Recombinant Mouse Neutrophil Elastase/ELA2 Protein, CF | R&D Systems 4517-SE-010 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse Neutrophil Elastase/ELA2 Protein, CF

catalog :

4517-SE-010

quantity :

10 ug

price :

432 USD

citations: 5

Reference
Chen D, Mirski M, Caton E, Kiser K, Haddaway C, Cetta M, et al. A Breath-Based In Vitro Diagnostics for Lower Respiratory Tract Infection. medRxiv. 2023;: pubmed publisher
Zhou L, Gao R, Hong H, Li X, Yang J, Shen W, et al. Emodin inhibiting neutrophil elastase-induced epithelial-mesenchymal transition through Notch1 signalling in alveolar epithelial cells. J Cell Mol Med. 2020;24:11998-12007 pubmed publisher
Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G. Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux. PLoS ONE. 2015;10:e0125797 pubmed publisher
Vicuña L, Strochlic D, Latremoliere A, Bali K, Simonetti M, Husainie D, et al. The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase. Nat Med. 2015;21:518-23 pubmed publisher
Talukdar S, Oh D, Bandyopadhyay G, Li D, Xu J, McNelis J, et al. Neutrophils mediate insulin resistance in mice fed a high-fat diet through secreted elastase. Nat Med. 2012;18:1407-12 pubmed

product information

master code :

4517-SE

SKU :

4517-SE-010

product name :

Recombinant Mouse Neutrophil Elastase/ELA2 Protein, CF

unit size :

10 ug

description :

The Recombinant Mouse Neutrophil Elastase/ELA2 Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse Neutrophil Elastase/ELA2 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

Neutrophil Elastase/ELA2

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris and NaCl.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Mouse

observed molecular weight :

34 kDa, reducing conditions

theoretical molecular weight :

27 kDa

gene symbol :

Elane

details of functionality :

Measured by its ability to cleave the fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC). The specific activity is 500 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_056594

applications :

Enzyme Activity

source long :

Mouse myeloma cell line, NS0-derived mouse Neutrophil Elastase/ELA2 protein Ser27-Arg260, with a C-terminal 10-His tag

source short :

NS0

USD :

432 USD

alt names :

Bone marrow serine protease, EC 3.4.21, EC 3.4.21.37, ELA2, ELA2granulocyte-derived elastase, ELANE, elastase 2, neutrophil, elastase, neutrophil expressed, Elastase-2, GE, HLEelastase-2, HNE, Human leukocyte elastase, Leukocyte Elastase, medullasin, NE, neutrophil elastase, PMN elastase, PMN-E, polymorphonuclear elastase, SCN1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.