Recombinant Mouse R-Spondin 3 Protein | R&D Systems 4120-RS-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse R-Spondin 3 Protein

catalog :

4120-RS-025

quantity :

25 ug

price :

456 USD

more info or order :

R&D Systems product webpage

citations: 13

Reference
Nagano K, Yamana K, Saito H, Kiviranta R, Pedroni A, Raval D, et al. R-spondin 3 deletion induces Erk phosphorylation to enhance Wnt signaling and promote bone formation in the appendicular skeleton. elife. 2022;11: pubmed publisher
Mita Y, Zhu H, Furuichi Y, Hamaguchi H, Manabe Y, Fujii N. R-spondin3 is a myokine that differentiates myoblasts to type I fibres. Sci Rep. 2022;12:13020 pubmed publisher
Nilsson K, Henning P, El Shahawy M, Nethander M, Andersen T, Ejersted C, et al. RSPO3 is important for trabecular bone and fracture risk in mice and humans. Nat Commun. 2021;12:4923 pubmed publisher
Zhang M, Haughey M, Wang N, Blease K, Kapoun A, Couto S, et al. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs. PLoS ONE. 2020;15:e0229445 pubmed publisher
Dickinson K, Hammond L, Karner C, Hastie N, Carroll T, Goodyer P. Molecular determinants of WNT9b responsiveness in nephron progenitor cells. PLoS ONE. 2019;14:e0215139 pubmed publisher
Ogasawara R, Hashimoto D, Kimura S, Hayase E, Ara T, Takahashi S, et al. Intestinal Lymphatic Endothelial Cells Produce R-Spondin3. Sci Rep. 2018;8:10719 pubmed publisher
Greicius G, Kabiri Z, Sigmundsson K, Liang C, Bunte R, Singh M, et al. PDGFR?+ pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo. Proc Natl Acad Sci U S A. 2018;115:E3173-E3181 pubmed publisher
Liu S, Yin Y, Yu R, Li Y, Zhang W. R-spondin3-LGR4 signaling protects hepatocytes against DMOG-induced hypoxia/reoxygenation injury through activating Î²-catenin. Biochem Biophys Res Commun. 2018;499:59-65 pubmed publisher
Yu Y, Moberly A, Bhattarai J, Duan C, Zheng Q, Li F, et al. The Stem Cell Marker Lgr5 Defines a Subset of Postmitotic Neurons in the Olfactory Bulb. J Neurosci. 2017;37:9403-9414 pubmed publisher
Yin X, Yi H, Wang L, Wu W, Wu X, Yu L. RSPOs facilitated HSC activation and promoted hepatic fibrogenesis. Oncotarget. 2016;7:63767-63778 pubmed publisher
Cambier L, Plate M, Sucov H, Pashmforoush M. Nkx2-5 regulates cardiac growth through modulation of Wnt signaling by R-spondin3. Development. 2014;141:2959-71 pubmed publisher
Li J, Chai B, Zhang W, Fritze D, Zhang C, Mulholland M. LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats. Endocrinology. 2014;155:429-40 pubmed publisher
Luo W, Rodriguez M, Valdez J, Zhu X, Tan K, Li D, et al. Lgr4 is a key regulator of prostate development and prostate stem cell differentiation. Stem Cells. 2013;31:2492-505 pubmed publisher

product information

master code :

4120-RS

SKU :

4120-RS-025

product name :

Recombinant Mouse R-Spondin 3 Protein

unit size :

25 ug

description :

The Recombinant Mouse R-Spondin 3 Protein from R&D Systems is derived from CHO. The Recombinant Mouse R-Spondin 3 Protein has been validated for the following applications: Bioactivity.

target :

R-Spondin 3

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Mouse

observed molecular weight :

37-43 kDa, reducing conditions

theoretical molecular weight :

28.9 kDa

gene symbol :

Rspo3

details of functionality :

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.25-1 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q2TJ95

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 3 protein Met1-His277

source short :

CHO

USD :

456 USD

alt names :

Cristin 1, CRISTIN1, FLJ14440, hPWTSR, hRspo3, Protein with TSP type-1 repeat, PWTSR, Roof plate-specific spondin-3, RSPO3, RSpondin 3, R-spondin 3 homolog (Xenopus laevis), R-spondin-3, Thrombospondin type-1 domain-containing protein 2, thrombospondin, type I, domain containing 2, THSD2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage