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company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse Osteopontin/OPN Protein
catalog :
441-OP-050
quantity :
50 ug (also 200 ug)
price :
305 USD
more info or order :
citations: 40
Reference
Ji J, Zheng S, Liu Y, Xie T, Zhu X, Nie Y, et al. Increased expression of OPN contributes to idiopathic pulmonary fibrosis and indicates a poor prognosis. J Transl Med. 2023;21:640 pubmed publisher
Wang J, Ocadiz Ruiz R, Hall M, Bushnell G, Orbach S, Decker J, et al. A synthetic metastatic niche reveals antitumor neutrophils drive breast cancer metastatic dormancy in the lungs. Nat Commun. 2023;14:4790 pubmed publisher
Wang X, Ramos R, Phan A, Yamaga K, Flesher J, Jiang S, et al. Signalling by senescent melanocytes hyperactivates hair growth. Nature. 2023;618:808-817 pubmed publisher
Andries L, Kancheva D, Masin L, Scheyltjens I, Van Hove H, De Vlaminck K, et al. Immune stimulation recruits a subset of pro-regenerative macrophages to the retina that promotes axonal regrowth of injured neurons. Acta Neuropathol Commun. 2023;11:85 pubmed publisher
Sawaki D, Zhang Y, Mohamadi A, Pini M, Mezdari Z, Lipskaia L, et al. Osteopontin promotes age-related adipose tissue remodeling through senescence-associated macrophage dysfunction. JCI Insight. 2023;8: pubmed publisher
Qiu Y, Shen X, Ravid O, Atrakchi D, Rand D, Wight A, et al. Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease. Proc Natl Acad Sci U S A. 2023;120:e2218915120 pubmed publisher
Bai Q, Wang X, Yan H, Wen L, Zhou Z, Ye Y, et al. Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling. J Pers Med. 2023;13: pubmed publisher
Chen J, Zeng P, Gong L, Zhang X, Ling Z, Bi K, et al. Osteopontin Exacerbates High-Fat Diet-Induced Metabolic Disorders in a Microbiome-Dependent Manner. MBio. 2022;13:e0253122 pubmed publisher
Hong C, Yu I, Pai C, Chen J, Hsieh M, Wu H, et al. CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer. Cancer Res. 2022;82:3734-3750 pubmed publisher
Khamissi F, Ning L, Kefaloyianni E, Dun H, Arthanarisami A, Keller A, et al. Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure. Sci Adv. 2022;8:eabm5900 pubmed publisher
Makishi S, Yamazaki T, Ohshima H. Osteopontin on the Dental Implant Surface Promotes Direct Osteogenesis in Osseointegration. Int J Mol Sci. 2022;23: pubmed publisher
Raineri D, Cappellano G, Vilardo B, Maione F, Clemente N, Canciani E, et al. Inducible T-Cell Costimulator Ligand Plays a Dual Role in Melanoma Metastasis upon Binding to Osteopontin or Inducible T-Cell Costimulator. Biomedicines. 2021;10: pubmed publisher
Yoshida S, Nakagami H, Hayashi H, Ikeda Y, Sun J, Tenma A, et al. The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in mice. Nat Commun. 2020;11:2482 pubmed publisher
Herum K, Romaine A, Wang A, Melleby A, Strand M, Pacheco J, et al. Syndecan-4 Protects the Heart From the Profibrotic Effects of Thrombin-Cleaved Osteopontin. J Am Heart Assoc. 2020;9:e013518 pubmed publisher
Hitscherich P, Xie L, DEL RE D, Lee E. The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models. Physiol Rep. 2019;7:e14137 pubmed publisher
Rogall R, Rabenstein M, Vay S, Bach A, Pikhovych A, Baermann J, et al. Bioluminescence imaging visualizes osteopontin-induced neurogenesis and neuroblast migration in the mouse brain after stroke. Stem Cell Res Ther. 2018;9:182 pubmed publisher
Zeng Q, Luo X, Han M, Liu W, Li H. Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity. EBioMedicine. 2018;32:43-49 pubmed publisher
Natoli R, Yu H, Meislin M, Abbasnia P, ROPER P, Vuchkovska A, et al. Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro. J Orthop Surg Res. 2018;13:101 pubmed publisher
Alvarez M, Xu L, Childress P, Maupin K, Mohamad S, Chitteti B, et al. Megakaryocyte and Osteoblast Interactions Modulate Bone Mass and Hematopoiesis. Stem Cells Dev. 2018;: pubmed publisher
Zhao G, Hu M, Li C, Lee J, Yuan K, Zhu G, et al. Osteopontin contributes to effective neutrophil recruitment, IL-1β production and apoptosis in Aspergillus fumigatus keratitis. Immunol Cell Biol. 2018;96:401-412 pubmed publisher
Quattrocelli M, Capote J, Ohiri J, Warner J, Vo A, Earley J, et al. Genetic modifiers of muscular dystrophy act on sarcolemmal resealing and recovery from injury. PLoS Genet. 2017;13:e1007070 pubmed publisher
Wang W, Li P, Li W, Jiang J, Cui Y, Li S, et al. Osteopontin activates mesenchymal stem cells to repair skin wound. PLoS ONE. 2017;12:e0185346 pubmed publisher
Paul A, Acharya D, Duty L, Thompson E, Le L, Stokic D, et al. Osteopontin facilitates West Nile virus neuroinvasion via neutrophil "Trojan horse" transport. Sci Rep. 2017;7:4722 pubmed publisher
Chen C, Sue Y, Cheng C, Chen Y, Liu C, Hsu Y, et al. Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway. Sci Rep. 2017;7:40183 pubmed publisher
Ciavarra R, Lundberg P, Machida M, Ambrozewicz M, Wellman L, Breving K, et al. Early gene activation initiates neuroinflammation prior to VSV neuroinvasion: Impact on antiviral responses and sleep. J Neuroimmunol. 2017;303:31-42 pubmed publisher
Mellema M, Stoller M, Queau Y, Ho S, Chi T, Larsen J, et al. Nanoparticle Tracking Analysis for the Enumeration and Characterization of Mineralo-Organic Nanoparticles in Feline Urine. PLoS ONE. 2016;11:e0166045 pubmed publisher
Kamioka Y, Takakura K, Sumiyama K, Matsuda M. Intravital Förster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli. Cancer Sci. 2017;108:226-235 pubmed publisher
Sakamoto K, Fukushima Y, Ito K, Matsuda M, Nagata S, Minato N, et al. Osteopontin in Spontaneous Germinal Centers Inhibits Apoptotic Cell Engulfment and Promotes Anti-Nuclear Antibody Production in Lupus-Prone Mice. J Immunol. 2016;197:2177-86 pubmed publisher
Morisaki Y, Niikura M, Watanabe M, Onishi K, Tanabe S, Moriwaki Y, et al. Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9. Sci Rep. 2016;6:27354 pubmed publisher
Maruyama K, Fukasaka M, Uematsu S, Takeuchi O, Kondo T, Saitoh T, et al. 5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival. J Biol Chem. 2015;290:9377-86 pubmed publisher
Qi Q, Xie Q, Liu X, Zhou Y. Osteopontin is expressed in the mouse uterus during early pregnancy and promotes mouse blastocyst attachment and invasion in vitro. PLoS ONE. 2014;9:e104955 pubmed publisher
Kourepini E, Aggelakopoulou M, Alissafi T, Paschalidis N, Simoes D, Panoutsakopoulou V. Osteopontin expression by CD103- dendritic cells drives intestinal inflammation. Proc Natl Acad Sci U S A. 2014;111:E856-65 pubmed publisher
Chen Q, Shou P, Zhang L, Xu C, Zheng C, Han Y, et al. An osteopontin-integrin interaction plays a critical role in directing adipogenesis and osteogenesis by mesenchymal stem cells. Stem Cells. 2014;32:327-37 pubmed publisher
Shojaei F, Scott N, Kang X, Lappin P, Fitzgerald A, Karlicek S, et al. Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer. J Exp Clin Cancer Res. 2012;31:26 pubmed publisher
Dahiya S, Givvimani S, Bhatnagar S, Qipshidze N, Tyagi S, Kumar A. Osteopontin-stimulated expression of matrix metalloproteinase-9 causes cardiomyopathy in the mdx model of Duchenne muscular dystrophy. J Immunol. 2011;187:2723-31 pubmed publisher
Schneider D, Lindsay J, Zhou Y, Molina J, Blackburn M. Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease. FASEB J. 2010;24:70-80 pubmed publisher
Marcondes M, Poling M, Watry D, Hall D, Fox H. In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression. Cell Immunol. 2008;254:56-62 pubmed publisher
Xanthou G, Alissafi T, Semitekolou M, Simoes D, Economidou E, Gaga M, et al. Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets. Nat Med. 2007;13:570-8 pubmed
Jacobs J, Pettit A, Shinohara M, Jansson M, Cantor H, Gravallese E, et al. Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis. Arthritis Rheum. 2004;50:2685-94 pubmed
Bruemmer D, Collins A, Noh G, Wang W, Territo M, Arias Magallona S, et al. Angiotensin II-accelerated atherosclerosis and aneurysm formation is attenuated in osteopontin-deficient mice. J Clin Invest. 2003;112:1318-31 pubmed
product information
brand :
R&D Systems, a Bio-Techne Brand
catalog number base :
441-OP
SKU :
441-OP-050
product name :
Recombinant Mouse Osteopontin/OPN Protein
Description :
The Recombinant Mouse Osteopontin/OPN Protein from R&D Systems is derived from NS0. The Recombinant Mouse Osteopontin/OPN Protein has been validated for the following applications: Bioactivity.
target :
Osteopontin/OPN
category :
Proteins and Enzymes
unit size :
50 ug (also 200 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Mouse
observed molecular weight :
30 kDa and a 65 kDa doublet, reducing conditions
theoretical molecular weight :
31.5 kDa
gene symbol :
Spp1
details of functionality :
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al . (2001) J. Biol. Chem. 276 :28261. When 1 x 105 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is 0.25-1.5 µg/mL. Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE ) proteolytic treatment of this rmOsteopontin can increase HEK293 cell adhesion by about 10-fold.
endotoxin note :
<0.01 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
Q547B5
applications :
Bioactivity
source :
NS0-derived Recombinant Mouse Osteopontin/OPN Protein
USD :
305 USD
alt names :
BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, osteopontin, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), Spp1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, uropontin
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.