Cultrex RGF Basement Membrane Extract, Type 2 | R&D Systems 3533-001-02 product information

product summary

company name :

R&D Systems

product type :

other

product name :

Cultrex RGF Basement Membrane Extract, Type 2

catalog :

3533-001-02

quantity :

1 mL

price :

62 USD

more info or order :

R&D Systems product webpage

citations: 21

Reference
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Simoneau C, Chen P, Xing G, Hayashi J, Chen I, Khalid M, et al. NF-κB inhibitor alpha controls SARS-CoV-2 infection in ACE2-overexpressing human airway organoids. Sci Rep. 2024;14:15351 pubmed publisher
Tavakoli N, Fong E, Coleman A, Huang Y, Bigger M, Doche M, et al. Merging Metabolic Modeling and Imaging for Screening Therapeutic Targets in Colorectal Cancer. bioRxiv. 2024;: pubmed publisher
Zou C, Shen J, Xu F, Ye Y, Wu Y, Xu S. Immunoreactive Microenvironment Modulator GBP5 Suppresses Ovarian Cancer Progression by Inducing Canonical Pyroptosis. J Cancer. 2024;15:3510-3530 pubmed publisher
Bannier H xe9 laou xeb t M, Korving J, Ma Z, Begthel H, Giladi A, Lamers M, et al. Human conjunctiva organoids to study ocular surface homeostasis and disease. Cell Stem Cell. 2024;31:227-243.e12 pubmed publisher
Geurts M, Gandhi S, Boretto M, Akkerman N, Derks L, van Son G, et al. One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids. Nat Commun. 2023;14:4998 pubmed publisher
Hogstrom J, Cruz K, Selfors L, Ward M, Mehta T, Kanarek N, et al. Simultaneous isolation of hormone receptor-positive breast cancer organoids and fibroblasts reveals stroma-mediated resistance mechanisms. J Biol Chem. 2023;299:105021 pubmed publisher
Niu Y, Ferreira Azevedo C, Li X, Kamali E, Haagen Nielsen O, Storgaard S xf8 rensen C, et al. Multiparametric and accurate functional analysis of genetic sequence variants using CRISPR-Select. Nat Genet. 2022;54:1983-1993 pubmed publisher
Beumer J, Puschhof J, Yengej F, Zhao L, Martínez Silgado A, Blotenburg M, et al. BMP gradient along the intestinal villus axis controls zonated enterocyte and goblet cell states. Cell Rep. 2022;38:110438 pubmed publisher
Huang L, Rong Y, Tang X, Yi K, Qi P, Hou J, et al. Engineered exosomes as an in situ DC-primed vaccine to boost antitumor immunity in breast cancer. Mol Cancer. 2022;21:45 pubmed publisher
Cosgrove N, Vare x161 lija D, Keelan S, Elangovan A, Atkinson J, Cocchiglia S, et al. Mapping molecular subtype specific alterations in breast cancer brain metastases identifies clinically relevant vulnerabilities. Nat Commun. 2022;13:514 pubmed publisher
Puschhof J, Pleguezuelos Manzano C, Martínez Silgado A, Akkerman N, Saftien A, Boot C, et al. Intestinal organoid cocultures with microbes. Nat Protoc. 2021;16:4633-4649 pubmed publisher
Bannier Hélaouët M, Post Y, Korving J, Trani Bustos M, Gehart H, Begthel H, et al. Exploring the human lacrimal gland using organoids and single-cell sequencing. Cell Stem Cell. 2021;28:1221-1232.e7 pubmed publisher
Busslinger G, Weusten B, Bogte A, Begthel H, Brosens L, Clevers H. Human gastrointestinal epithelia of the esophagus, stomach, and duodenum resolved at single-cell resolution. Cell Rep. 2021;34:108819 pubmed publisher
Puschhof J, Post Y, Beumer J, Kerkkamp H, Bittenbinder M, Vonk F, et al. Derivation of snake venom gland organoids for in vitro venom production. Nat Protoc. 2021;16:1494-1510 pubmed publisher
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product information

brand :

R&D Systems

master code :

3533-001-02

SKU :

3533-001-02

product name :

Cultrex RGF Basement Membrane Extract, Type 2

unit size :

1 mL

target :

Basement Membrane Extracts

category :

Cell Culture Products

buffer :

12 - 18 mg/mL.

dilution :

Bioactivity

details of functionality :

Tube Formation Assay - BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells. Gelling Assay - BME gels in less than 30 minutes at 37░C, and maintains the gelled form in culture medium for a minimum of 14 days at 37░C.

endotoxin note :

<8.0 EU per 1 ╡g of the protein by the LAL method.

applications :

Bioactivity

extended description :

For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract

USD :

62 USD

product details :

For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract

storage :

Product is stable for a minimum of 3 months from date of shipment when stored at -20 ░C in a manual defrost freezer. For optimal stability, store at -80 ░C. Avoid freeze-thaw cycles. Storage Buffer: DulbeccoÆs Modified EagleÆs medium without phenol red, with 10 ug/ml gentamicin sulfate. Sterility Testing: PathClear« - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 ░C for 14 days following USP sterility testing guidelines.

more info or order :

R&D Systems product webpage