product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Mouse R-Spondin 1 Protein, CF
catalog :
3474-RS-050
quantity :
50 ug (also 250 ug, 1 mg)
price :
482 USD
more info or order :
citations: 68
Reference
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Zhang X, Bandyopadhyay S, Araujo L, Tong K, Flores J, Laubitz D, et al. Elevating EGFR-MAPK program by a nonconventional Cdc42 enhances intestinal epithelial survival and regeneration. JCI Insight. 2020;5: pubmed publisher
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Zhang M, Haughey M, Wang N, Blease K, Kapoun A, Couto S, et al. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs. PLoS ONE. 2020;15:e0229445 pubmed publisher
Xing T, Benderman L, Sabu S, Parker J, Yang J, Lu Q, et al. Tight Junction Protein Claudin-7 Is Essential for Intestinal Epithelial Stem Cell Self-Renewal and Differentiation. Cell Mol Gastroenterol Hepatol. 2020;9:641-659 pubmed publisher
Singh A, Hung Y, Shanahan M, Kanke M, Bonfini A, Dame M, et al. Enteroendocrine Progenitor Cell-Enriched miR-7 Regulates Intestinal Epithelial Proliferation in an Xiap-Dependent Manner. Cell Mol Gastroenterol Hepatol. 2020;9:447-464 pubmed publisher
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Meng Z, Gwag T, Sui Y, Park S, Zhou X, Zhou C. The atypical antipsychotic quetiapine induces hyperlipidemia by activating intestinal PXR signaling. JCI Insight. 2019;4: pubmed publisher
Chen F, Yang W, Huang X, Cao A, Bilotta A, Xiao Y, et al. Neutrophils Promote Amphiregulin Production in Intestinal Epithelial Cells through TGF-? and Contribute to Intestinal Homeostasis. J Immunol. 2018;: pubmed publisher
Smith N, Swain J, Davies P, Gallagher A, Parappilly M, Beach C, et al. Monoclonal Antibodies Reveal Dynamic Plasticity Between Lgr5- and Bmi1-Expressing Intestinal Cell Populations. Cell Mol Gastroenterol Hepatol. 2018;6:79-96 pubmed publisher
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Takahashi T, Shiraishi A, Murata J. The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice. Int J Mol Sci. 2018;19: pubmed publisher
Saito Y, Onishi N, Takami H, Seishima R, Inoue H, Hirata Y, et al. Development of a functional thyroid model based on an organoid culture system. Biochem Biophys Res Commun. 2018;497:783-789 pubmed publisher
Yui S, Azzolin L, Maimets M, Pedersen M, Fordham R, Hansen S, et al. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Cell Stem Cell. 2018;22:35-49.e7 pubmed publisher
Scavuzzo M, Yang D, Borowiak M. Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells. Sci Rep. 2017;7:10810 pubmed publisher
Moorefield E, Andres S, Blue R, Van Landeghem L, Mah A, Santoro M, et al. Aging effects on intestinal homeostasis associated with expansion and dysfunction of intestinal epithelial stem cells. Aging (Albany NY). 2017;9:1898-1915 pubmed publisher
Feng Q, Bonder E, Engevik A, Zhang L, Tyska M, Goldenring J, et al. Disruption of Rab8a and Rab11a causes formation of basolateral microvilli in neonatal enteropathy. J Cell Sci. 2017;130:2491-2505 pubmed publisher
Peck B, Mah A, Pitman W, Ding S, Lund P, Sethupathy P. Functional Transcriptomics in Diverse Intestinal Epithelial Cell Types Reveals Robust MicroRNA Sensitivity in Intestinal Stem Cells to Microbial Status. J Biol Chem. 2017;292:2586-2600 pubmed publisher
Attayek P, Ahmad A, Wang Y, Williamson I, Sims C, Magness S, et al. In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment. PLoS ONE. 2016;11:e0153795 pubmed publisher
Morrison G, Scognamiglio R, Trumpp A, Smith A. Convergence of cMyc and β-catenin on Tcf7l1 enables endoderm specification. EMBO J. 2016;35:356-68 pubmed publisher
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Calderon Gierszal E, Prins G. Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure. PLoS ONE. 2015;10:e0133238 pubmed publisher
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Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, et al. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis. PLoS ONE. 2015;10:e0124213 pubmed publisher
