product summary
Loading...
company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse IL-10 Protein
catalog :
417-ML-005
quantity :
5 ug (also 25 ug, 100 ug, 250 ug)
price :
286 USD
more info or order :
citations: 71
Reference
Hou Y, Wei D, Zhang Z, Lei T, Li S, Bao J, et al. Downregulation of nutrition sensor GCN2 in macrophages contributes to poor wound healing in diabetes. Cell Rep. 2024;43:113658 pubmed publisher
Adrian M, Weber M, Tsai M, Glock C, Kahn O, Phu L, et al. Polarized microtubule remodeling transforms the morphology of reactive microglia and drives cytokine release. Nat Commun. 2023;14:6322 pubmed publisher
Santiago J, Natu A, Ramelow C, Rayaprolu S, Xiao H, Kumar V, et al. Identification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles. bioRxiv. 2023;: pubmed publisher
Yeung S, Ovando L, Russo A, Rathinam V, Khanna K. CD169+ macrophage intrinsic IL-10 production regulates immune homeostasis during sepsis. Cell Rep. 2023;42:112171 pubmed publisher
Shaik A, Shaik A, Abudawood M, Al Faraj A. Detection of Radiolabeled Inflammatory Cell Macrophage Subpopulations in Chronic Respiratory Diseases: Results from Preliminary Analyses. Biomed Res Int. 2022;2022:9470845 pubmed publisher
Qiu M, Shu H, Li L, Shen Y, Tian Y, Ji Y, et al. Interleukin 10 Attenuates Angiotensin II-Induced Aortic Remodelling by Inhibiting Oxidative Stress-Induced Activation of the Vascular p38 and NF-κB Pathways. Oxid Med Cell Longev. 2022;2022:8244497 pubmed publisher
Liang Z, He P, Han Y, Yun C. Survival of Stem Cells and Progenitors in the Intestine Is Regulated by LPA5-Dependent Signaling. Cell Mol Gastroenterol Hepatol. 2022;14:129-150 pubmed publisher
Yogev N, Bedke T, Kobayashi Y, Brockmann L, Lukas D, Regen T, et al. CD4+ T-cell-derived IL-10 promotes CNS inflammation in mice by sustaining effector T cell survival. Cell Rep. 2022;38:110565 pubmed publisher
Yamazaki T, Yamada S, Ohshio K, Sugamata M, Morita Y. Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages. Int J Mol Sci. 2022;23: pubmed publisher
Chauhan P, Sheng W, Hu S, Prasad S, Lokensgard J. Differential Cytokine-Induced Responses of Polarized Microglia. Brain Sci. 2021;11: pubmed publisher
Shi Y, Li S, Zhang H, Zhu J, Che T, Yan B, et al. The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons. J Neuroinflammation. 2021;18:261 pubmed publisher
Dupraz L, Magniez A, Rolhion N, Richard M, Da Costa G, Touch S, et al. Gut microbiota-derived short-chain fatty acids regulate IL-17 production by mouse and human intestinal γδ T cells. Cell Rep. 2021;36:109332 pubmed publisher
Dowling J, Afzal R, Gearing L, Cervantes Silva M, Annett S, Davis G, et al. Mitochondrial arginase-2 is essential for IL-10 metabolic reprogramming of inflammatory macrophages. Nat Commun. 2021;12:1460 pubmed publisher
Xie K, Chai Y, Lin S, Xu F, Wang C. Luteolin Regulates the Differentiation of Regulatory T Cells and Activates IL-10-Dependent Macrophage Polarization against Acute Lung Injury. J Immunol Res. 2021;2021:8883962 pubmed publisher
Sanmarco L, Wheeler M, Gutiérrez Vázquez C, Polonio C, Linnerbauer M, Pinho Ribeiro F, et al. Gut-licensed IFNγ+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes. Nature. 2021;: pubmed publisher
Rangasami V, Samanta S, Parihar V, Asawa K, Zhu K, Varghese O, et al. Harnessing hyaluronic acid-based nanoparticles for combination therapy: A novel approach for suppressing systemic inflammation and to promote antitumor macrophage polarization. Carbohydr Polym. 2021;254:117291 pubmed publisher
Yamazaki T, Ohshio K, Sugamata M, Morita Y. Lactic acid bacterium, Lactobacillus paracasei KW3110, suppresses inflammatory stress-induced caspase-1 activation by promoting interleukin-10 production in mouse and human immune cells. PLoS ONE. 2020;15:e0237754 pubmed publisher
Luiz J, Toller Kawahisa J, Viacava P, Nascimento D, Pereira P, Saraiva A, et al. MEK5/ERK5 signaling mediates IL-4-induced M2 macrophage differentiation through regulation of c-Myc expression. J Leukoc Biol. 2020;108:1215-1223 pubmed publisher
Chen Y, Wang C, Xie K, Lei M, Chai Y, Xu F, et al. Progranulin Improves Acute Lung Injury through Regulating the Differentiation of Regulatory T Cells and Interleukin-10 Immunomodulation to Promote Macrophage Polarization. Mediators Inflamm. 2020;2020:9704327 pubmed publisher
Nelson A, Maddaloni M, Abbott J, Hoffman C, Akgul A, Ohland C, et al. Oral therapy with colonization factor antigen I prevents development of type 1 diabetes in Non-obese Diabetic mice. Sci Rep. 2020;10:6156 pubmed publisher
Muldoon J, Chuang Y, Bagheri N, Leonard J. Macrophages employ quorum licensing to regulate collective activation. Nat Commun. 2020;11:878 pubmed publisher
Kim M, Yang J, Ko Y, Lee H, Oh S, Cho W, et al. Impact of aging on transition of acute kidney injury to chronic kidney disease. Sci Rep. 2019;9:18445 pubmed publisher
Ondee T, Gillen J, Visitchanakun P, Somparn P, Issara Amphorn J, Dang Phi C, et al. Lipocalin-2 (Lcn-2) Attenuates Polymicrobial Sepsis with LPS Preconditioning (LPS Tolerance) in FcGRIIb Deficient Lupus Mice. Cells. 2019;8: pubmed publisher
Ciesielska A, Hromada Judycka A, Ziemlinska E, Kwiatkowska K. Lysophosphatidic acid up-regulates IL-10 production to inhibit TNF-α synthesis in Mϕs stimulated with LPS. J Leukoc Biol. 2019;106:1285-1301 pubmed publisher
Hayek I, Fischer F, Schulze Luehrmann J, Dettmer K, Sobotta K, Schatz V, et al. Limitation of TCA Cycle Intermediates Represents an Oxygen-Independent Nutritional Antibacterial Effector Mechanism of Macrophages. Cell Rep. 2019;26:3502-3510.e6 pubmed publisher
Paduch K, Debus A, Rai B, Schleicher U, Bogdan C. Resolution of Cutaneous Leishmaniasis and Persistence of Leishmania major in the Absence of Arginase 1. J Immunol. 2019;202:1453-1464 pubmed publisher
Forteza M, Polyzos K, Baumgartner R, Suur B, Mussbacher M, Johansson D, et al. Activation of the Regulatory T-Cell/Indoleamine 2,3-Dioxygenase Axis Reduces Vascular Inflammation and Atherosclerosis in Hyperlipidemic Mice. Front Immunol. 2018;9:950 pubmed publisher
Rangaraju S, Raza S, Li N, Betarbet R, Dammer E, Duong D, et al. Differential Phagocytic Properties of CD45low Microglia and CD45high Brain Mononuclear Phagocytes-Activation and Age-Related Effects. Front Immunol. 2018;9:405 pubmed publisher
Bröker K, Figge J, Magnusen A, Manz R, Köhl J, Karsten C. A Novel Role for C5a in B-1 Cell Homeostasis. Front Immunol. 2018;9:258 pubmed publisher
Kanai K, Park A, Watanabe A, Arikawa T, Yasui T, Yoshida H, et al. Murine γ-Herpesvirus 68 Induces Severe Lung Inflammation in IL-27-Deficient Mice with Liver Dysfunction Preventable by Oral Neomycin. J Immunol. 2018;200:2703-2713 pubmed publisher
St John A, Ang G, Rathore A, Abraham S. Reprograming Immunity to Food Allergens. J Allergy Clin Immunol. 2018;: pubmed publisher
Vadevoo S, Kim J, Gunassekaran G, Jung H, Chi L, Kim D, et al. IL4 Receptor-Targeted Proapoptotic Peptide Blocks Tumor Growth and Metastasis by Enhancing Antitumor Immunity. Mol Cancer Ther. 2017;16:2803-2816 pubmed publisher
Sin Y, Price P, Ballantyne L, Funk C. Proof-of-Concept Gene Editing for the Murine Model of Inducible Arginase-1 Deficiency. Sci Rep. 2017;7:2585 pubmed publisher
Jee B, Sharma P, Katoch K, Joshi B, Awasthi S. IL-10 down-regulates the expression of survival associated gene hspX of Mycobacterium tuberculosis in murine macrophage. Braz J Infect Dis. 2017;21:386-390 pubmed publisher
Chang C, Wan J, Li Q, Renfroe S, Heller N, Wang J. Alternative activation-skewed microglia/macrophages promote hematoma resolution in experimental intracerebral hemorrhage. Neurobiol Dis. 2017;103:54-69 pubmed publisher
Dawicki W, Li C, Town J, Zhang X, Gordon J. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3+CD49b-Foxp3- regulatory T cells. J Allergy Clin Immunol. 2017;139:1608-1620.e3 pubmed publisher
Gao S, Li C, Zhu Y, Wang Y, Sui A, Zhong Y, et al. PEDF mediates pathological neovascularization by regulating macrophage recruitment and polarization in the mouse model of oxygen-induced retinopathy. Sci Rep. 2017;7:42846 pubmed publisher
Daria A, Colombo A, Llovera G, Hampel H, Willem M, Liesz A, et al. Young microglia restore amyloid plaque clearance of aged microglia. EMBO J. 2017;36:583-603 pubmed publisher
Schmidt A, Zhang X, Joshi R, Iqbal S, Wahlund C, Gabrielsson S, et al. Human macrophages induce CD4(+)Foxp3(+) regulatory T cells via binding and re-release of TGF-?. Immunol Cell Biol. 2016;94:747-62 pubmed publisher
Zhu Y, Brown J, Sag D, Zhang L, Suttles J. Adenosine 5'-monophosphate-activated protein kinase regulates IL-10-mediated anti-inflammatory signaling pathways in macrophages. J Immunol. 2015;194:584-94 pubmed publisher
Wang J, Cao Z, Zhang X, Nakamura M, Sun M, Hartman J, et al. Novel mechanism of macrophage-mediated metastasis revealed in a zebrafish model of tumor development. Cancer Res. 2015;75:306-15 pubmed publisher
Kuwahara G, Nishinakamura H, Kojima D, Tashiro T, Kodama S. GM-CSF treated F4/80+ BMCs improve murine hind limb ischemia similar to M-CSF differentiated macrophages. PLoS ONE. 2014;9:e106987 pubmed publisher
Hsu K, Chung Y, Endoh Y, Geczy C. TLR9 ligands induce S100A8 in macrophages via a STAT3-dependent pathway which requires IL-10 and PGE2. PLoS ONE. 2014;9:e103629 pubmed publisher
Speck S, Lim J, Shelake S, Matka M, Stoddard J, Farr A, et al. TGF-? signaling initiated in dendritic cells instructs suppressive effects on Th17 differentiation at the site of neuroinflammation. PLoS ONE. 2014;9:e102390 pubmed publisher
Jetten N, Roumans N, Gijbels M, Romano A, Post M, de Winther M, et al. Wound administration of M2-polarized macrophages does not improve murine cutaneous healing responses. PLoS ONE. 2014;9:e102994 pubmed publisher
Li H, Hu D, Fan H, Zhang Y, Lesage G, Caudle Y, et al. ?-Arrestin 2 negatively regulates Toll-like receptor 4 (TLR4)-triggered inflammatory signaling via targeting p38 MAPK and interleukin 10. J Biol Chem. 2014;289:23075-85 pubmed publisher
Zhu E, Wang X, Zheng B, Wang Q, Hao J, Chen S, et al. miR-20b suppresses Th17 differentiation and the pathogenesis of experimental autoimmune encephalomyelitis by targeting ROR?t and STAT3. J Immunol. 2014;192:5599-609 pubmed publisher
Shibata T, Habiel D, Coelho A, Kunkel S, Lukacs N, Hogaboam C. Axl receptor blockade ameliorates pulmonary pathology resulting from primary viral infection and viral exacerbation of asthma. J Immunol. 2014;192:3569-81 pubmed publisher
Veremeyko T, Siddiqui S, Sotnikov I, Yung A, Ponomarev E. IL-4/IL-13-dependent and independent expression of miR-124 and its contribution to M2 phenotype of monocytic cells in normal conditions and during allergic inflammation. PLoS ONE. 2013;8:e81774 pubmed publisher
Kanari Y, Sato Y, Aoyama S, Muta T. Thioredoxin-interacting protein gene expression via MondoA is rapidly and transiently suppressed during inflammatory responses. PLoS ONE. 2013;8:e59026 pubmed publisher
Freeman B, Hammarlund E, Raué H, Slifka M. Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A. 2012;109:9971-6 pubmed publisher
Li B, Zhong L, Yang X, Andersson T, Huang M, Tang S. WNT5A signaling contributes to A?-induced neuroinflammation and neurotoxicity. PLoS ONE. 2011;6:e22920 pubmed publisher
Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, et al. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J Virol. 2010;84:12703-12 pubmed publisher
Findlay E, Greig R, Stumhofer J, Hafalla J, de Souza J, Saris C, et al. Essential role for IL-27 receptor signaling in prevention of Th1-mediated immunopathology during malaria infection. J Immunol. 2010;185:2482-92 pubmed publisher
Ilarregui J, Croci D, Bianco G, Toscano M, Salatino M, Vermeulen M, et al. Tolerogenic signals delivered by dendritic cells to T cells through a galectin-1-driven immunoregulatory circuit involving interleukin 27 and interleukin 10. Nat Immunol. 2009;10:981-91 pubmed publisher
Stoffels B, Schmidt J, Nakao A, Nazir A, Chanthaphavong R, Bauer A. Role of interleukin 10 in murine postoperative ileus. Gut. 2009;58:648-60 pubmed publisher
Tewari N, Kalkunte S, Murray D, Sharma S. The water channel aquaporin 1 is a novel molecular target of polychlorinated biphenyls for in utero anomalies. J Biol Chem. 2009;284:15224-32 pubmed publisher
Wieland C, van der Windt G, Florquin S, McKenzie A, van der Poll T. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect. 2009;11:524-30 pubmed publisher
Wieland C, van der Windt G, Wiersinga W, Florquin S, van der Poll T. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology. 2008;125:272-9 pubmed publisher
Flierl M, Rittirsch D, Gao H, Hoesel L, Nadeau B, Day D, et al. Adverse functions of IL-17A in experimental sepsis. FASEB J. 2008;22:2198-205 pubmed publisher
Franki S, Steward K, Betting D, Kafi K, Yamada R, Timmerman J. Dendritic cells loaded with apoptotic antibody-coated tumor cells provide protective immunity against B-cell lymphoma in vivo. Blood. 2008;111:1504-11 pubmed
Zheng S, Wang J, Wang P, Gray J, Horwitz D. IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells. J Immunol. 2007;178:2018-27 pubmed
Hajjar A, Harvey M, Shaffer S, Goodlett D, Sjostedt A, Edebro H, et al. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Infect Immun. 2006;74:6730-8 pubmed
Osuchowski M, Welch K, Siddiqui J, Remick D. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J Immunol. 2006;177:1967-74 pubmed
Nakagome K, Dohi M, Okunishi K, Tanaka R, Miyazaki J, Yamamoto K. In vivo IL-10 gene delivery attenuates bleomycin induced pulmonary fibrosis by inhibiting the production and activation of TGF-beta in the lung. Thorax. 2006;61:886-94 pubmed
Wen H, Hogaboam C, Gauldie J, Kunkel S. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am J Pathol. 2006;168:1940-50 pubmed
Osuchowski M, Siddiqui J, Copeland S, Remick D. Sequential ELISA to profile multiple cytokines from small volumes. J Immunol Methods. 2005;302:172-81 pubmed
Ellaban E, Bolgos G, Remick D. Selective macrophage suppression during sepsis. Cell Immunol. 2004;231:103-11 pubmed
Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R. IL-22 increases the innate immunity of tissues. Immunity. 2004;21:241-54 pubmed
Sharif M, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv L. IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis. J Immunol. 2004;172:6476-81 pubmed
Chen W, Jin W, Hardegen N, Lei K, Li L, Marinos N, et al. Conversion of peripheral CD4+CD25- naive T cells to CD4+CD25+ regulatory T cells by TGF-beta induction of transcription factor Foxp3. J Exp Med. 2003;198:1875-86 pubmed
image
image 1 :
R&D Systems 417-ML-005 image 1
Recombinant Mouse IL-10 Protein Bioactivity
product information
brand :
R&D Systems, a Bio-Techne brand
catalog number base :
417-ML
SKU :
417-ML-005
product name :
Recombinant Mouse IL-10 Protein
description :
The Recombinant Mouse IL-10 Protein from R&D Systems is derived from E. coli. The Recombinant Mouse IL-10 Protein has been validated for the following applications: Bioactivity.
target :
IL-10
unit size :
5 ug (also 25 ug, 100 ug, 250 ug)
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Mouse
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
observed molecular weight :
17 kDa, reducing conditions
theoretical molecular weight :
18 kDa
gene symbol :
Il10
details of functionality :
Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L.  et al . (1991) J. Exp. Med. 173 :507. The ED50 for this effect is 0.1-0.6 ng/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
NP_034678
applications :
Bioactivity
product keywords :
417-ML, rmIL-10, mIL-10,, 417ML, cytokinegrowthfact, IL10, IL 10, Interleukin 10, Interleukin-10, cytokine, cytokines, IL10A, GVHDS, TGIF, CSIF, Cytokine Synthesis Inhibitory Factor, T-Cell Growth Inhibitory Factor
source :
E. coli-derived Recombinant Mouse IL-10 Protein
USD :
283
USD 2025 :
286 USD
alt names :
CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL10, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.