product summary
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company name :
R&D Systems
product type :
other
product name :
Cultrex 3-D Culture Matrix RGF Basement Membrane Extract
catalog :
3445-001-01
quantity :
1 mL
price :
63 USD
more info or order :
citations: 3
Reference
Parvani J, Gujrati M, Mack M, Schiemann W, Lu Z. Silencing β3 Integrin by Targeted ECO/siRNA Nanoparticles Inhibits EMT and Metastasis of Triple-Negative Breast Cancer. Cancer Res. 2015;75:2316-2325 pubmed publisher
Sottnik J, Dai J, Zhang H, Campbell B, Keller E. Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases. Cancer Res. 2015;75:2151-8 pubmed publisher
Chou C, Lee K, Lai I, Wang D, Mo X, Kulp S, et al. AMPK reverses the mesenchymal phenotype of cancer cells by targeting the Akt-MDM2-Foxo3a signaling axis. Cancer Res. 2014;74:4783-95 pubmed publisher
product information
master code :
3445-001-01
SKU :
3445-001-01
product name :
Cultrex 3-D Culture Matrix RGF Basement Membrane Extract
unit size :
1 mL
target :
Basement Membrane Extracts
category :
Cell Culture Products
buffer :
14 - 16 mg/mL.
species :
Multi-Species
details of functionality :
Functional assays: Tube formation assay - BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells. 3D Culture - Basement Membrane Extract promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) or human prostate (PC-3) into acinar structures. Gelling assay: BME gels in less than 30 minutes at 37░C, and maintains the gelled form in culture medium for a minimum of 14 days at 37░C.
endotoxin note :
<8.0 EU per 1 ╡g of the protein by the LAL method.
applications :
Bioactivity
extended description :
Reduced Growth Factor Basement Membrane Extract for 3-D Cell Culture
USD :
63 USD
product details :
Reduced Growth Factor Basement Membrane Extract for 3-D Cell Culture
storage :
Product is stable for a minimum of 3 months from date of shipment when stored at -20 ░C in a manual defrost freezer. For optimal stability, store at -80 ░C. Avoid freeze-thaw cycles. Storage Buffer: DulbeccoÆs Modified EagleÆs medium without phenol red, with 10 ug/ml gentamicin sulfate. Sterility Testing: PathClear« - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 ░C for 14 days following USP sterility testing guidelines.
more info or order :
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.