Recombinant Human Tenascin C Protein, CF | R&D Systems 3358-TC-050 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Tenascin C Protein, CF

catalog :

3358-TC-050

quantity :

50 ug

price :

509 USD

more info or order :

R&D Systems product webpage

citations: 16

Reference
Sun Z, Chen Z, Yin M, Wu X, Guo B, Cheng X, et al. Harnessing developmental dynamics of spinal cord extracellular matrix improves regenerative potential of spinal cord organoids. Cell Stem Cell. 2024;31:772-787.e11 pubmed publisher
Cai X, Han M, Lou F, Sun Y, Yin Q, Sun L, et al. Tenascin C+ papillary fibroblasts facilitate neuro-immune interaction in a mouse model of psoriasis. Nat Commun. 2023;14:2004 pubmed publisher
Cadamuro M, Sarcognato S, Camerotto R, Girardi N, Lasagni A, Zanus G, et al. Intrahepatic Cholangiocarcinoma Developing in Patients with Metabolic Syndrome Is Characterized by Osteopontin Overexpression in the Tumor Stroma. Int J Mol Sci. 2023;24: pubmed publisher
Umemoto A, Kuwada T, Murata K, Shiokawa M, Ota S, Murotani Y, et al. Identification of anti-citrullinated osteopontin antibodies and increased inflammatory response by enhancement of osteopontin binding to fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther. 2023;25:25 pubmed publisher
Kim S, Min S, Choi Y, Jo S, Jung J, Han K, et al. Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids. Nat Commun. 2022;13:1692 pubmed publisher
He C, Jiang B, Wang M, Ren P, Murtada S, Caulk A, et al. mTOR inhibition prevents angiotensin II-induced aortic rupture and pseudoaneurysm but promotes dissection in Apoe-deficient mice. JCI Insight. 2022;7: pubmed publisher
Takeo M, Asakawa K, Toyoshima K, Ogawa M, Tong J, Irie T, et al. Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration. Sci Rep. 2021;11:1173 pubmed publisher
Baskaran J, Weldy A, Guarin J, Munoz G, Shpilker P, Kotlik M, et al. Cell shape, and not 2D migration, predicts extracellular matrix-driven 3D cell invasion in breast cancer. APL Bioeng. 2020;4:026105 pubmed publisher
Hawkins A, Julian C, Konzen S, Treichel S, Lawlor E, Bailey K. Microenvironmental Factors Drive Tenascin C and Src Cooperation to Promote Invadopodia Formation in Ewing Sarcoma. Neoplasia. 2019;21:1063-1072 pubmed publisher
Lee J, Berry C, Kim K, Sen P, Kim T, Carrer A, et al. Acetyl-CoA promotes glioblastoma cell adhesion and migration through Ca2+-NFAT signaling. Genes Dev. 2018;32:497-511 pubmed publisher
Becerril S, Rodriguez A, Catalan V, Méndez Giménez L, Ramirez B, Sainz N, et al. Targeted disruption of the iNOS gene improves adipose tissue inflammation and fibrosis in leptin-deficient ob/ob mice: role of tenascin C. Int J Obes (Lond). 2018;42:1458-1470 pubmed publisher
Sarkar S, Mirzaei R, Zemp F, Wei W, Senger D, Robbins S, et al. Activation of NOTCH Signaling by Tenascin-C Promotes Growth of Human Brain Tumor-Initiating Cells. Cancer Res. 2017;77:3231-3243 pubmed publisher
Park H, Park J, Lee H, Kim S, Yun J, Kim M, et al. Integrins functioning in uterine endometrial stromal and epithelial cells in estrus. Reproduction. 2017;153:351-360 pubmed publisher
Carpenter P, Sivadas P, Hua S, Xiao C, Gutierrez A, Ngo T, et al. Migration of breast cancer cell lines in response to pulmonary laminin 332. Cancer Med. 2017;6:220-234 pubmed publisher
Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, et al. Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol. 2015;194:1039-46 pubmed publisher
Nakamura Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, et al. Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration. Blood. 2012;119:5429-37 pubmed publisher

product information

master code :

3358-TC

SKU :

3358-TC-050

product name :

Recombinant Human Tenascin C Protein, CF

unit size :

50 ug

description :

The Recombinant Human Tenascin C Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Tenascin C Protein, CF has been validated for the following applications: Bioactivity.

target :

Tenascin C

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS.

conjugate :

Unconjugated

purity :

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

97 kDa, reducing conditions

theoretical molecular weight :

65.3 kDa

gene symbol :

TNC

details of functionality :

Measured by the ability of the immobilized protein to block Fibronectin-mediated adhesion of NIH-3T3 mouse embryonic fibroblast cells. rhTenascin-C immobilized at 15 ╡g/mL, in the presence of 0.1 ╡g/mL human Fibronectin, will block approximately 70%-90% NIH3/T3 cell adhesion (5 x 104 cells/well, 100 ╡L/well).

endotoxin note :

<0.10 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

NP_002151

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human Tenascin C protein Gly23-Pro625, with a C-terminal 6-His tag

source short :

NS0

USD :

509 USD

alt names :

150-225, Cytotactin, Glioma-associated-extracellular matrix antigen, GMEM, GP 150-225, Hexabrachion, hexabrachion (tenascin C, cytotactin), hexabrachion (tenascin), HXB, HXBcytotactin, JI, MGC167029, Myotendinous antigen, neuronectin, tenascin, tenascin C, Tenascin J1, Tenascin-C, tenascin-C isoform 14/AD1/16, TNC, TN-C, TNGP

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage