This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human CD14 Protein
catalog :
383-CD
quantity :
50 ug
price :
329 USD
citations: 18
Reference
Zheng M, Ambesi A, McKeown Longo P. Role of TLR4 Receptor Complex in the Regulation of the Innate Immune Response by Fibronectin. Cells. 2020;9: pubmed publisher
Higgins S, De Ceunynck K, Kellum J, Chen X, Gu X, Chaudhry S, et al. Tie2 protects the vasculature against thrombus formation in systemic inflammation. J Clin Invest. 2018;128:1471-1484 pubmed publisher
S Lashkari B, Anumba D. Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4. PLoS ONE. 2017;12:e0173646 pubmed publisher
Wensink A, Kok H, Meeldijk J, Fermie J, Froelich C, Hack C, et al. Granzymes A and K differentially potentiate LPS-induced cytokine response. Cell Death Discov. 2016;2:16084 pubmed publisher
He Z, Riva M, Björk P, Swärd K, Morgelin M, Leanderson T, et al. CD14 Is a Co-Receptor for TLR4 in the S100A9-Induced Pro-Inflammatory Response in Monocytes. PLoS ONE. 2016;11:e0156377 pubmed publisher
Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, et al. Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort. PLoS ONE. 2016;11:e0148073 pubmed publisher
Ma C, Chang W, Shi G, Chang B, Cheng S, Shih Y, et al. Recombinant thrombomodulin inhibits lipopolysaccharide-induced inflammatory response by blocking the functions of CD14. J Immunol. 2015;194:1905-15 pubmed publisher
Fang L, Xu Z, Wang G, Ji F, Mei C, Liu J, et al. Directed evolution of an LBP/CD14 inhibitory peptide and its anti-endotoxin activity. PLoS ONE. 2014;9:e101406 pubmed publisher
Gupta N, Li W, Willard B, Silverstein R, McIntyre T. Proteasome proteolysis supports stimulated platelet function and thrombosis. Arterioscler Thromb Vasc Biol. 2014;34:160-8 pubmed publisher
Kim J, Yoon Y, Lee J, Choi E, Yi N, Park K, et al. Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo. PLoS ONE. 2013;8:e59276 pubmed publisher
Feng C, Stamatos N, Dragan A, Medvedev A, Whitford M, Zhang L, et al. Sialyl residues modulate LPS-mediated signaling through the Toll-like receptor 4 complex. PLoS ONE. 2012;7:e32359 pubmed publisher
Legat A, Thomas S, Hermand P, Van Mechelen M, Goldman M, De Wit D. CD14-independent responses induced by a synthetic lipid A mimetic. Eur J Immunol. 2010;40:797-802 pubmed publisher
Chui J, Li M, Di Girolamo N, Chang J, McCluskey P, Wakefield D. Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex. Invest Ophthalmol Vis Sci. 2010;51:2558-67 pubmed publisher
Zunt S, Burton L, Goldblatt L, Dobbins E, Srinivasan M. Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells. Clin Exp Immunol. 2009;156:285-93 pubmed publisher
Gonzalez R, Seurynck Servoss S, Crowley S, Brown M, Omenn G, Hayes D, et al. Development and validation of sandwich ELISA microarrays with minimal assay interference. J Proteome Res. 2008;7:2406-14 pubmed publisher
Patel D, Bailey S, Gresham J, Schuchman D, Shelhamer J, Goldstein B, et al. TLR4-NOX4-AP-1 signaling mediates lipopolysaccharide-induced CXCR6 expression in human aortic smooth muscle cells. Biochem Biophys Res Commun. 2006;347:1113-20 pubmed
Blais D, Harrold J, Altosaar I. Killing the messenger in the nick of time: persistence of breast milk sCD14 in the neonatal gastrointestinal tract. Pediatr Res. 2006;59:371-6 pubmed
Putnins E, Sanaie A, Wu Q, Firth J. Induction of keratinocyte growth factor 1 Expression by lipopolysaccharide is regulated by CD-14 and toll-like receptors 2 and 4. Infect Immun. 2002;70:6541-8 pubmed
product information
brand :
R&D Systems
master code :
383-CD
SKU :
383-CD-050
product name :
Recombinant Human CD14 Protein
description :
The Recombinant Human CD14 Protein from R&D Systems is derived from CHO. The Recombinant Human CD14 Protein has been validated for the following applications: Bioactivity.
target :
CD14
category :
Proteins and Enzymes
unit size :
50 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
observed molecular weight :
43-58 kDa, reducing conditions
theoretical molecular weight :
35.8 kDa
gene symbol :
CD14
details of functionality :
Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 0.75-4.5 ng/mL.
endotoxin note :
<1.0 EU per 1 µg of the protein by the LAL method.
catalog number base :
383-CD
accessionNumbers :
P08571
applications :
Bioactivity
source :
Chinese Hamster Ovary cell line, CHO-derived human CD14 protein Thr20-Cys352
2020 USD :
329
2021 USD :
329 USD
alt names :
CD14, CD14 antigen, CD14 molecule, monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.