product summary
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company name :
R&D Systems
product type :
antibody
product name :
PAR/pADPr Antibody
catalog :
4335-MC-100
quantity :
100 uL
price :
409 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
10HA
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, proximity ligation assay
more info or order :
citations: 65
Reference
Alirzayeva H, Loureiro R, Koyuncu S, Hommen F, Nabawi Y, Zhang W, et al. ALS-FUS mutations cause abnormal PARylation and histone H1.2 interaction, leading to pathological changes. Cell Rep. 2024;43:114626 pubmed publisher
Chudy P, Kochan J, Wawro M, Nguyen P, Gorczyca M, Varanko A, et al. Heme oxygenase-1 protects cells from replication stress. Redox Biol. 2024;75:103247 pubmed publisher
Shu Y, Jin X, Ji M, Zhang Z, Wang X, Liang H, et al. Ku70 Binding to YAP Alters PARP1 Ubiquitination to Regulate Genome Stability and Tumorigenesis. Cancer Res. 2024;84:2836-2855 pubmed publisher
Saha S, Huang S, Yang X, Saha L, Sun Y, Khandagale P, et al. The TDRD3-USP9X complex and MIB1 regulate TOP3B homeostasis and prevent deleterious TOP3B cleavage complexes. Nat Commun. 2023;14:7524 pubmed publisher
Wu A, Sekar P, Huang D, Hsu S, Chan C, Lin W. Spatiotemporal roles of AMPK in PARP-1- and autophagy-dependent retinal pigment epithelial cell death caused by UVA. J Biomed Sci. 2023;30:91 pubmed publisher
Maestri D, Napoletani G, Kossenkov A, Preston Alp S, Caruso L, Tempera I. The three-dimensional structure of the EBV genome plays a crucial role in regulating viral gene expression in EBVaGC. Nucleic Acids Res. 2023;51:12092-12110 pubmed publisher
Li P, Zhen Y, Kim C, Liu Z, Hao J, Deng H, et al. Nimbolide targets RNF114 to induce the trapping of PARP1 and synthetic lethality in BRCA-mutated cancer. Sci Adv. 2023;9:eadg7752 pubmed publisher
Li J, Liu X, Peng B, Feng T, Zhou W, Meng L, et al. O-GlcNAc has crosstalk with ADP-ribosylation via PARG. J Biol Chem. 2023;299:105354 pubmed publisher
Zhu Y, Zhang J, Yan X, Ji Y, Wang F. Exploring a new mechanism between lactate and VSMC calcification: PARP1/POLG/UCP2 signaling pathway and imbalance of mitochondrial homeostasis. Cell Death Dis. 2023;14:598 pubmed publisher
Chen W, E Q, Sun B, Zhang P, Li N, Fei S, et al. PARP1-catalyzed PARylation of YY1 mediates endoplasmic reticulum stress in granulosa cells to determine primordial follicle activation. Cell Death Dis. 2023;14:524 pubmed publisher
Wie M, Khim K, Groehler Iv A, Heo S, Woo J, Son K, et al. Alkylation of nucleobases by 2-chloro-N,N-diethylethanamine hydrochloride (CDEAH) sensitizes PARP1-deficient tumors. NAR Cancer. 2023;5:zcad042 pubmed publisher
Yang S, Hwang S, Kim B, Shin S, Kim M, Jeong S. Fatty acid oxidation facilitates DNA double-strand break repair by promoting PARP1 acetylation. Cell Death Dis. 2023;14:435 pubmed publisher
Ramos L, Truong S, Zhai B, Joshi J, Ghaidi F, Lizardo M, et al. A Bifunctional PARP-HDAC Inhibitor with Activity in Ewing Sarcoma. Clin Cancer Res. 2023;29:3541-3553 pubmed publisher
Han C, McNamara B, Bellone S, Harold J, Manara P, Hartwich T, et al. The Poly (ADP-ribose) polymerase inhibitor olaparib and pan-ErbB inhibitor neratinib are highly synergistic in HER2 overexpressing epithelial ovarian carcinoma in vitro and in vivo. Gynecol Oncol. 2023;170:172-178 pubmed publisher
Qi H, Grace Wright R, Beato M, Price B. The ADP-ribose hydrolase NUDT5 is important for DNA repair. Cell Rep. 2022;41:111866 pubmed publisher
Wang L, Wang D, Sonzogni O, Ke S, Wang Q, Thavamani A, et al. PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Cell Rep. 2022;41:111462 pubmed publisher
Abdelghany L, Kawabata T, Goto S, Jingu K, Li T. Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells. Transl Oncol. 2022;26:101548 pubmed publisher
Deng O, Dash S, Nepomuceno T, Fang B, Yun S, Welsh E, et al. Integrated proteomics identifies PARP inhibitor-induced prosurvival signaling changes as potential vulnerabilities in ovarian cancer. J Biol Chem. 2022;298:102550 pubmed publisher
Thakar T, Dhoonmoon A, Straka J, Schleicher E, Nicolae C, Moldovan G. Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling. Nat Commun. 2022;13:5323 pubmed publisher
Cahuzac M, P xe9 ant B, Mes Masson A, Saad F. Development of Olaparib-Resistance Prostate Cancer Cell Lines to Identify Mechanisms Associated with Acquired Resistance. Cancers (Basel). 2022;14: pubmed publisher
Wong W, Crane E, Zhang H, Li J, Day T, Green A, et al. Pgc-1α controls epidermal stem cell fate and skin repair by sustaining NAD+ homeostasis during aging. Mol Metab. 2022;65:101575 pubmed publisher
Blessing C, Apelt K, van den Heuvel D, González Leal C, Rother M, van der Woude M, et al. XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair. Nat Commun. 2022;13:4762 pubmed publisher
Zhao S, Hong Y, Liang Y, Li X, Shen J, Sun C, et al. Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast. Redox Biol. 2022;55:102414 pubmed publisher
Zhang Y, Fang M, Li S, Xu H, Ren J, Tu L, et al. BTApep-TAT peptide inhibits ADP-ribosylation of BORIS to induce DNA damage in cancer. Mol Cancer. 2022;21:158 pubmed publisher
Trulsson F, Akimov V, Robu M, van Overbeek N, Berrocal D, Shah R, et al. Deubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates. Nat Commun. 2022;13:2736 pubmed publisher
Linares J, Cid Díaz T, Duran A, Osrodek M, Martínez Ordóñez A, Reina Campos M, et al. The lactate-NAD+ axis activates cancer-associated fibroblasts by downregulating p62. Cell Rep. 2022;39:110792 pubmed publisher
Liu L, Sandow J, Leslie Pedrioli D, Samson A, Silke N, Kratina T, et al. Tankyrase-mediated ADP-ribosylation is a regulator of TNF-induced death. Sci Adv. 2022;8:eabh2332 pubmed publisher
Lin X, Jiang W, Rudolph J, Lee B, Luger K, Zha S. PARP inhibitors trap PARP2 and alter the mode of recruitment of PARP2 at DNA damage sites. Nucleic Acids Res. 2022;50:3958-3973 pubmed publisher
Cahuzac M, Langlois P, P xe9 ant B, Fleury H, Mes Masson A, Saad F. Pre-activation of autophagy impacts response to olaparib in prostate cancer cells. Commun Biol. 2022;5:251 pubmed publisher
Hou X, Cai C, He Y, An S, Zhao S, Sun H, et al. Protective Effect of Minocycline Hydrochloride on the Mouse Embryonic Development Against Suboptimal Environment. Front Cell Dev Biol. 2022;10:799042 pubmed publisher
Chen Q, Ma K, Liu X, Chen S, Li P, Yu Y, et al. Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis. Cell Discov. 2022;8:3 pubmed publisher
Sethy C, Kundu C. PARP inhibitor BMN-673 induced apoptosis by trapping PARP-1 and inhibiting base excision repair via modulation of pol-β in chromatin of breast cancer cells. Toxicol Appl Pharmacol. 2022;436:115860 pubmed publisher
Sarwar Z, Nabi N, Bhat S, Gillani S, Reshi I, Un Nisa M, et al. Interaction of DBC1 with polyoma small T antigen promotes its degradation and negatively regulates tumorigenesis. J Biol Chem. 2022;298:101496 pubmed publisher
Cooper K, Volk L, Dominguez D, Duran A, Ke Jian Liu K, Hudson L. Contribution of NADPH oxidase to the retention of UVR-induced DNA damage by arsenic. Toxicol Appl Pharmacol. 2022;434:115799 pubmed publisher
Park S, Kim Y, Ra J, Wie M, Kang M, Kang S, et al. Timely termination of repair DNA synthesis by ATAD5 is important in oxidative DNA damage-induced single-strand break repair. Nucleic Acids Res. 2021;49:11746-11764 pubmed publisher
Tromans Coia C, Sanchi A, Moeller G, Timinszky G, Lopes M, Ahel I. TARG1 protects against toxic DNA ADP-ribosylation. Nucleic Acids Res. 2021;49:10477-10492 pubmed publisher
Sun Y, Chen J, Huang S, Su Y, Wang W, Agama K, et al. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation. Nat Commun. 2021;12:5010 pubmed publisher
Peng B, Shi R, Bian J, Li Y, Wang P, Wang H, et al. PARP1 and CHK1 coordinate PLK1 enzymatic activity during the DNA damage response to promote homologous recombination-mediated repair. Nucleic Acids Res. 2021;49:7554-7570 pubmed publisher
Oka S, Leon J, Sakumi K, Abolhassani N, Sheng Z, Tsuchimoto D, et al. MTH1 and OGG1 maintain a low level of 8-oxoguanine in Alzheimer's brain, and prevent the progression of Alzheimer's pathogenesis. Sci Rep. 2021;11:5819 pubmed publisher
Martí J, García Díaz A, Delgado Bellido D, O Valle F, González Flores A, Carlevaris O, et al. Selective modulation by PARP-1 of HIF-1α-recruitment to chromatin during hypoxia is required for tumor adaptation to hypoxic conditions. Redox Biol. 2021;41:101885 pubmed publisher
Chung W, Lee S, Kim Y, Seo J, Song M. Kaposi's sarcoma-associated herpesvirus processivity factor (PF-8) recruits cellular E3 ubiquitin ligase CHFR to promote PARP1 degradation and lytic replication. PLoS Pathog. 2021;17:e1009261 pubmed publisher
Matteini F, Andresini O, Petrai S, Battistelli C, Rossi M, Maione R. Poly(ADP-ribose) Polymerase 1 (PARP1) restrains MyoD-dependent gene expression during muscle differentiation. Sci Rep. 2020;10:15086 pubmed publisher
Gibson A, Yeung C, Issaq S, Collins V, Gouzoulis M, Zhang Y, et al. Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma. Oncogenesis. 2020;9:80 pubmed publisher
Shao Z, Lee B, Rouleau Turcotte É, Langelier M, Lin X, Estes V, et al. Clinical PARP inhibitors do not abrogate PARP1 exchange at DNA damage sites in vivo. Nucleic Acids Res. 2020;48:9694-9709 pubmed publisher
Patidar P, Viera T, Morales J, Singh N, Motea E, Khandelwal M, et al. XRN2 interactome reveals its synthetic lethal relationship with PARP1 inhibition. Sci Rep. 2020;10:14253 pubmed publisher
Hanzlikova H, Prokhorova E, Krejcikova K, Cihlarova Z, Kalasová I, Kubovčiak J, et al. Pathogenic ARH3 mutations result in ADP-ribose chromatin scars during DNA strand break repair. Nat Commun. 2020;11:3391 pubmed publisher
Mao K, Chen J, Yu H, Li H, Ren Y, Wu X, et al. Poly (ADP-ribose) polymerase 1 inhibition prevents neurodegeneration and promotes α-synuclein degradation via transcription factor EB-dependent autophagy in mutant α-synucleinA53T model of Parkinson's disease. Aging Cell. 2020;19:e13163 pubmed publisher
Calahorra J, Martinez Lara E, Granadino Roldán J, Martí J, Cañuelo A, Blanco S, et al. Crosstalk between hydroxytyrosol, a major olive oil phenol, and HIF-1 in MCF-7 breast cancer cells. Sci Rep. 2020;10:6361 pubmed publisher
Yang G, Chen Y, Wu J, Chen S, Liu X, Singh A, et al. Poly(ADP-ribosyl)ation mediates early phase histone eviction at DNA lesions. Nucleic Acids Res. 2020;48:3001-3013 pubmed publisher
Zhang L, Li D. MORC2 regulates DNA damage response through a PARP1-dependent pathway. Nucleic Acids Res. 2019;47:8502-8520 pubmed publisher
Li N, Wang Y, Neri S, Zhen Y, Fong L, Qiao Y, et al. Tankyrase disrupts metabolic homeostasis and promotes tumorigenesis by inhibiting LKB1-AMPK signalling. Nat Commun. 2019;10:4363 pubmed publisher
Chen Y, Zhang H, Xu Z, Tang H, Geng A, Cai B, et al. A PARP1-BRG1-SIRT1 axis promotes HR repair by reducing nucleosome density at DNA damage sites. Nucleic Acids Res. 2019;47:8563-8580 pubmed publisher
Jain A, Agostini L, McCarthy G, Chand S, Ramirez A, Nevler A, et al. Poly (ADP) ribose glycohydrolase can be effectively targeted in pancreatic cancer. Cancer Res. 2019;: pubmed publisher
Marcar L, Bardhan K, Gheorghiu L, Dinkelborg P, Pfäffle H, Liu Q, et al. Acquired Resistance of EGFR-Mutated Lung Cancer to Tyrosine Kinase Inhibitor Treatment Promotes PARP Inhibitor Sensitivity. Cell Rep. 2019;27:3422-3432.e4 pubmed publisher
Echeverri Tirado L, Ghonim M, Wang J, Al Khami A, Wyczechowska D, Luu H, et al. PARP-1 Is Critical for Recruitment of Dendritic Cells to the Lung in a Mouse Model of Asthma but Dispensable for Their Differentiation and Function. Mediators Inflamm. 2019;2019:1656484 pubmed publisher
Grunewald M, Chen Y, Kuny C, Maejima T, Lease R, Ferraris D, et al. The coronavirus macrodomain is required to prevent PARP-mediated inhibition of virus replication and enhancement of IFN expression. PLoS Pathog. 2019;15:e1007756 pubmed publisher
Lombard A, Liu C, Armstrong C, D Abronzo L, Lou W, Chen H, et al. Overexpressed ABCB1 Induces Olaparib-Taxane Cross-Resistance in Advanced Prostate Cancer. Transl Oncol. 2019;12:871-878 pubmed publisher
Wang C, Xu W, An J, Liang M, Li Y, Zhang F, et al. Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2. Nat Commun. 2019;10:1203 pubmed publisher
Bian C, Zhang C, Luo T, Vyas A, Chen S, Liu C, et al. NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression. Nat Commun. 2019;10:693 pubmed publisher
Li M, Tang Y, Li Q, Xiao M, Yang Y, Wang Y. Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2. Oncogene. 2019;38:3488-3503 pubmed publisher
Nieborowska Skorska M, Sullivan K, Dasgupta Y, Podszywalow Bartnicka P, Hoser G, Maifrede S, et al. Gene expression and mutation-guided synthetic lethality eradicates proliferating and quiescent leukemia cells. J Clin Invest. 2017;127:2392-2406 pubmed publisher
Kulkarni A, Oza J, Yao M, Sohail H, Ginjala V, Tomás Loba A, et al. Tripartite Motif-containing 33 (TRIM33) protein functions in the poly(ADP-ribose) polymerase (PARP)-dependent DNA damage response through interaction with Amplified in Liver Cancer 1 (ALC1) protein. J Biol Chem. 2013;288:32357-69 pubmed publisher
Fenton A, Shirodkar P, Macrae C, Meng L, Koch C. The PARP3- and ATM-dependent phosphorylation of APLF facilitates DNA double-strand break repair. Nucleic Acids Res. 2013;41:4080-92 pubmed publisher
Yan Q, Xu R, Zhu L, Cheng X, Wang Z, Manis J, et al. BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8. Mol Cell Biol. 2013;33:845-57 pubmed publisher
Jaspers J, Kersbergen A, Boon U, Sol W, Van Deemter L, Zander S, et al. Loss of 53BP1 causes PARP inhibitor resistance in Brca1-mutated mouse mammary tumors. Cancer Discov. 2013;3:68-81 pubmed publisher
product information
brand :
R&D Systems
master code :
4335-MC-100
SKU :
4335-MC-100
product name :
PAR/pADPr Antibody
unit size :
100 uL
seo description :
The PAR/pADPr Antibody from R&D Systems is a mouse monoclonal antibody to PAR/pADPr. This antibody reacts with human,mouse,multi-species,n/a,rat,transgenic mouse. The PAR/pADPr Antibody has been validated for the following applications: Western Blot,Immunoprecipitation,Immunocytochemistry,Western Blot Control,Immunohistochemistry,Co-Immunoprecipitation,Westen Blot,Flow Cytometry,Proximity Ligation Assay,Functional Assay,ELISA Capture,Immunohistochemistry-Paraffin,Chromatin Immunoprecipitation (ChIP),Intracellular Staining by Flow Cytometry.
target :
PAR/pADPr
category :
Primary Antibodies
buffer :
Supplied as 100 ╡L of a 0.2 ╡m filtered solution in TBS, at a concentration of 1 mg/mL
clonality :
Monoclonal
clone :
10HA
conjugate :
Unconjugated
dilution :
Western Blot 1:1000 dilution, ELISA, Immunohistochemistry 1-15 ug/mL, Immunoprecipitation, Immunoaffinity Purification, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, Immunocytochemistry 3-25 ug/mL
host :
Mouse
immunogen :
Purified ADP-ribose polymers between 2 and 50 units long
isotype :
IgG3
purity :
Protein A or G purified from ascites
species :
Human,Mouse,Multi-Species,N/A,Rat,Transgenic Mouse
specificity :
The antibody is specific for PAR polymers 2 to 50 units long, but does not recognize structurally related RNA, DNA, ADP-ribose monomers, NAD, or other nucleic acid monomers. Detects Poly(ADP-ribose) Polymer in Direct ELISA.
applications :
Intracellular Staining by Flow Cytometry,Co-Immunoprecipitation,Western Blot Control,Westen Blot,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Proximity Ligation Assay,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,Functional Assay,ELISA Capture
USD :
409 USD
alt names :
PAR, Poly(ADP-ribose)
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C, as supplied. 1 month, 2 to 8 ░C under sterile conditions after opening. 6 months, -20 to -70 ░C under sterile conditions after opening.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.