Recombinant Human ECM1 Protein, CF | R&D Systems 3937-EC-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human ECM1 Protein, CF

catalog :

3937-EC-050

quantity :

50 ug

price :

456 USD

more info or order :

R&D Systems product webpage

citations: 2

Reference
Yin H, Wang J, Li H, Yu Y, Wang X, Lu L, et al. Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness. Nat Commun. 2021;12:4230 pubmed publisher
Al Shareef Z, Kardooni H, Murillo Garzón V, Domenici G, Stylianakis E, Steel J, et al. Protective effect of stromal Dickkopf-3 in prostate cancer: opposing roles for TGFBI and ECM-1. Oncogene. 2018;37:5305-5324 pubmed publisher

product information

master code :

3937-EC

SKU :

3937-EC-050

product name :

Recombinant Human ECM1 Protein, CF

unit size :

50 ug

description :

The Recombinant Human ECM1 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human ECM1 Protein, CF has been validated for the following applications: Bioactivity.

target :

ECM1

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

observed molecular weight :

77-87 kDa, reducing conditions

theoretical molecular weight :

59.7 kDa

gene symbol :

ECM1

details of functionality :

Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. When 5 x 104 cells/well are added to Recombinant Human ECM-1 coated plates (10 ╡g/mL with 100 ╡L/well), 30% will adhere after 30 minutes at 37 ░C. Optimal concentration depends on cell type as well as the application or research objective.

endotoxin note :

<0.01 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

AAH23505

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human ECM1 protein Ala20-Glu540, with a C-terminal 6-His tag

source short :

NS0

USD :

456 USD

alt names :

ECM1, extracellular matrix protein 1, p85, Secretory Component Glycoprotein, Secretory Component P85

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage