Recombinant Human CX3CL1/Fractalkine (Full Length) Protein | R&D Systems 365-FR product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human CX3CL1/Fractalkine (Full Length) Protein

catalog :

365-FR

quantity :

25 ug

price :

329 USD

citations: 18

Reference
Saito K, Iioka H, Maruyama S, Sumardika I, Sakaguchi M, Kondo E. PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment. Neoplasia. 2019;21:1121-1132 pubmed publisher
Collar A, Swamydas M, O Hayre M, Sajib M, Hoffman K, Singh S, et al. The homozygous CX3CR1-M280 mutation impairs human monocyte survival. JCI Insight. 2018;3: pubmed publisher
Bolos M, Llorens Martín M, Perea J, Jurado Arjona J, Rabano A, Hernandez F, et al. Absence of CX3CR1 impairs the internalization of Tau by microglia. Mol Neurodegener. 2017;12:59 pubmed publisher
Siddiqui I, Erreni M, van Brakel M, Debets R, Allavena P. Enhanced recruitment of genetically modified CX3CR1-positive human T cells into Fractalkine/CX3CL1 expressing tumors: importance of the chemokine gradient. J Immunother Cancer. 2016;4:21 pubmed publisher
Cederblad L, Rosengren B, Ryberg E, Hermansson N. AZD8797 is an allosteric non-competitive modulator of the human CX3CR1 receptor. Biochem J. 2016;473:641-9 pubmed publisher
Ayala Lujan J, Vijayakumar V, Gong M, Smith R, Santiago A, Ruiz Perez F. Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes. PLoS ONE. 2014;9:e107920 pubmed publisher
van Gils J, Ramkhelawon B, Fernandes L, Stewart M, Guo L, Seibert T, et al. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol. 2013;33:911-9 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Truman L, Ford C, Pasikowska M, Pound J, Wilkinson S, Dumitriu I, et al. CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis. Blood. 2008;112:5026-36 pubmed publisher
Hermand P, Pincet F, Carvalho S, Ansanay H, Trinquet E, Daoudi M, et al. Functional adhesiveness of the CX3CL1 chemokine requires its aggregation. Role of the transmembrane domain. J Biol Chem. 2008;283:30225-34 pubmed publisher
Zanchi C, Zoja C, Morigi M, Valsecchi F, Liu X, Rottoli D, et al. Fractalkine and CX3CR1 mediate leukocyte capture by endothelium in response to Shiga toxin. J Immunol. 2008;181:1460-9 pubmed
Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T. Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration. J Leukoc Biol. 2008;84:215-23 pubmed publisher
You J, Yang C, Huang J, Chen M, Yang C. Fractalkine, a CX3C chemokine, as a mediator of ocular angiogenesis. Invest Ophthalmol Vis Sci. 2007;48:5290-8 pubmed
Hundhausen C, Schulte A, Schulz B, Andrzejewski M, Schwarz N, von Hundelshausen P, et al. Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes. J Immunol. 2007;178:8064-72 pubmed
Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, et al. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J Immunol. 2004;173:6592-602 pubmed
Bolovan Fritts C, Trout R, Spector S. Human cytomegalovirus-specific CD4+-T-cell cytokine response induces fractalkine in endothelial cells. J Virol. 2004;78:13173-81 pubmed
Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao J, Maho M, et al. Fractalkine mediates natural killer-dependent antitumor responses in vivo. Cancer Res. 2003;63:7468-74 pubmed
Zhang Q, Shimoya K, Ohta Y, Chin R, Tenma K, Isaka S, et al. Detection of fractalkine in human seminal plasma and its role in infertile patients. Hum Reprod. 2002;17:1560-4 pubmed

product information

brand :

R&D Systems

master code :

365-FR

SKU :

365-FR-025

product name :

Recombinant Human CX3CL1/Fractalkine (Full Length) Protein

description :

The Recombinant Human CX3CL1/Fractalkine (Full Length) Protein from R&D Systems is derived from NS0. The Recombinant Human CX3CL1/Fractalkine (Full Length) Protein has been validated for the following applications: Bioactivity.

target :

CX3CL1/Fractalkine

category :

Proteins and Enzymes

unit size :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Human

observed molecular weight :

90 kDa, reducing conditions

theoretical molecular weight :

35 kDa

gene symbol :

CX3CL1

details of functionality :

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED50 for this effect is 20-100 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CX3CR1. The ED50 for this effect is 2.5-10 ng/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

catalog number base :

365-FR

accessionNumbers :

P78423

applications :

Bioactivity

source :

Mouse myeloma cell line, NS0-derived human CX3CL1/Fractalkine protein Human CX3CL 1 (Gln25 - Arg339) (Ser199Asn) Accession # P78423 KQN 6-His tag N-terminus C-terminus

2020 USD :

329

2021 USD :

329 USD

alt names :

ABCD-3, C3Xkine, chemokine (C-X3-C motif) ligand 1, CX3C membrane-anchored chemokine, CXC3, CXC3C, FKN, fractalkine, Neurotactin, neurotactin), NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, NTTSmall-inducible cytokine D1, SCYD1C-X3-C motif chemokine 1, small inducible cytokine subfamily D (Cys-X3-Cys), member-1

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.