product summary
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company name :
R&D Systems
product type :
protein
product name :
Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF
catalog :
3345-PS-050
quantity :
50 ug
price :
388 USD
more info or order :
R&D Systems product webpage
citations: 16
Reference
Yasunaga A, Li I. Quantification of fast molecular adhesion by fluorescence footprinting. Sci Adv. 2021;7: pubmed publisher
LAM F, Brown C, Valladolid C, Emebo D, Palzkill T, Cruz M. The vimentin rod domain blocks P-selectin-P-selectin glycoprotein ligand 1 interactions to attenuate leukocyte adhesion to inflamed endothelium. PLoS ONE. 2020;15:e0240164 pubmed publisher
Mehta N, Maddineni S, Kelly R, Lee R, Hunter S, Silberstein J, et al. An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA. Sci Rep. 2020;10:15171 pubmed publisher
Malaker S, Pedram K, Ferracane M, Bensing B, Krishnan V, Pett C, et al. The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins. Proc Natl Acad Sci U S A. 2019;116:7278-7287 pubmed publisher
Lavoie S, Dumas E, Vulesevic B, Neagoe P, White M, Sirois M. Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities. J Immunol. 2018;200:3801-3813 pubmed publisher
Lam F, Da Q, Guillory B, Cruz M. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium. J Immunol. 2018;200:1718-1726 pubmed publisher
Pepin M, Mezouar S, Pegon J, Muczynski V, Adam F, Bianchini E, et al. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity. Sci Rep. 2016;6:37953 pubmed publisher
Ku Z, Ye X, Shi J, Wang X, Liu Q, Huang Z. Single Neutralizing Monoclonal Antibodies Targeting the VP1 GH Loop of Enterovirus 71 Inhibit both Virus Attachment and Internalization during Viral Entry. J Virol. 2015;89:12084-95 pubmed publisher
Gutiérrez D, Pardo M, Montero D, Oñate A, Farfán M, Ruiz Pérez F, et al. TleA, a Tsh-like autotransporter identified in a human enterotoxigenic Escherichia coli strain. Infect Immun. 2015;83:1893-903 pubmed publisher
Ayala Lujan J, Vijayakumar V, Gong M, Smith R, Santiago A, Ruiz Perez F. Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes. PLoS ONE. 2014;9:e107920 pubmed publisher
Riese S, Kuehne C, Tedder T, Hallmann R, Hohenester E, Buscher K. Heterotropic modulation of selectin affinity by allosteric antibodies affects leukocyte rolling. J Immunol. 2014;192:1862-9 pubmed publisher
Banks J, Herman C, Bailey R. Bromelain decreases neutrophil interactions with P-selectin, but not E-selectin, in vitro by proteolytic cleavage of P-selectin glycoprotein ligand-1. PLoS ONE. 2013;8:e78988 pubmed publisher
Deban L, Russo R, Sironi M, Moalli F, Scanziani M, Zambelli V, et al. Regulation of leukocyte recruitment by the long pentraxin PTX3. Nat Immunol. 2010;11:328-34 pubmed publisher
Wu Z, Ethen C, Larson S, Prather B, Jiang W. A versatile polyacrylamide gel electrophoresis based sulfotransferase assay. BMC Biotechnol. 2010;10:11 pubmed publisher
Baker H, Basu I, Chung M, Caradoc Davies T, Fraser J, Baker E. Crystal structures of the staphylococcal toxin SSL5 in complex with sialyl Lewis X reveal a conserved binding site that shares common features with viral and bacterial sialic acid binding proteins. J Mol Biol. 2007;374:1298-308 pubmed
Simonis D, Fritzsche J, Alban S, Bendas G. Kinetic analysis of heparin and glucan sulfates binding to P-selectin and its impact on the general understanding of selectin inhibition. Biochemistry. 2007;46:6156-64 pubmed
image
image 1 :
R&D Systems 3345-PS-050 image 1
Recombinant Human PSGL-1/CD162 Fc Chimera Protein Bioactivity
product information
brand :
R&D Systems
master code :
3345-PS
SKU :
3345-PS-050
product name :
Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF
unit size :
50 ug
description :
Potent and selective MCH1 antagonist
target :
PSGL-1/CD162
category :
Proteins and Enzymes
alt names :
CD162, CD162 antigen, P-selectin glycoprotein ligand 1, PSGL1, PSGL-1cutaneous lymphocyte-associated associated antigen, selectin P ligandCLA, SELPLG
buffer :
Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
conjugate :
Unconjugated
all available conjugate :
Unconjugated
purity :
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
species :
Human
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
observed molecular weight :
100-150 kDa, reducing conditions
theoretical molecular weight :
52.6 kDa (monomer)
gene symbol :
SELPLG
details of functionality :
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is 0.04-0.32 µg/mL.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
accessionNumbers :
AAC50061
applications :
Bioactivity
source :
CHO-derived Recombinant Human PSGL-1/CD162 Fc Chimera Protein, CF
USD :
359
Jul-22 :
388 USD
sizes available :
50 ug
more info or order :
R&D Systems product webpage
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.