Moon C, Zhang W, Ren A, Arora K, Sinha C, Yarlagadda S, et al. Compartmentalized accumulation of cAMP near complexes of multidrug resistance protein 4 (MRP4) and cystic fibrosis transmembrane conductance regulator (CFTR) contributes to drug-induced diarrhea. J Biol Chem. 2015;290:11246-57 pubmed publisher
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McElroy S, Castle S, Bernard J, Almohazey D, Hunter C, Bell B, et al. The ErbB4 ligand neuregulin-4 protects against experimental necrotizing enterocolitis. Am J Pathol. 2014;184:2768-78 pubmed publisher
Fukuda M, Mizutani T, Mochizuki W, Matsumoto T, Nozaki K, Sakamaki Y, et al. Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon. Genes Dev. 2014;28:1752-7 pubmed publisher
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Takemura N, Kawasaki T, Kunisawa J, Sato S, Lamichhane A, Kobiyama K, et al. Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome. Nat Commun. 2014;5:3492 pubmed publisher
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Li J, Chai B, Zhang W, Fritze D, Zhang C, Mulholland M. LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats. Endocrinology. 2014;155:429-40 pubmed publisher
Jin L, Feng T, Zerda R, Chen C, Riggs A, Ku H. In vitro multilineage differentiation and self-renewal of single pancreatic colony-forming cells from adult C57BL/6 mice. Stem Cells Dev. 2014;23:899-909 pubmed publisher
Huynh D, Akcora D, Malaterre J, Chan C, Dai X, Bertoncello I, et al. CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response. PLoS ONE. 2013;8:e56951 pubmed publisher
Jin L, Feng T, Shih H, Zerda R, Luo A, Hsu J, et al. Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel. Proc Natl Acad Sci U S A. 2013;110:3907-12 pubmed publisher
Jarde T, Evans R, McQuillan K, Parry L, Feng G, Alvares B, et al. In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-OΔN89β-catenin system. Oncogene. 2013;32:883-93 pubmed publisher
Wong V, Stange D, Page M, Buczacki S, Wabik A, Itami S, et al. Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling. Nat Cell Biol. 2012;14:401-8 pubmed publisher
Tomaselli S, Megiorni F, Lin L, Mazzilli M, Gerrelli D, Majore S, et al. Human RSPO1/R-spondin1 is expressed during early ovary development and augments β-catenin signaling. PLoS ONE. 2011;6:e16366 pubmed publisher
image
image 1 :
R&D Systems 3474-RS-050 image 1
Recombinant Mouse R-Spondin 1 Protein Bioactivity
product information
brand :
R&D Systems, a Bio-Techne brand
catalog number base :
3474-RS
SKU :
3474-RS-050
product name :
Recombinant Mouse R-Spondin 1 Protein, CF
description :
The Recombinant Mouse R-Spondin 1 Protein, CF from R&D Systems is derived from E. coli. The Recombinant Mouse R-Spondin 1 Protein, CF has been validated for the following applications: Bioactivity.
target :
R-Spondin 1
unit size :
50 ug (also 250 ug, 1 mg)
category :
Proteins and Enzymes
buffer :
Lyophilized from a 0.2 µm filtered solution in PBS.
conjugate :
Unconjugated
purity :
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Mouse
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
theoretical molecular weight :
21 kDa
gene symbol :
Rspo1
details of functionality :
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
Q9Z132
applications :
Bioactivity
product keywords :
3474-RS, rmR-Spondin 1, mR-Spondin 1, carrier-free, carrier free, 3474RS, RSpondin 1, R-Spondin1, R Spondin 1, Cristin-3, Thrombospondin, Roof Plate-Specific Spondin-1, R-Spondin Homolog, CRISTIN3, HRspo1, RSPO
source :
E. coli-derived Recombinant Mouse R-Spondin 1 Protein, CF
USD :
477
USD 2025 :
482 USD
alt names :
Cristin 3, CRISTIN3, FLJ40906Roof plate-specific spondin-1, hRspo1, roof plate-specific spondin, RSPO, RSPO1, RSPONDIN, RSpondin 1, R-spondin homolog (Xenopus laevis), R-spondin1, R-spondin-1
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.