protein

Recombinant Bovine Enteropeptidase/Enterokinase Protein, CF

4139-SE-010

10 ug

432 USD

4139-SE-010

Recombinant Bovine Enteropeptidase/Enterokinase Protein, CF

10 ug

The Recombinant Bovine Enteropeptidase/Enterokinase Protein, CF from R&D Systems is derived from E. coli. The Recombinant Bovine Enteropeptidase/Enterokinase Protein, CF has been validated for the following applications: Enzyme Activity.

Enteropeptidase/Enterokinase

Proteins and Enzymes

Supplied as a 0.2 ╡m filtered solution in Glycerol, NaCl and HEPES.

Unconjugated

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Bovine

34 kDa, reducing conditions 30 kDa, non-reducing conditions

1.5 kDa (heavy chain C-terminal fragment), 26 kDa (light chain)

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5ÆDithio-bis (2-nitrobenzoic acid) (DTNB). Lu, D. et al . (1997) J. Biol. Chem. 272 :31293. The specific activity is 35 nmol/min/╡g, as measured under the described conditions.

<1.0 EU per 1 ╡g of the protein by the LAL method.

P98072

Enzyme Activity

E. coli -derived bovine Enteropeptidase/Enterokinase protein Cys788-Lys800 (heavy chain C-terminal fragment) with an N-terminal Ala, & Ile801-His1035 (light chain)

E. coli

432 USD

EC 3.4.21, EC 3.4.21.9, Enterokinase, ENTK, ENTKenterokinase, MGC133046, protease, serine, 7 (enterokinase), PRSS7, PRSS7enteropeptidase, Serine protease 7, TMPRSS15, Transmembrane protease serine 15, transmembrane protease, serine 15

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 ░C as supplied. 3 months, -20 to -70 ░C under sterile conditions after opening.