Recombinant Human R-Spondin 3 Protein | R&D Systems 3500-RS-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human R-Spondin 3 Protein

catalog :

3500-RS-025

quantity :

25 ug

price :

400 USD

citations: 18

Reference
Song X, Zhou Z, Elmezayen A, Wu R, Yu C, Gao B, et al. SRC kinase drives multidrug resistance induced by KRAS-G12C inhibition. Sci Adv. 2024;10:eadq4274 pubmed publisher
Nagano K, Yamana K, Saito H, Kiviranta R, Pedroni A, Raval D, et al. R-spondin 3 deletion induces Erk phosphorylation to enhance Wnt signaling and promote bone formation in the appendicular skeleton. elife. 2022;11: pubmed publisher
Pizzolato G, Moparthi L, S xf6 derholm S, Cant xf9 C, Koch S. The oncogenic transcription factor FOXQ1 is a differential regulator of Wnt target genes. J Cell Sci. 2022;135: pubmed publisher
Dekkers J, van Vliet E, Sachs N, Rosenbluth J, Kopper O, Rebel H, et al. Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids. Nat Protoc. 2021;16:1936-1965 pubmed publisher
Beaurivage C, Kanapeckaite A, Loomans C, Erdmann K, Stallen J, Janssen R. Development of a human primary gut-on-a-chip to model inflammatory processes. Sci Rep. 2020;10:21475 pubmed publisher
Loh N, Minchin J, Pinnick K, Verma M, Todorcević M, Denton N, et al. RSPO3 impacts body fat distribution and regulates adipose cell biology in vitro. Nat Commun. 2020;11:2797 pubmed publisher
Li X, Pan B, Song X, Li N, Zhao D, Li M, et al. Breast cancer organoids from a patient with giant papillary carcinoma as a high-fidelity model. Cancer Cell Int. 2020;20:86 pubmed publisher
Zhang M, Haughey M, Wang N, Blease K, Kapoun A, Couto S, et al. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs. PLoS ONE. 2020;15:e0229445 pubmed publisher
Boone P, Rochelle L, Ginzel J, Lubkov V, Roberts W, Nicholls P, et al. A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion. Nat Commun. 2019;10:5490 pubmed publisher
Park S, Cui J, Yu W, Wu L, Carmon K, Liu Q. Differential activities and mechanisms of the four R-spondins in potentiating Wnt/β-catenin signaling. J Biol Chem. 2018;293:9759-9769 pubmed publisher
Lebensohn A, Rohatgi R. R-spondins can potentiate WNT signaling without LGRs. elife. 2018;7: pubmed publisher
Zeve D, Millay D, Seo J, Graff J. Exercise-Induced Skeletal Muscle Adaptations Alter the Activity of Adipose Progenitor Cells. PLoS ONE. 2016;11:e0152129 pubmed publisher
Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee Hoskins C, et al. Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates Î²-Catenin Signaling and Tumorigenesis in Multiple Cancer Types. Cancer Res. 2016;76:713-23 pubmed publisher
Carmon K, Gong X, Yi J, Thomas A, Liu Q. RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. Proc Natl Acad Sci U S A. 2014;111:E1221-9 pubmed publisher
Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, et al. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun. 2014;5:3149 pubmed publisher
Xie Y, Zamponi R, Charlat O, Ramones M, Swalley S, Jiang X, et al. Interaction with both ZNRF3 and LGR4 is required for the signalling activity of R-spondin. EMBO Rep. 2013;14:1120-6 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Hao H, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, et al. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature. 2012;485:195-200 pubmed publisher

product information

master code :

3500-RS

SKU :

3500-RS-025

product name :

Recombinant Human R-Spondin 3 Protein

unit size :

25 ug

description :

The Recombinant Human R-Spondin 3 Protein from R&D Systems is derived from CHO. The Recombinant Human R-Spondin 3 Protein has been validated for the following applications: Bioactivity.

target :

R-Spondin 3

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS with BSA as a carrier protein.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

36-45 kDa, reducing conditions

theoretical molecular weight :

28.3 kDa

gene symbol :

RSPO3

details of functionality :

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.500-2.00 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

Q9BXY4

applications :

Bioactivity

source long :

Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 3 protein Gln22-His272

source short :

CHO

extended description :

Analyzed by SEC-MALS

USD :

400 USD

product details :

Analyzed by SEC-MALS

alt names :

Cristin 1, CRISTIN1, FLJ14440, hPWTSR, hRspo3, Protein with TSP type-1 repeat, PWTSR, Roof plate-specific spondin-3, RSPO3, RSpondin 3, R-spondin 3 homolog (Xenopus laevis), R-spondin-3, Thrombospondin type-1 domain-containing protein 2, thrombospondin, type I, domain containing 2, THSD2

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.