Recombinant Human R-Spondin 4 Protein | R&D Systems 4575-RS-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human R-Spondin 4 Protein

catalog :

4575-RS-025

quantity :

25 ug

price :

499 USD

more info or order :

R&D Systems product webpage

citations: 8

Reference
Nualart F, Cifuentes M, Ram xed rez E, Mart xed nez F, Barahona M, Ferrada L, et al. Hyperglycemia increases SCO-spondin and Wnt5a secretion into the cerebrospinal fluid to regulate ependymal cell beating and glucose sensing. PLoS Biol. 2023;21:e3002308 pubmed publisher
Lee H, Seidl C, Sun R, Glinka A, Niehrs C. R-spondins are BMP receptor antagonists in Xenopus early embryonic development. Nat Commun. 2020;11:5570 pubmed publisher
Park S, Cui J, Yu W, Wu L, Carmon K, Liu Q. Differential activities and mechanisms of the four R-spondins in potentiating Wnt/β-catenin signaling. J Biol Chem. 2018;293:9759-9769 pubmed publisher
Lebensohn A, Rohatgi R. R-spondins can potentiate WNT signaling without LGRs. elife. 2018;7: pubmed publisher
Carmon K, Gong X, Yi J, Thomas A, Liu Q. RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. Proc Natl Acad Sci U S A. 2014;111:E1221-9 pubmed publisher
Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, et al. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun. 2014;5:3149 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Hao H, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, et al. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature. 2012;485:195-200 pubmed publisher

product information

brand :

R&D Systems, a Bio-Techne Brand

catalog number base :

4575-RS

SKU :

4575-RS-025

product name :

Recombinant Human R-Spondin 4 Protein

Description :

The Recombinant Human R-Spondin 4 Protein from R&D Systems is derived from CHO. The Recombinant Human R-Spondin 4 Protein has been validated for the following applications: Bioactivity.

target :

R-Spondin 4

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

30-36 kDa, reducing conditions

theoretical molecular weight :

24.1 kDa (monomer)

gene symbol :

RSPO4

details of functionality :

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 10-60 ng/mL in the presence of 10 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

Q2I0M5

applications :

Bioactivity

source :

CHO-derived Recombinant Human R-Spondin 4 Protein

USD :

499 USD

alt names :

C20orf182, chromosome 20 open reading frame 182, Cristin 4, CRISTIN4, dJ824F16.3, FLJ16018, hRspo4, Roof plate-specific spondin-4, RSPO4, RSpondin 4, R-spondin family, member 4, R-spondin-4

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage