Recombinant Human Fibronectin Fragment 3 Protein, CF | R&D Systems 3938-FN-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Fibronectin Fragment 3 Protein, CF

catalog :

3938-FN-050

quantity :

50 ug

price :

303 USD

more info or order :

R&D Systems product webpage

citations: 2

Reference
Liamas E, Black R, Mulheran P, Tampe R, Wieneke R, Thomas O, et al. Probing fibronectin adsorption on chemically defined surfaces by means of single molecule force microscopy. Sci Rep. 2020;10:15662 pubmed publisher
Oh Y, Hubauer Brenner M, Gruber H, Cui Y, Traxler L, Siligan C, et al. Curli mediate bacterial adhesion to fibronectin via tensile multiple bonds. Sci Rep. 2016;6:33909 pubmed publisher

product information

master code :

3938-FN

SKU :

3938-FN-050

product name :

Recombinant Human Fibronectin Fragment 3 Protein, CF

unit size :

50 ug

description :

The Recombinant Human Fibronectin Fragment 3 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Fibronectin Fragment 3 Protein, CF has been validated for the following applications: Bioactivity.

target :

Fibronectin

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS and Tween« 20.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

80-90 kDa, reducing conditions

theoretical molecular weight :

70.1 kDa

gene symbol :

FN1

details of functionality :

Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. When 5 x 104 cells/well are added to Recombinant Human Fibronectin Fragment 3/amino acids 1266-1908 coated plates (1 ╡g/mL with 100 ╡L/well), approximately 50-75% will adhere after 30-60 minutes at 37 ░C. Optimal concentration depends on cell type as well as the application or research objective.

endotoxin note :

<0.01 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_997647.2

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human Fibronectin protein Glu1266-Pro1908 (Met1874Thr), with a C-terminal 6-His tag

source short :

NS0

USD :

303 USD

alt names :

CIG, ED-B, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF, SMDCF

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage