Recombinant Human Active Raf-1 (Y340E Y341E, 306-end), CF | R&D Systems 4540-KS-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Active Raf-1 (Y340E Y341E, 306-end), CF

catalog :

4540-KS-010

quantity :

10 ug

price :

534 USD

more info or order :

R&D Systems product webpage

citations: 2

Reference
Takahashi Niki K, Kato Ose I, Murata H, Maita H, Iguchi Ariga S, Ariga H. Epidermal Growth Factor-dependent Activation of the Extracellular Signal-regulated Kinase Pathway by DJ-1 Protein through Its Direct Binding to c-Raf Protein. J Biol Chem. 2015;290:17838-47 pubmed publisher
Hutchison M. BDNF alters ERK/p38 MAPK activity ratios to promote differentiation in growth plate chondrocytes. Mol Endocrinol. 2012;26:1406-16 pubmed publisher

product information

master code :

4540-KS

SKU :

4540-KS-010

product name :

Recombinant Human Active Raf-1 (Y340E Y341E, 306-end), CF

unit size :

10 ug

description :

The Recombinant Human Active Raf-1 (Y340E Y341E, 306-end), CF from R&D Systems is derived from Sf 9 (baculovirus). The Recombinant Human Active Raf-1 (Y340E Y341E, 306-end), CF has been validated for the following applications: Bioactivity.

target :

Raf-1

category :

Proteins and Enzymes

buffer :

Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM Glutathione, 0.1 mM EDTA, 0.1 mM PMSF, and 25% Glycerol.

conjugate :

Unconjugated

purity :

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie« Blue stain at 5 ╡g per lane.

species :

Human

observed molecular weight :

63 kDa

gene symbol :

RAF1

details of functionality :

The specific activity of Raf-1 is typically 5100-6900 nmol/min/mg using a myelin basic protein (MBP) substrate (see Activity Assay Protocol).

top caption :

Recombinant Human Active Raf-1 (Y340E Y341E, 306-end) SDS-PAGE

accessionNumbers :

NM_002880

applications :

Bioactivity

source long :

Spodoptera frugiperda , Sf 9 (baculovirus)-derived human Raf-1 protein aa 306-648

source short :

Sf 9 (baculovirus)

USD :

534 USD

alt names :

cRaf, c-Raf, c-Raf-1, CRAFraf proto-oncogene serine/threonine protein kinase, EC 2.7.11, EC 2.7.11.1, NS5, Proto-oncogene c-RAF, RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf1, Raf-1Oncogene RAF1, v-Raf-1, v-raf-1 murine leukemia viral oncogene homolog 1

storage :

This product is stable at Avoid repeated freeze-thaw cycles.

more info or order :

R&D Systems product webpage