Smoothened-independent mechanisms in neoplastic pancreatic ducts and mediates PDAC cell survival and transformation. Genes Dev. 2009;23:24-36 pubmed publisher
Bastida M, Delgado M, Wang B, Fallon J, Fernandez Teran M, Ros M. Levels of Gli3 repressor correlate with Bmp4 expression and apoptosis during limb development. Dev Dyn. 2004;231:148-60 pubmed
image
image 1 :
R&D Systems 464-SH-025/CF image 1
Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus Bioactivity
product information
brand :
R&D Systems, a Bio-Techne brand
catalog number base :
464-SH/CF
SKU :
464-SH-025/CF
product name :
Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF
description :
The Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF from R&D Systems is derived from E. coli. The Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF has been validated for the following applications: Bioactivity.
target :
Sonic Hedgehog/Shh
unit size :
25 ug (also 200 ug)
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 µm filtered solution in NaH2PO4, NaCl and DTT.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Mouse
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
theoretical molecular weight :
19.8 kDa
gene symbol :
Shh
details of functionality :
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al . (1997) Biochem. Biophys. Res. Commun. 237 :465. The ED50 for this effect is 0.05-0.25 µg/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
Q62226
applications :
Bioactivity
product keywords :
464-SH, rmShh-N C25II Substitute, mShh-N (C25II Substitute), carrier-free, carrier free, 464SH/CF, cytokinegrowthfact, Sonic Hedgehog Signaling Molecule, Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains, ShhNC, Sonic Hedgehog Homolog, MCOPCB5, SMMCI, TPTPS, HHG-1, HHG1, HLP3, HPE3, TPT
source :
E. coli-derived Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus, CF
USD :
386
USD 2025 :
390 USD
alt names :
HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.