product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse TNF-alpha (aa 84-235) Protein
catalog :
410-TRNC-010
quantity :
10 ug (also 50 ug)
price :
305 USD
more info or order :
R&D Systems product webpage
citations: 5
Reference
Ji D, Yamashiro L, Chen K, Mukaida N, Kramnik I, Darwin K, et al. Type I interferon-driven susceptibility to Mycobacterium tuberculosis is mediated by IL-1Ra. Nat Microbiol. 2019;4:2128-2135 pubmed publisher
Garner K, Betin V, Pinto V, Graham M, Abgueguen E, Barnes M, et al. Enhanced insulin receptor, but not PI3K, signalling protects podocytes from ER stress. Sci Rep. 2018;8:3902 pubmed publisher
Jules J, Wang S, Shi Z, Liu J, Wei S, Feng X. The IVVY Motif and Tumor Necrosis Factor Receptor-associated Factor (TRAF) Sites in the Cytoplasmic Domain of the Receptor Activator of Nuclear Factor κB (RANK) Cooperate to Induce Osteoclastogenesis. J Biol Chem. 2015;290:23738-50 pubmed publisher
Jules J, Shi Z, Liu J, Xu D, Wang S, Feng X. Receptor activator of NF-{kappa}B (RANK) cytoplasmic IVVY535-538 motif plays an essential role in tumor necrosis factor-{alpha} (TNF)-mediated osteoclastogenesis. J Biol Chem. 2010;285:37427-35 pubmed publisher
Liu W, Xu D, Yang H, Xu H, Shi Z, Cao X, et al. Functional identification of three receptor activator of NF-kappa B cytoplasmic motifs mediating osteoclast differentiation and function. J Biol Chem. 2004;279:54759-69 pubmed
product information
brand :
R&D Systems, a Bio-Techne Brand
catalog number base :
410-TRNC
SKU :
410-TRNC-010
product name :
Recombinant Mouse TNF-alpha (aa 84-235) Protein
Description :
The Recombinant Mouse TNF-alpha (aa 84-235) Protein from R&D Systems is derived from E. coli. The Recombinant Mouse TNF-alpha (aa 84-235) Protein has been validated for the following applications: Bioactivity.
target :
TNF-alpha
category :
Proteins and Enzymes
unit size :
10 ug (also 50 ug)
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Mouse
theoretical molecular weight :
17 kDa (monomer)
gene symbol :
Tnf
details of functionality :
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 0.8-4 pg/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
P06804
applications :
Bioactivity
source :
E. coli-derived Recombinant Mouse TNF-alpha (aa 84-235) Protein
USD :
305 USD
alt names :
APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.