Recombinant Human Fc gamma RIIIA/CD16a Protein, CF | R&D Systems 4325-FC product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

catalog :

4325-FC

quantity :

50 ug

price :

349 USD

citations: 14

Reference
Blundell P, Lu D, Wilkinson M, Dell A, Haslam S, Pleass R. Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus. J Immunol. 2019;202:1595-1611 pubmed publisher
Wozniak Knopp G, Stadlmayr G, Perthold J, Stadlbauer K, Gotsmy M, Becker S, et al. An antibody with Fab-constant domains exchanged for a pair of CH3 domains. PLoS ONE. 2018;13:e0195442 pubmed publisher
Arce Vargas F, Furness A, Litchfield K, Joshi K, Rosenthal R, Ghorani E, et al. Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies. Cancer Cell. 2018;33:649-663.e4 pubmed publisher
Lee C, Jeong M, Lee J, Seo S, Cho S, Zhang W, et al. Glycosylation profile and biological activity of Remicade® compared with Flixabi® and Remsima®. MAbs. 2017;9:968-977 pubmed publisher
Le Clorennec C, BAZIN H, Dubreuil O, Larbouret C, Ogier C, Lazrek Y, et al. Neuregulin 1 Allosterically Enhances the Antitumor Effects of the Noncompeting Anti-HER3 Antibody 9F7-F11 by Increasing Its Binding to HER3. Mol Cancer Ther. 2017;16:1312-1323 pubmed publisher
Lohse S, Meyer S, Meulenbroek L, Jansen J, Nederend M, Kretschmer A, et al. An Anti-EGFR IgA That Displays Improved Pharmacokinetics and Myeloid Effector Cell Engagement In Vivo. Cancer Res. 2016;76:403-17 pubmed publisher
Kinder M, Greenplate A, Strohl W, Jordan R, Brezski R. An Fc engineering approach that modulates antibody-dependent cytokine release without altering cell-killing functions. MAbs. 2015;7:494-504 pubmed publisher
Yang Z, Ramsey J, Hamza T, Zhang Y, Li S, Yfantis H, et al. Mechanisms of protection against Clostridium difficile infection by the monoclonal antitoxin antibodies actoxumab and bezlotoxumab. Infect Immun. 2015;83:822-31 pubmed publisher
Anikeeva N, Steblyanko M, Fayngerts S, Kopylova N, Marshall D, Powers G, et al. Integrin receptors on tumor cells facilitate NK cell-mediated antibody-dependent cytotoxicity. Eur J Immunol. 2014;44:2331-9 pubmed publisher
Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex Rabe S, et al. Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases. J Immunol. 2014;192:4083-92 pubmed publisher
Lajoie L, Congy Jolivet N, Bolzec A, Gouilleux Gruart V, Sicard E, Sung H, et al. ADAM17-mediated shedding of Fc?RIIIA on human NK cells: identification of the cleavage site and relationship with activation. J Immunol. 2014;192:741-51 pubmed publisher
Boltz A, Piater B, Toleikis L, Guenther R, Kolmar H, Hock B. Bi-specific aptamers mediating tumor cell lysis. J Biol Chem. 2011;286:21896-905 pubmed publisher
von Horsten H, Ogorek C, Blanchard V, Demmler C, Giese C, Winkler K, et al. Production of non-fucosylated antibodies by co-expression of heterologous GDP-6-deoxy-D-lyxo-4-hexulose reductase. Glycobiology. 2010;20:1607-18 pubmed publisher
Wei Y, Li C, Huang W, Li B, Strome S, Wang L. Glycoengineering of human IgG1-Fc through combined yeast expression and in vitro chemoenzymatic glycosylation. Biochemistry. 2008;47:10294-304 pubmed publisher

product information

brand :

R&D Systems

master code :

4325-FC

SKU :

4325-FC-050

product name :

Recombinant Human Fc gamma RIIIA/CD16a Protein, CF

description :

The Recombinant Human Fc gamma RIIIA/CD16a Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Fc gamma RIIIA/CD16a Protein, CF has been validated for the following applications: Binding Activity.

target :

Fc gamma RIIIA/CD16a

category :

Proteins and Enzymes

unit size :

50 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

40-50 kDa, reducing conditions

theoretical molecular weight :

22.6 kDa

gene symbol :

FCGR3A

details of functionality :

Measured by its ability to bind human IgG with an estimated Kd 50 nM.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

catalog number base :

4325-FC

accessionNumbers :

AAH17865

applications :

Binding Activity

source :

Mouse myeloma cell line, NS0-derived human Fc gamma RIIIA/CD16a protein Gly17-Gln208, with a C-terminal 6-His tag

extended description :

Isoform V176

2020 USD :

349

2021 USD :

349 USD

product details :

Isoform V176

alt names :

CD16a, CD16a antigen, CD16FCRIIIA, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for (CD16), Fc gamma receptor III-A, FCG3, Fc-gamma receptor III-2 (CD 16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIIIa, Fc-gamma RIII-alpha, FCGR3, FCGR3A, FCGRIII, FcgRIIIA, FCR-10, FcRIII, FcRIIIa, IGFR3FcgammaRIIIA, immunoglobulin G Fc receptor III, low affinity III, receptor for (CD16), low affinity immunoglobulin gamma Fc region receptor III-A, neutrophil-specific antigen NA

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.