Recombinant Human TRAIL/TNFSF10 (E. coli-expressed), CF | R&D Systems 375-TEC-010/CF product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human TRAIL/TNFSF10 (E. coli-expressed), CF

catalog :

375-TEC-010/CF

quantity :

10 ug

price :

394 USD

more info or order :

R&D Systems product webpage

citations: 10

Reference
Frank Bertoncelj M, Pisetsky D, Kolling C, Michel B, Gay R, Jungel A, et al. TLR3 Ligand Poly(I:C) Exerts Distinct Actions in Synovial Fibroblasts When Delivered by Extracellular Vesicles. Front Immunol. 2018;9:28 pubmed publisher
Zoller V, Funcke J, Roos J, Dahlhaus M, Abd El Hay M, Holzmann K, et al. Trail (TNF-related apoptosis-inducing ligand) induces an inflammatory response in human adipocytes. Sci Rep. 2017;7:5691 pubmed publisher
Chang L, Li Y, Kaplan D. Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis. Sci Rep. 2016;6:36862 pubmed publisher
Roybal K, Williams J, Morsut L, Rupp L, Kolinko I, Choe J, et al. Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Cell. 2016;167:419-432.e16 pubmed publisher
Bozza W, Di X, Takeda K, Rivera Rosado L, Pariser S, Zhang B. The use of a stably expressed FRET biosensor for determining the potency of cancer drugs. PLoS ONE. 2014;9:e107010 pubmed publisher
Guicciardi M, Werneburg N, Bronk S, Franke A, Yagita H, Thomas G, et al. Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity. PLoS ONE. 2014;9:e92124 pubmed publisher
Park S, Kim M, Kim H, Kang C, Kim S. Sensitization of imatinib-resistant CML cells to TRAIL-induced apoptosis is mediated through down-regulation of Bcr-Abl as well as c-FLIP. Biochem J. 2009;420:73-81 pubmed publisher
Roux S, Lambert Comeau P, Saint Pierre C, Lépine M, Sawan B, Parent J. Death receptors, Fas and TRAIL receptors, are involved in human osteoclast apoptosis. Biochem Biophys Res Commun. 2005;333:42-50 pubmed
Chopin V, Slomianny C, Hondermarck H, Le Bourhis X. Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1). Exp Cell Res. 2004;298:560-73 pubmed
Sade H, Krishna S, Sarin A. The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells. J Biol Chem. 2004;279:2937-44 pubmed

product information

brand :

R&D Systems

master code :

375-TEC/CF

SKU :

375-TEC-010/CF

product name :

Recombinant Human TRAIL/TNFSF10 (E. coli-expressed), CF

description :

The Recombinant Human TRAIL/TNFSF10 (E. coli-expressed), CF from R&D Systems is derived from E. coli. The Recombinant Human TRAIL/TNFSF10 (E. coli-expressed), CF has been validated for the following applications: Bioactivity.

target :

TRAIL/TNFSF10

category :

Proteins and Enzymes

sizes available :

10 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in Tris, (NH4)2SO4 and ZnSO4.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

theoretical molecular weight :

19 kDa (monomer)

gene symbol :

TNFSF10

details of functionality :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 0.5-6 ng/mL. Note: This is one of multiple forms available for this protein. Check R D Systems' website, www.RnDSystems.com , for a complete listing of the variants.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

Q6IBA9

applications :

Bioactivity

source :

E. coli-derived Recombinant Human TRAIL/TNFSF10 (E. coli-expressed), CF

USD :

388

USD 2023 :

394 USD

alt names :

Apo-2 ligand, APO2L, Apo-2Ltumor necrosis factor (ligand) family, member 10, APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta, CD253, CD253 antigen, Protein TRAIL, TL2, TNF-related apoptosis-inducing ligand, TNFSF10, TRAILTNF-related apoptosis inducing ligand TRAIL, tumor necrosis factor (ligand) superfamily, member 10, tumor necrosis factor ligand superfamily member 10

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 2 weeks, 2 to 8 °C under sterile conditions after reconstitution. 1 month, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage