Recombinant TEV Protease Protein, CF | R&D Systems 4469-TP-200 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant TEV Protease Protein, CF

catalog :

4469-TP-200

quantity :

200 ug

price :

217 USD

more info or order :

R&D Systems product webpage

citations: 2

Reference
Lakshmanan A, Jin Z, Nety S, Sawyer D, Lee Gosselin A, Malounda D, et al. Acoustic biosensors for ultrasound imaging of enzyme activity. Nat Chem Biol. 2020;16:988-996 pubmed publisher
Petrie M, Esquibel J, Kabachinski G, Maciuba S, Takahashi H, Edwardson J, et al. The Vesicle Priming Factor CAPS Functions as a Homodimer via C2 Domain Interactions to Promote Regulated Vesicle Exocytosis. J Biol Chem. 2016;291:21257-21270 pubmed

product information

master code :

4469-TP

SKU :

4469-TP-200

product name :

Recombinant TEV Protease Protein, CF

unit size :

200 ug

description :

The Recombinant TEV Protease Protein, CF from R&D Systems is derived from E. coli. The Recombinant TEV Protease Protein, CF has been validated for the following applications: Enzyme Activity.

target :

TEV Protease

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in Tris, NaCl, EDTA, DTT and Glycerol.

conjugate :

Unconjugated

purity :

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Viral

observed molecular weight :

25-30 kDa, reducing conditions

theoretical molecular weight :

28.5 kDa

details of functionality :

Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln, with the cleavage point after Gln. TEV Protease cleaves =50% of the control substrate, as measured under the described conditions. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_062908

applications :

Enzyme Activity

source long :

E. coli -derived viral TEV Protease protein Ala TEV Protease Glu2039-Gln2279 (Ser2256Asn) Accession # NP_062908 Leu 6-His tag N-terminus C-terminus

source short :

E. coli

USD :

217 USD

alt names :

NIa, P1 Protease, Tobacco Etch Virus Protease

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage