Recombinant Human Fibronectin Protein, CF | R&D Systems 4305-FNB-200 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Fibronectin Protein, CF

catalog :

4305-FNB-200

quantity :

200 ug

price :

430 USD

more info or order :

R&D Systems product webpage

citations: 8

Reference
Soldati S, B xe4 r A, Vladymyrov M, Glavin D, McGrath J, Gosselet F, et al. High levels of endothelial ICAM-1 prohibit natalizumab mediated abrogation of CD4+ T cell arrest on the inflamed BBB under flow in vitro. J Neuroinflammation. 2023;20:123 pubmed publisher
Bluhmki T, Traub S, Müller A, Bitzer S, Schruf E, Bammert M, et al. Functional human iPSC-derived alveolar-like cells cultured in a miniaturized 96‑Transwell air-liquid interface model. Sci Rep. 2021;11:17028 pubmed publisher
Koh K, Cao Y, Mangos S, Tardi N, Dande R, Lee H, et al. Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist. J Clin Invest. 2019;129:1713-1726 pubmed publisher
Frémin C, Guegan J, Plutoni C, Mahaffey J, Philips M, Emery G, et al. ERK1/2-induced phosphorylation of R-Ras GTPases stimulates their oncogenic potential. Oncogene. 2016;35:5692-5698 pubmed publisher
Jeon M, Lee J, Nam S, Shin I, Lee J, Kim S. Induction of fibronectin by HER2 overexpression triggers adhesion and invasion of breast cancer cells. Exp Cell Res. 2015;333:116-26 pubmed publisher
Ayala Lujan J, Vijayakumar V, Gong M, Smith R, Santiago A, Ruiz Perez F. Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes. PLoS ONE. 2014;9:e107920 pubmed publisher
Dodd K, Yang J, Shen M, Sampson J, Tee A. mTORC1 drives HIF-1? and VEGF-A signalling via multiple mechanisms involving 4E-BP1, S6K1 and STAT3. Oncogene. 2015;34:2239-50 pubmed publisher
Chang M, Huang D, Ho F, Huang K, Lin W. PKC-dependent human monocyte adhesion requires AMPK and Syk activation. PLoS ONE. 2012;7:e40999 pubmed publisher

product information

brand :

R&D Systems, a Bio-Techne brand

catalog number base :

4305-FNB

SKU :

4305-FNB-200

product name :

Recombinant Human Fibronectin Protein, CF

description :

The Recombinant Human Fibronectin Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Fibronectin Protein, CF has been validated for the following applications: Bioactivity.

target :

Fibronectin

unit size :

200 ug

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 µm filtered solution in HEPES, NaCl and Tween®.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.

observed molecular weight :

200-250 kDa, reducing conditions

theoretical molecular weight :

207 kDa

details of functionality :

Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is typically 1-5 µg/cm2. Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50 µg/mL. Optimal concentrations will need to be determined for individual user applications .

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

CAE45932

applications :

Bioactivity

product keywords :

4305-FN, rhFibronectin, hFibronectin, CF, carrier-free, carrier free, 43O5-FNB, 4305FNB, Integrin, Protease Substrate, FN-1.1, FN-1.2, FN-1.3, FN-1.4, Fibronectin 1, Cold-Insoluble Globulin, Migration-Stimulating Factor, CIG, Epididymis Secretory Sperm Binding Protein, GFND2, SMDCF, ED-B, FINC, GFND, LETS, FNZ, MSF

source :

NS0-derived Recombinant Human Fibronectin Protein, CF

USD :

425

USD 2025 :

430 USD

alt names :

CIG, ED-B, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF, SMDCF

more info or order :

R&D Systems product webpage