Cultrex 3-D Culture Matrix RGF Basement Membrane Extract | R&D Systems 3445-010-01 product information

product summary

company name :

R&D Systems

product type :

other

product name :

Cultrex 3-D Culture Matrix RGF Basement Membrane Extract

catalog :

3445-010-01

quantity :

10 mL (2 x 5 mL)

price :

483 USD

more info or order :

R&D Systems product webpage

citations: 21

Reference
Liu Q, Qi J, Li W, Tian X, Zhang J, Liu F, et al. Therapeutic effect and transcriptome-methylome characteristics of METTL3 inhibition in liver hepatocellular carcinoma. Cancer Cell Int. 2023;23:298 pubmed publisher
Boccanegra B, Mantuano P, Conte E, Cerchiara A, Tulimiero L, Quarta R, et al. LKB1 signaling is altered in skeletal muscle of a Duchenne muscular dystrophy mouse model. Dis Model Mech. 2023;16: pubmed publisher
Del Nero M, Colombo A, Garbujo S, Baioni C, Barbieri L, Innocenti M, et al. Advanced Cell Culture Models Illuminate the Interplay between Mammary Tumor Cells and Activated Fibroblasts. Cancers (Basel). 2023;15: pubmed publisher
Matkovic Leko I, Schneider R, Thimraj T, Schrode N, Beitler D, Liu H, et al. A distal lung organoid model to study interstitial lung disease, viral infection and human lung development. Nat Protoc. 2023;18:2283-2312 pubmed publisher
Doffe F, Bonini F, Lakis E, Terry S, Chouaib S, Savagner P. Designing Organoid Models to Monitor Cancer Progression, Plasticity and Resistance: The Right Set Up for the Right Question. Cancers (Basel). 2022;14: pubmed publisher
Kadamb R, Leibovitch B, Farias E, Dahiya N, Suryawanshi H, Bansal N, et al. Invasive phenotype in triple negative breast cancer is inhibited by blocking SIN3A-PF1 interaction through KLF9 mediated repression of ITGA6 and ITGB1. Transl Oncol. 2022;16:101320 pubmed publisher
Lattke M, Goldstone R, Ellis J, Boeing S, Jurado Arjona J, Marichal N, et al. Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture. Nat Commun. 2021;12:4335 pubmed publisher
Cortés Troncoso J, Jang S, Perez P, Hidalgo J, Ikeuchi T, Greenwell Wild T, et al. T cell exosome-derived miR-142-3p impairs glandular cell function in Sjögren's syndrome. JCI Insight. 2020;5: pubmed publisher
Xue Y, Meehan B, Macdonald E, Venneti S, Wang X, Witkowski L, et al. CDK4/6 inhibitors target SMARCA4-determined cyclin D1 deficiency in hypercalcemic small cell carcinoma of the ovary. Nat Commun. 2019;10:558 pubmed publisher
Das A, Huang G, Bonkowski M, Longchamp A, Li C, Schultz M, et al. Impairment of an Endothelial NAD+-H2S Signaling Network Is a Reversible Cause of Vascular Aging. Cell. 2018;173:74-89.e20 pubmed publisher
Arasu U, Kärnä R, Härkönen K, Oikari S, Koistinen A, Kroger H, et al. Human mesenchymal stem cells secrete hyaluronan-coated extracellular vesicles. Matrix Biol. 2017;64:54-68 pubmed publisher
Martinez Marin D, Jarvis C, Nelius T, de Riese W, Volpert O, Filleur S. PEDF increases the tumoricidal activity of macrophages towards prostate cancer cells in vitro. PLoS ONE. 2017;12:e0174968 pubmed publisher
Chalasani A, Ji K, Sameni M, Mazumder S, Xu Y, Moin K, et al. Live-Cell Imaging of Protease Activity: Assays to Screen Therapeutic Approaches. Methods Mol Biol. 2017;1574:215-225 pubmed publisher
Okere B, Alviano F, Costa R, Quaglino D, Ricci F, Dominici M, et al. In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids. Int J Immunopathol Pharmacol. 2015;28:390-402 pubmed publisher
Jechlinger M. Organotypic culture of untransformed and tumorigenic primary mammary epithelial cells. Cold Spring Harb Protoc. 2015;2015:457-61 pubmed publisher
Parvani J, Gujrati M, Mack M, Schiemann W, Lu Z. Silencing β3 Integrin by Targeted ECO/siRNA Nanoparticles Inhibits EMT and Metastasis of Triple-Negative Breast Cancer. Cancer Res. 2015;75:2316-2325 pubmed publisher
Sottnik J, Dai J, Zhang H, Campbell B, Keller E. Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases. Cancer Res. 2015;75:2151-8 pubmed publisher
Rodriguez Teja M, Gronau J, Minamidate A, Darby S, Gaughan L, Robson C, et al. Survival Outcome and EMT Suppression Mediated by a Lectin Domain Interaction of Endo180 and CD147. Mol Cancer Res. 2015;13:538-47 pubmed publisher
Chou C, Lee K, Lai I, Wang D, Mo X, Kulp S, et al. AMPK reverses the mesenchymal phenotype of cancer cells by targeting the Akt-MDM2-Foxo3a signaling axis. Cancer Res. 2014;74:4783-95 pubmed publisher
Grugan K, McCabe F, Kinder M, Greenplate A, Harman B, Ekert J, et al. Tumor-associated macrophages promote invasion while retaining Fc-dependent anti-tumor function. J Immunol. 2012;189:5457-66 pubmed publisher
Jahchan N, Wang D, Bissell M, Luo K. SnoN regulates mammary gland alveologenesis and onset of lactation by promoting prolactin/Stat5 signaling. Development. 2012;139:3147-56 pubmed publisher

product information

brand :

R&D Systems

master code :

3445-010-01

SKU :

3445-010-01

product name :

Cultrex 3-D Culture Matrix RGF Basement Membrane Extract

unit size :

10 mL (2 x 5 mL)

target :

Basement Membrane Extracts

category :

Cell Culture Products

buffer :

14 - 16 mg/mL.

dilution :

Bioactivity

species :

Multi-Species

details of functionality :

Functional assays: Tube formation assay - BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells. 3D Culture - Basement Membrane Extract promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) or human prostate (PC-3) into acinar structures. Gelling assay: BME gels in less than 30 minutes at 37░C, and maintains the gelled form in culture medium for a minimum of 14 days at 37░C.

endotoxin note :

<8.0 EU per 1 ╡g of the protein by the LAL method.

applications :

Bioactivity

extended description :

Reduced Growth Factor Basement Membrane Extract for 3-D Cell Culture

USD :

483 USD

product details :

Reduced Growth Factor Basement Membrane Extract for 3-D Cell Culture

storage :

Product is stable for a minimum of 3 months from date of shipment when stored at -20 ░C in a manual defrost freezer. For optimal stability, store at -80 ░C. Avoid freeze-thaw cycles. Storage Buffer: DulbeccoÆs Modified EagleÆs medium without phenol red, with 10 ╡g/ml gentamicin sulfate. Sterility Testing: PathClear« - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 ░C for 14 days following USP sterility testing guidelines.

more info or order :

R&D Systems product webpage