Recombinant Human R-Spondin 3 Protein | R&D Systems 3500-RS-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human R-Spondin 3 Protein

catalog :

3500-RS-025

quantity :

25 ug

price :

377 USD

more info or order :

R&D Systems product webpage

citations: 14

Reference
Pizzolato G, Moparthi L, S xf6 derholm S, Cant xf9 C, Koch S. The oncogenic transcription factor FOXQ1 is a differential regulator of Wnt target genes. J Cell Sci. 2022;135: pubmed publisher
Dekkers J, van Vliet E, Sachs N, Rosenbluth J, Kopper O, Rebel H, et al. Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids. Nat Protoc. 2021;16:1936-1965 pubmed publisher
Beaurivage C, Kanapeckaite A, Loomans C, Erdmann K, Stallen J, Janssen R. Development of a human primary gut-on-a-chip to model inflammatory processes. Sci Rep. 2020;10:21475 pubmed publisher
Li X, Pan B, Song X, Li N, Zhao D, Li M, et al. Breast cancer organoids from a patient with giant papillary carcinoma as a high-fidelity model. Cancer Cell Int. 2020;20:86 pubmed publisher
Zhang M, Haughey M, Wang N, Blease K, Kapoun A, Couto S, et al. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs. PLoS ONE. 2020;15:e0229445 pubmed publisher
Boone P, Rochelle L, Ginzel J, Lubkov V, Roberts W, Nicholls P, et al. A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion. Nat Commun. 2019;10:5490 pubmed publisher
Park S, Cui J, Yu W, Wu L, Carmon K, Liu Q. Differential activities and mechanisms of the four R-spondins in potentiating Wnt/β-catenin signaling. J Biol Chem. 2018;293:9759-9769 pubmed publisher
Lebensohn A, Rohatgi R. R-spondins can potentiate WNT signaling without LGRs. elife. 2018;7: pubmed publisher
Zeve D, Millay D, Seo J, Graff J. Exercise-Induced Skeletal Muscle Adaptations Alter the Activity of Adipose Progenitor Cells. PLoS ONE. 2016;11:e0152129 pubmed publisher
Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee Hoskins C, et al. Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates Î²-Catenin Signaling and Tumorigenesis in Multiple Cancer Types. Cancer Res. 2016;76:713-23 pubmed publisher
Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, et al. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun. 2014;5:3149 pubmed publisher
Xie Y, Zamponi R, Charlat O, Ramones M, Swalley S, Jiang X, et al. Interaction with both ZNRF3 and LGR4 is required for the signalling activity of R-spondin. EMBO Rep. 2013;14:1120-6 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Hao H, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, et al. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature. 2012;485:195-200 pubmed publisher

image

image 1 :

Recombinant Human R-Spondin 3 Protein Bioactivity

product information

brand :

R&D Systems

master code :

3500-RS

SKU :

3500-RS-025

product name :

Recombinant Human R-Spondin 3 Protein

description :

The Recombinant Human R-Spondin 3 Protein from R&D Systems is derived from CHO. The Recombinant Human R-Spondin 3 Protein has been validated for the following applications: Bioactivity.

target :

R-Spondin 3

category :

Proteins and Enzymes

unit sizes :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

36-45 kDa, reducing conditions

theoretical molecular weight :

28.3 kDa

gene symbol :

RSPO3

details of functionality :

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.500-2.00 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

Q9BXY4

applications :

Bioactivity

source :

CHO-derived Recombinant Human R-Spondin 3 Protein

USD :

377 USD

alt names :

Cristin 1, CRISTIN1, FLJ14440, hPWTSR, hRspo3, Protein with TSP type-1 repeat, PWTSR, Roof plate-specific spondin-3, RSPO3, RSpondin 3, R-spondin 3 homolog (Xenopus laevis), R-spondin-3, Thrombospondin type-1 domain-containing protein 2, thrombospondin, type I, domain containing 2, THSD2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage