product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse CCL21/6Ckine Protein
catalog :
457-6C-025
quantity :
25 ug
price :
359 USD
more info or order :
R&D Systems product webpage
citations: 42
Reference
Nakatani T, Tsujimoto K, Park J, Jo T, Kimura T, Hayama Y, et al. The lysosomal Ragulator complex plays an essential role in leukocyte trafficking by activating myosin II. Nat Commun. 2021;12:3333 pubmed publisher
Mao F, Lv Y, Hao C, Teng Y, Liu Y, Cheng P, et al. Helicobacter pylori-Induced Rev-erbα Fosters Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense. Cell Mol Gastroenterol Hepatol. 2021;12:395-425 pubmed publisher
Trempolec N, Doix B, Degavre C, Brusa D, Bouzin C, Riant O, et al. Photodynamic Therapy-Based Dendritic Cell Vaccination Suited to Treat Peritoneal Mesothelioma. Cancers (Basel). 2020;12: pubmed publisher
Li L, Shirkey M, Zhang T, Xiong Y, Piao W, Saxena V, et al. The lymph node stromal laminin α5 shapes alloimmunity. J Clin Invest. 2020;130:2602-2619 pubmed publisher
Sharif S, Ueda N, Nakatani Y, Wise L, Clifton S, Lateef Z, et al. Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity. Front Microbiol. 2019;10:1421 pubmed publisher
Capitano M, Mor Vaknin N, Saha A, Cooper S, Legendre M, Guo H, et al. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling. J Clin Invest. 2019;129:2555-2570 pubmed publisher
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
Moretti F, Klapproth S, Ruppert R, Margraf A, Weber J, Pick R, et al. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus. elife. 2018;7: pubmed publisher
Piao W, Xiong Y, Famulski K, Brinkman C, Li L, Toney N, et al. Regulation of T cell afferent lymphatic migration by targeting LTβR-mediated non-classical NFκB signaling. Nat Commun. 2018;9:3020 pubmed publisher
Mashud R, Nomachi A, Hayakawa A, Kubouchi K, Danno S, Hirata T, et al. Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1. Sci Rep. 2017;7:7663 pubmed publisher
Burbage M, Keppler S, Montaner B, Mattila P, Batista F. The Small Rho GTPase TC10 Modulates B Cell Immune Responses. J Immunol. 2017;199:1682-1695 pubmed publisher
Saito T, Yano M, Ohki Y, Tomura M, Nakano N. Occludin Expression in Epidermal ?? T Cells in Response to Epidermal Stress Causes Them To Migrate into Draining Lymph Nodes. J Immunol. 2017;199:62-71 pubmed publisher
Shiraishi A, Uruno T, Sanematsu F, Ushijima M, Sakata D, Hara T, et al. DOCK8 Protein Regulates Macrophage Migration through Cdc42 Protein Activation and LRAP35a Protein Interaction. J Biol Chem. 2017;292:2191-2202 pubmed publisher
Takács E, Boto P, Simo E, Csuth T, Toth B, Raveh Amit H, et al. Immunogenic Dendritic Cell Generation from Pluripotent Stem Cells by Ectopic Expression of Runx3. J Immunol. 2017;198:239-248 pubmed
Biajoux V, Natt J, Freitas C, Alouche N, Sacquin A, Hemon P, et al. Efficient Plasma Cell Differentiation and Trafficking Require Cxcr4 Desensitization. Cell Rep. 2016;17:193-205 pubmed publisher
Roufaiel M, Gracey E, Siu A, Zhu S, Lau A, Ibrahim H, et al. CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima. Nat Immunol. 2016;17:1263-1272 pubmed publisher
Parzmair G, Gereke M, Haberkorn O, Annemann M, Podlasly L, Kliche S, et al. ADAP plays a pivotal role in CD4+ T cell activation but is only marginally involved in CD8+ T cell activation, differentiation, and immunity to pathogens. J Leukoc Biol. 2017;101:407-419 pubmed publisher
Köchl R, Thelen F, Vanes L, Brazão T, Fountain K, Xie J, et al. WNK1 kinase balances T cell adhesion versus migration in vivo. Nat Immunol. 2016;17:1075-83 pubmed publisher
Lo T, Silveira P, Fromm P, Verma N, Vu P, Kupresanin F, et al. Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration. J Immunol. 2016;197:885-98 pubmed publisher
Gómez D, Diehl M, Crosby E, Weinkopff T, Debes G. Effector T Cell Egress via Afferent Lymph Modulates Local Tissue Inflammation. J Immunol. 2015;195:3531-6 pubmed publisher
Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, et al. TGF-β1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis. Oncogene. 2016;35:748-60 pubmed publisher
Badr G, Sayed A, Abdel Maksoud M, Mohamed A, El Amir A, Abdel Ghaffar F, et al. Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFκB and ERK. PLoS ONE. 2015;10:e0125340 pubmed publisher
Ballesteros A, Mentink Kane M, Warren J, Kaplan G, Dveksler G. Induction and activation of latent transforming growth factor-β1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). J Biol Chem. 2015;290:4422-31 pubmed publisher
Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K. Control of lymphocyte egress from lymph nodes through β2-adrenergic receptors. J Exp Med. 2014;211:2583-98 pubmed publisher
Knowlden S, Capece T, Popovic M, Chapman T, Rezaee F, Kim M, et al. Regulation of T cell motility in vitro and in vivo by LPA and LPA2. PLoS ONE. 2014;9:e101655 pubmed publisher
Katakai T, Kondo N, Ueda Y, Kinashi T. Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex. J Immunol. 2014;193:617-26 pubmed publisher
Geherin S, Wilson R, Jennrich S, Debes G. CXCR4 is dispensable for T cell egress from chronically inflamed skin via the afferent lymph. PLoS ONE. 2014;9:e95626 pubmed publisher
Xu Y, Pektor S, Balkow S, Hemkemeyer S, Liu Z, Grobe K, et al. Dendritic cell motility and T cell activation requires regulation of Rho-cofilin signaling by the Rho-GTPase activating protein myosin IXb. J Immunol. 2014;192:3559-68 pubmed publisher
Willinger T, Ferguson S, Pereira J, De Camilli P, Flavell R. Dynamin 2-dependent endocytosis is required for sustained S1PR1 signaling. J Exp Med. 2014;211:685-700 pubmed publisher
Nomachi A, Yoshinaga M, Liu J, Kanchanawong P, Tohyama K, Thumkeo D, et al. Moesin controls clathrin-mediated S1PR1 internalization in T cells. PLoS ONE. 2013;8:e82590 pubmed publisher
Luckashenak N, Wähe A, Breit K, Brakebusch C, Brocker T. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol. 2013;190:27-35 pubmed publisher
Hyun Y, Sumagin R, Sarangi P, Lomakina E, Overstreet M, Baker C, et al. Uropod elongation is a common final step in leukocyte extravasation through inflamed vessels. J Exp Med. 2012;209:1349-62 pubmed publisher
Ippagunta S, Malireddi R, Shaw P, Neale G, Vande Walle L, Green D, et al. The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization. Nat Immunol. 2011;12:1010-6 pubmed publisher
Rehm A, Mensen A, Schradi K, Gerlach K, Wittstock S, Winter S, et al. Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs. Blood. 2011;118:1020-33 pubmed publisher
Biber K, Tsuda M, Tozaki Saitoh H, Tsukamoto K, Toyomitsu E, Masuda T, et al. Neuronal CCL21 up-regulates microglia P2X4 expression and initiates neuropathic pain development. EMBO J. 2011;30:1864-73 pubmed publisher
Marttila Ichihara F, Turja R, Miiluniemi M, Karikoski M, Maksimow M, Niemela J, et al. Macrophage mannose receptor on lymphatics controls cell trafficking. Blood. 2008;112:64-72 pubmed publisher
Pham T, Okada T, Matloubian M, Lo C, Cyster J. S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress. Immunity. 2008;28:122-33 pubmed publisher
Kabashima K, Shiraishi N, Sugita K, Mori T, Onoue A, Kobayashi M, et al. CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells. Am J Pathol. 2007;171:1249-57 pubmed
Fujita H, Asahina A, Komine M, Tamaki K. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Cell Immunol. 2007;245:70-9 pubmed
Liu C, Ueno T, Kuse S, Saito F, Nitta T, Piali L, et al. The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood. 2005;105:31-9 pubmed
Flanagan K, Glover R, Horig H, Yang W, Kaufman H. Local delivery of recombinant vaccinia virus expressing secondary lymphoid chemokine (SLC) results in a CD4 T-cell dependent antitumor response. Vaccine. 2004;22:2894-903 pubmed
Tolba K, Bowers W, Muller J, Housekneckt V, Giuliano R, Federoff H, et al. Herpes simplex virus (HSV) amplicon-mediated codelivery of secondary lymphoid tissue chemokine and CD40L results in augmented antitumor activity. Cancer Res. 2002;62:6545-51 pubmed
product information
brand :
R&D Systems
master code :
457-6C
SKU :
457-6C-025
product name :
Recombinant Mouse CCL21/6Ckine Protein
description :
The CCL21/6Ckine Protein from 457-6C-025 is derived from E. coli-derived Recombinant Mouse CCL21/6Ckine Protein. The CCL21/6Ckine Protein has been validated for the following applications: P84444.1.
target :
CCL21/6Ckine
category :
Proteins and Enzymes
unit sizes :
25 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Mouse
observed molecular weight :
16-17 kDa, reducing conditions
theoretical molecular weight :
12 kDa
gene symbol :
Ccl21a
details of functionality :
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 50-200 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7. The ED50 for this effect is 3-15 ng/mL.
endotoxin note :
<0.01 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P84444.1
applications :
Bioactivity
source :
E. coli-derived Recombinant Mouse CCL21/6Ckine Protein
USD :
359 USD
alt names :
6Ckine, 6CkineSmall-inducible cytokine A21, Beta-chemokine exodus-2, chemokine (C-C motif) ligand 21, CKb9, exodus-2, member 21, SCYA21, SCYA21MGC34555, secondary lymphoid tissue chemokine, SLC, SLCSecondary lymphoid-tissue chemokine, TCA-4
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.