Recombinant Mouse ASAH2 Protein, CF | R&D Systems 3558-AH-010 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Mouse ASAH2 Protein, CF

catalog :

3558-AH-010

quantity :

10 ug

price :

477 USD

more info or order :

R&D Systems product webpage

citations: 3

Reference
Sun R, Gu X, Lei C, Chen L, Chu S, Xu G, et al. Neutral ceramidase-dependent regulation of macrophage metabolism directs intestinal immune homeostasis and controls enteric infection. Cell Rep. 2022;38:110560 pubmed publisher
Rice T, Seitz A, Edwards M, Gulbins E, Caldwell C. Frontline Science: Sphingosine rescues burn-injured mice from pulmonary Pseudomonas aeruginosa infection. J Leukoc Biol. 2016;100:1233-1237 pubmed
Chen B, Chang H, Hsu M, Shih C, Chiu Y, Chiu W, et al. Peptidoglycan induces cyclooxygenase-2 expression in macrophages by activating the neutral sphingomyelinase-ceramide pathway. J Biol Chem. 2009;284:20562-73 pubmed publisher

product information

master code :

3558-AH

SKU :

3558-AH-010

product name :

Recombinant Mouse ASAH2 Protein, CF

unit size :

10 ug

description :

The Recombinant Mouse ASAH2 Protein, CF from R&D Systems is derived from NS0. The Recombinant Mouse ASAH2 Protein, CF has been validated for the following applications: Enzyme Activity.

target :

ASAH2/N-acylsphingosine Amidohydrolase-2

category :

Proteins and Enzymes

buffer :

Supplied as a 0.2 ╡m filtered solution in MES, NaCl and Sodium Cholate.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

species :

Mouse

observed molecular weight :

113 kDa, reducing conditions

theoretical molecular weight :

81 kDa

gene symbol :

Asah2

details of functionality :

Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid. The specific activity is 3,000 pmol/min/╡g, as measured under the described conditions.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_061300

applications :

Enzyme Activity

source long :

Mouse myeloma cell line, NS0-derived mouse ASAH2/N-acylsphingosine Amidohydrolase-2 protein Thr34-Thr756, with an N-terminal 6-His tag Accession # NP_061300

source short :

NS0

USD :

477 USD

alt names :

ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2, BCDase, HNAC1, LCDase, Nacylsphingosine Amidohydrolase2, NCDase, N-CDase

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 ░C as supplied. 3 months, -70 ░C under sterile conditions after opening.

more info or order :

R&D Systems product webpage