Recombinant Mouse Wnt-9b Protein | R&D Systems 3669-WN-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse Wnt-9b Protein

catalog :

3669-WN-025

quantity :

25 ug

price :

554 USD

citations: 9

Reference
Gupta S, Kawaguchi R, Heinrichs E, Gallardo S, Castellanos S, Mandric I, et al. In vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion. Cell Rep. 2022;40:111119 pubmed publisher
Chang C, Liang M, Wu C, Huang P, Chen H, Yet S, et al. WNT3A Promotes Neuronal Regeneration upon Traumatic Brain Injury. Int J Mol Sci. 2020;21: pubmed publisher
Dickinson K, Hammond L, Karner C, Hastie N, Carroll T, Goodyer P. Molecular determinants of WNT9b responsiveness in nephron progenitor cells. PLoS ONE. 2019;14:e0215139 pubmed publisher
Kilander M, Dahlström J, Schulte G. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal. 2014;26:1943-9 pubmed publisher
Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, et al. Noncanonical Wnt5a enhances Wnt/?-catenin signaling during osteoblastogenesis. Sci Rep. 2014;4:4493 pubmed publisher
Thrasivoulou C, Millar M, Ahmed A. Activation of intracellular calcium by multiple Wnt ligands and translocation of ?-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/?-catenin pathways. J Biol Chem. 2013;288:35651-9 pubmed publisher
Goel S, Chin E, Fakhraldeen S, Berry S, Beebe D, Alexander C. Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts. J Biol Chem. 2012;287:16454-66 pubmed publisher
Gong Y, Bourhis E, Chiu C, Stawicki S, DeAlmeida V, Liu B, et al. Wnt isoform-specific interactions with coreceptor specify inhibition or potentiation of signaling by LRP6 antibodies. PLoS ONE. 2010;5:e12682 pubmed publisher
Bourhis E, Tam C, Franke Y, Bazan J, Ernst J, Hwang J, et al. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J Biol Chem. 2010;285:9172-9 pubmed publisher

product information

master code :

3669-WN

SKU :

3669-WN-025

product name :

Recombinant Mouse Wnt-9b Protein

unit size :

25 ug

description :

The Recombinant Mouse Wnt-9b Protein from R&D Systems is derived from CHO. The Recombinant Mouse Wnt-9b Protein has been validated for the following applications: Bioactivity.

target :

Wnt-9b

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Mouse

observed molecular weight :

39-41 kDa, reducing conditions

theoretical molecular weight :

36.8 kDa

gene symbol :

Wnt9b

details of functionality :

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The typical ED50 range is 100-400 ng/mL.

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

O35468

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-9b protein Ala24-Arg359

source short :

CHO

USD :

554 USD

alt names :

Protein Wnt-14b, wingless-type MMTV integration site family, member 15, wingless-type MMTV integration site family, member 9B, Wnt-14b, WNT14Bprotein Wnt-9b, Wnt-15, WNT15Protein Wnt-15, Wnt9b

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution.