Recombinant Mouse EphB4 Fc Chimera Protein, CF | R&D Systems 446-B4-200 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse EphB4 Fc Chimera Protein, CF

catalog :

446-B4-200

quantity :

200 ug

price :

348 USD

citations: 26

Reference
Stewen J, Kruse K, Godoi Filip A, Zenia -, Jeong H, Adams S, et al. Eph-ephrin signaling couples endothelial cell sorting and arterial specification. Nat Commun. 2024;15:2539 pubmed publisher
Kohara S, Ogawa K. Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells. Biomedicines. 2022;10: pubmed publisher
Bissen D, Kracht M, Foss F, Hofmann J, Acker Palmer A. EphrinB2 and GRIP1 stabilize mushroom spines during denervation-induced homeostatic plasticity. Cell Rep. 2021;34:108923 pubmed publisher
Ge Y, Feng K, Liu X, Chen H, Sun Z, Wang C, et al. The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo. Oxid Med Cell Longev. 2020;2020:1404915 pubmed publisher
Yoon Y, Voloudakis G, Doran N, Zhang E, Dimovasili C, Chen L, et al. PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival. Mol Psychiatry. 2021;26:1996-2012 pubmed publisher
Wang W, Yuan C, Geng T, Liu Y, Zhu S, Zhang C, et al. EphrinB2 overexpression enhances osteogenic differentiation of dental pulp stem cells partially through ephrinB2-mediated reverse signaling. Stem Cell Res Ther. 2020;11:40 pubmed publisher
Harde E, Nicholson L, Furones Cuadrado B, Bissen D, Wigge S, Urban S, et al. EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development. elife. 2019;8: pubmed publisher
Grandi A, Zini I, Palese S, Giorgio C, Tognolini M, Marchesani F, et al. Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD. Front Pharmacol. 2019;10:691 pubmed publisher
Herrero D, Cañon S, Albericio G, Carmona R, Aguilar S, Manes S, et al. Age-related oxidative stress confines damage-responsive Bmi1+ cells to perivascular regions in the murine adult heart. Redox Biol. 2019;22:101156 pubmed publisher
Yoon J, Hwang Y, Lee M, Sun J, Cho H, Knapik L, et al. TBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration. Nat Commun. 2018;9:3491 pubmed publisher
Baek J, Cheon Y, Kwak S, Jun H, Yoon K, Lee M, et al. Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling. Exp Mol Med. 2018;50:50 pubmed publisher
Warren N, Voloudakis G, Yoon Y, Robakis N, Georgakopoulos A. The product of the γ-secretase processing of ephrinB2 regulates VE-cadherin complexes and angiogenesis. Cell Mol Life Sci. 2018;75:2813-2826 pubmed publisher
Ghori A, Freimann F, Nieminen Kelhä M, Kremenetskaia I, Gertz K, Endres M, et al. EphrinB2 Activation Enhances Vascular Repair Mechanisms and Reduces Brain Swelling After Mild Cerebral Ischemia. Arterioscler Thromb Vasc Biol. 2017;37:867-878 pubmed publisher
Greenblatt M, Park K, Oh H, Kim J, Shin D, Lee J, et al. CHMP5 controls bone turnover rates by dampening NF-ÎºB activity in osteoclasts. J Exp Med. 2015;212:1283-301 pubmed publisher
Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, et al. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling. Nat Commun. 2015;6:6576 pubmed publisher
White M, Rufaihah A, Liu L, Ghebremariam Y, Ivey K, Cooke J, et al. Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells. Stem Cells. 2013;31:92-103 pubmed publisher
Sawamiphak S, Seidel S, Essmann C, Wilkinson G, Pitulescu M, Acker T, et al. Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis. Nature. 2010;465:487-91 pubmed publisher
Pennisi A, Ling W, Li X, Khan S, Shaughnessy J, Barlogie B, et al. The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth. Blood. 2009;114:1803-12 pubmed publisher
Narazaki G, Uosaki H, Teranishi M, Okita K, Kim B, Matsuoka S, et al. Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells. Circulation. 2008;118:498-506 pubmed publisher
Masuda J, Usui R, Maru Y. Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis. J Biol Chem. 2008;283:13148-55 pubmed publisher
Bouzioukh F, Wilkinson G, Adelmann G, Frotscher M, Stein V, Klein R. Tyrosine phosphorylation sites in ephrinB2 are required for hippocampal long-term potentiation but not long-term depression. J Neurosci. 2007;27:11279-88 pubmed
Parker M, Roberts R, Enriquez M, Zhao X, Takahashi T, Pat Cerretti D, et al. Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway. Biochem Biophys Res Commun. 2004;323:17-23 pubmed
Martiny Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marme D, et al. Inhibition of tumor growth and angiogenesis by soluble EphB4. Neoplasia. 2004;6:248-57 pubmed
Noren N, Lu M, Freeman A, Koolpe M, Pasquale E. Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth. Proc Natl Acad Sci U S A. 2004;101:5583-8 pubmed
Le Noble F, Moyon D, Pardanaud L, Yuan L, Djonov V, Matthijsen R, et al. Flow regulates arterial-venous differentiation in the chick embryo yolk sac. Development. 2004;131:361-75 pubmed
Füller T, Korff T, Kilian A, Dandekar G, Augustin H. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. J Cell Sci. 2003;116:2461-70 pubmed

product information

master code :

446-B4

SKU :

446-B4-200

product name :

Recombinant Mouse EphB4 Fc Chimera Protein, CF

unit size :

200 ug

description :

The Recombinant Mouse EphB4 Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse EphB4 Fc Chimera Protein, CF has been validated for the following applications: Binding Activity.

target :

EphB4

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Mouse

observed molecular weight :

110 kDa, reducing conditions

theoretical molecular weight :

85.2 kDa (monomer)

gene symbol :

Ephb4

details of functionality :

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphB4 Fc Chimera at 2 ╡g/mL (100 ╡L/well) can bind Recombinant Mouse Ephrin-B2 Fc Chimera (Catalog # 496-EB ) with a linear range of 0.078-5 ng/mL. Optimal dilutions should be determined by each laboratory for each application.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

P54761

applications :

Binding Activity

source long :

Mouse myeloma cell line, NS0-derived mouse EphB4 protein Mouse EphB4 (Leu16-Ala539) Accession # P54761 DIEGRMD Human IgG1 (Pro100-Lys330) 6-His tag N-terminus C-terminus

source short :

NS0

USD :

348 USD

alt names :

EC 2.7.10, EC 2.7.10.1, EPH receptor B4, EphB4, ephrin type-B receptor 4, hepatoma transmembrane kinase, Htk, HTKephrin receptor EphB4, Mdk2, Myk1, soluble EPHB4 variant 1, soluble EPHB4 variant 2, soluble EPHB4 variant 3, Tyro11, Tyrosine-protein kinase receptor HTK, Tyrosine-protein kinase TYRO11

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.