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product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Ephrin-A5 Fc Chimera Protein, CF
catalog :
374-EA-200
quantity :
200 ug
price :
359 USD
citations: 37
Reference
Madasu C, Liao Z, Parks S, Sharma K, Bohren K, Ye Q, et al. Identification of potent pan-ephrin receptor kinase inhibitors using DNA-encoded chemistry technology. Proc Natl Acad Sci U S A. 2024;121:e2322934121 pubmed publisher
Banerjee S, Lessard F, Chartier F, Jacquet K, Osornio Hernandez A, Teyssier V, et al. EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks. Cell Rep. 2022;40:111031 pubmed publisher
Cai C, Zhang M, Liu L, Zhang H, Guo Y, Lan T, et al. ADAM10-cleaved ephrin-A5 contributes to prostate cancer metastasis. Cell Death Dis. 2022;13:453 pubmed publisher
Creighton B, Afriyie S, Ajit D, Casingal C, Voos K, Reger J, et al. Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A. elife. 2021;10: pubmed publisher
Teo L, Boghdadi A, Homman Ludiye J, Mundiñano I, Kwan W, Bourne J. Replicating infant-specific reactive astrocyte functions in the injured adult brain. Prog Neurobiol. 2021;204:102108 pubmed publisher
Light T, Gómez Soler M, Wang Z, Karl K, Zapata Mercado E, Gehring M, et al. A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain. J Biol Chem. 2021;297:100876 pubmed publisher
Iguchi T, Oka Y, Yasumura M, Omi M, Kuroda K, Yagi H, et al. Mutually Repulsive EphA7-EfnA5 Organize Region-to-Region Corticopontine Projection by Inhibiting Collateral Extension. J Neurosci. 2021;41:4795-4808 pubmed publisher
Jukonen J, Moyano Galceran L, Höpfner K, Pietilä E, Lehtinen L, Huhtinen K, et al. Aggressive and recurrent ovarian cancers upregulate ephrinA5, a non-canonical effector of EphA2 signaling duality. Sci Rep. 2021;11:8856 pubmed publisher
Chen Y, Yen Y, Jang C, Wang S, Huang H, Chen C, et al. Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells. Sci Rep. 2021;11:644 pubmed publisher
Verheyen T, Fang T, Lindenhofer D, Wang Y, Akopyan K, Lindqvist A, et al. Spatial organization-dependent EphA2 transcriptional responses revealed by ligand nanocalipers. Nucleic Acids Res. 2020;48:5777-5787 pubmed publisher
Laing E, Navaratnarajah C, Cheliout Da Silva S, Petzing S, Xu Y, Sterling S, et al. Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus. Proc Natl Acad Sci U S A. 2019;116:20707-20715 pubmed publisher
Habiel D, Espíndola M, Jones I, Coelho A, STRIPP B, Hogaboam C. CCR10+ epithelial cells from idiopathic pulmonary fibrosis lungs drive remodeling. JCI Insight. 2018;3: pubmed publisher
Teng T, Gaillard A, Muzerelle A, Gaspar P. EphrinA5 Signaling Is Required for the Distinctive Targeting of Raphe Serotonin Neurons in the Forebrain. Eneuro. 2017;4: pubmed publisher
Sullivan C, Kümper M, Temple B, Maness P. The Neural Cell Adhesion Molecule (NCAM) Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce Ras Homolog Gene Family, Member A (RhoA)/Rho-associated protein kinase (ROCK)-mediated Growth Cone Collapse. J Biol Chem. 2016;291:26262-26272 pubmed
Lee H, Noh H, Mun J, Gu C, Sever S, Park S. Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis. Nat Commun. 2016;7:12799 pubmed publisher
Martinez Martin N, Ramani S, Hackney J, Tom I, Wranik B, Chan M, et al. The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation. Nat Commun. 2016;7:11473 pubmed publisher
Gerstmann K, Pensold D, Symmank J, Khundadze M, Hübner C, Bolz J, et al. Thalamic afferents influence cortical progenitors via ephrin A5-EphA4 interactions. Development. 2015;142:140-50 pubmed publisher
Maury Y, Côme J, Piskorowski R, Salah Mohellibi N, Chevaleyre V, Peschanski M, et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol. 2015;33:89-96 pubmed publisher
Schaupp A, Sabet O, Dudanova I, Ponserre M, Bastiaens P, Klein R. The composition of EphB2 clusters determines the strength in the cellular repulsion response. J Cell Biol. 2014;204:409-22 pubmed publisher
Szepietowska B, Horvath T, Sherwin R. Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia. Diabetes. 2014;63:1140-7 pubmed publisher
Defourny J, Poirrier A, Lallemend F, Mateo Sánchez S, Neef J, Vanderhaeghen P, et al. Ephrin-A5/EphA4 signalling controls specific afferent targeting to cochlear hair cells. Nat Commun. 2013;4:1438 pubmed publisher
Tai A, Cassidy R, Kromer L. EphA7 expression identifies a unique neuronal compartment in the rat striatum. J Comp Neurol. 2013;521:2663-79 pubmed publisher
Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin R. EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia. Diabetes. 2013;62:1282-8 pubmed publisher
Park E, Kim Y, Noh H, Lee H, Yoo S, Park S. EphA/ephrin-A signaling is critically involved in region-specific apoptosis during early brain development. Cell Death Differ. 2013;20:169-80 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Genander M, Holmberg J, Frisen J. Ephrins negatively regulate cell proliferation in the epidermis and hair follicle. Stem Cells. 2010;28:1196-205 pubmed publisher
Afshari F, Kwok J, Fawcett J. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J Neurosci. 2010;30:4246-55 pubmed publisher
Torii M, Hashimoto Torii K, Levitt P, Rakic P. Integration of neuronal clones in the radial cortical columns by EphA and ephrin-A signalling. Nature. 2009;461:524-8 pubmed publisher
Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisen J, et al. Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction. PLoS ONE. 2008;3:e3322 pubmed publisher
Wykosky J, Palma E, Gibo D, Ringler S, Turner C, Debinski W. Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor. Oncogene. 2008;27:7260-73 pubmed publisher
Lim Y, McLaughlin T, Sung T, Santiago A, Lee K, O Leary D. p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping. Neuron. 2008;59:746-58 pubmed publisher
Fitzgerald M, Buckley A, Lukehurst S, Dunlop S, Beazley L, Rodger J. Neurite responses to ephrin-A5 modulated by BDNF: evidence for TrkB-EphA interactions. Biochem Biophys Res Commun. 2008;374:625-30 pubmed publisher
Zimmer G, Garcez P, Rudolph J, Niehage R, Weth F, Lent R, et al. Ephrin-A5 acts as a repulsive cue for migrating cortical interneurons. Eur J Neurosci. 2008;28:62-73 pubmed publisher
Konstantinova I, Nikolova G, Ohara Imaizumi M, Meda P, Kucera T, Zarbalis K, et al. EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets. Cell. 2007;129:359-70 pubmed
Janes P, Saha N, Barton W, Kolev M, Wimmer Kleikamp S, Nievergall E, et al. Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans. Cell. 2005;123:291-304 pubmed
Muñoz L, Zayachkivsky A, Kunz R, Hunt J, Wang G, Scott S. Ephrin-A5 inhibits growth of embryonic sensory neurons. Dev Biol. 2005;283:397-408 pubmed
Sharfe N, Freywald A, Toro A, Dadi H, Roifman C. Ephrin stimulation modulates T cell chemotaxis. Eur J Immunol. 2002;32:3745-55 pubmed
product information
master code :
374-EA
SKU :
374-EA-200
product name :
Recombinant Human Ephrin-A5 Fc Chimera Protein, CF
unit size :
200 ug
description :
The Recombinant Human Ephrin-A5 Fc Chimera Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Ephrin-A5 Fc Chimera Protein, CF has been validated for the following applications: Binding Activity.
target :
Ephrin-A5
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 ╡m filtered solution in PBS.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
species :
Human
observed molecular weight :
50-55 kDa, reducing conditions
theoretical molecular weight :
48.6 kDa (monomer)
gene symbol :
EFNA5
details of functionality :
Measured by its ability to compete with Biotinylated Recombinant Human Ephrin-A5 Fc Chimera (Catalog # BT374 ) for binding to immobilized recombinant mouse Eph-A3 Fc Chimera in a functional ELISA assay. Optimal dilutions should be determined by each laboratory for each application.
endotoxin note :
<0.10 EU per 1 ╡g of the protein by the LAL method.
accessionNumbers :
P52803
applications :
Binding Activity
source long :
Mouse myeloma cell line, NS0-derived human Ephrin-A5 protein Human Ephrin-A5 (Gln21-Asn203) Accession # P52803 IEGRMD Human IgG1 (Pro100-Lys330) 6-His tag N-terminus C-terminus
source short :
NS0
USD :
359 USD
alt names :
AF1, AL-1, EFL-5, EFNA5, EPH-related receptor tyrosine kinase ligand 7, ephrin-A5, EphrinA5, EPLG7, GLC1M, LERK-7, LERK7EFL5, RAGS
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.