Cultrex 3-D Culture Matrix RGF Basement Membrane Extract | R&D Systems 3445-005-01 product information

product summary

company name :

R&D Systems

product type :

other

product name :

Cultrex 3-D Culture Matrix RGF Basement Membrane Extract

catalog :

3445-005-01

quantity :

5 mL

price :

279 USD

more info or order :

R&D Systems product webpage

citations: 24

Reference
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Giobbe G, Crowley C, Luni C, Campinoti S, Khedr M, Kretzschmar K, et al. Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture. Nat Commun. 2019;10:5658 pubmed publisher
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product information

brand :

R&D Systems

master code :

3445-005-01

SKU :

3445-005-01

product name :

Cultrex 3-D Culture Matrix RGF Basement Membrane Extract

unit size :

5 mL

target :

Basement Membrane Extracts

category :

Cell Culture Products

buffer :

14 - 16 mg/mL.

dilution :

Bioactivity

species :

Multi-Species

details of functionality :

Functional assays: Tube formation assay - BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells. 3D Culture - Basement Membrane Extract promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) or human prostate (PC-3) into acinar structures. Gelling assay: BME gels in less than 30 minutes at 37░C, and maintains the gelled form in culture medium for a minimum of 14 days at 37░C.

endotoxin note :

<8.0 EU per 1 ╡g of the protein by the LAL method.

applications :

Bioactivity

extended description :

Reduced Growth Factor Basement Membrane Extract for 3-D Cell Culture

USD :

279 USD

product details :

Reduced Growth Factor Basement Membrane Extract for 3-D Cell Culture

storage :

Product is stable for a minimum of 3 months from date of shipment when stored at -20 ░C in a manual defrost freezer. For optimal stability, store at -80 ░C. Avoid freeze-thaw cycles. Storage Buffer: DulbeccoÆs Modified EagleÆs medium without phenol red, with 10 ╡g/ml gentamicin sulfate. Sterility Testing: PathClear« - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 ░C for 14 days following USP sterility testing guidelines.

more info or order :

R&D Systems product webpage