Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF | R&D Systems 342-CD-200 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

catalog :

342-CD-200

quantity :

200 ug

price :

449 USD

more info or order :

R&D Systems product webpage

citations: 7

Reference
Hanson A, Elpek K, Duong E, Shallberg L, Fan M, Johnson C, et al. ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models. PLoS ONE. 2020;15:e0239595 pubmed publisher
Ramagopal U, Liu W, Garrett Thomson S, Bonanno J, Yan Q, Srinivasan M, et al. Structural basis for cancer immunotherapy by the first-in-class checkpoint inhibitor ipilimumab. Proc Natl Acad Sci U S A. 2017;114:E4223-E4232 pubmed publisher
Haanstra K, Dijkman K, Bashir N, Bauer J, Mary C, Poirier N, et al. Selective blockade of CD28-mediated T cell costimulation protects rhesus monkeys against acute fatal experimental autoimmune encephalomyelitis. J Immunol. 2015;194:1454-66 pubmed publisher
Kowalczyk A, D Souza C, Zhang L. Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3. Eur J Immunol. 2014;44:1143-55 pubmed publisher
Sakthivel P, Shively V, Kakoulidou M, Pearce W, Lefvert A. The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J Intern Med. 2007;261:399-407 pubmed
Neuber K, Mähnss B, Hubner C, Gergely H, Weichenthal M. Autoantibodies against CD28 are associated with atopic diseases. Clin Exp Immunol. 2006;146:262-9 pubmed
Lazetic S, Leong S, Chang J, Ong R, Dawes G, Punnonen J. Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells. J Biol Chem. 2002;277:38660-8 pubmed

product information

brand :

R&D Systems

master code :

342-CD

SKU :

342-CD-200

product name :

Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

description :

The Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF from R&D Systems is derived from NS0. The Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF has been validated for the following applications: Bioactivity.

target :

CD28

category :

Proteins and Enzymes

unit sizes :

200 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human, Cynomolgus Monkey, Rhesus Macaque

observed molecular weight :

60-70 kDa, reducing conditions

theoretical molecular weight :

42.2 kDa (monomer)

gene symbol :

CD28

details of functionality :

Measured by its binding ability in a functional ELISA. When Recombinant Human/Cynomolgus Monkey/Rhesus Macaque CD28 Fc Chimera is coated at 1 µg/mL (100 µL/well), the concentration of Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1 ) that produces 50% of the optimal binding response is approximately 0.05-0.3 µg/mL.

endotoxin note :

<0.10 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

P10747

applications :

Bioactivity

source :

NS0-derived Recombinant Human/Cynomolgus/Rhesus Macaque CD28 Fc, CF

USD :

449 USD

alt names :

CD28, CD28 antigen, CD28 antigen (Tp44), CD28 molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage