Recombinant Human R-Spondin 2 Protein, CF | R&D Systems 3266-RS-025/CF product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human R-Spondin 2 Protein, CF

catalog :

3266-RS-025/CF

quantity :

25 ug

price :

400 USD

more info or order :

R&D Systems product webpage

citations: 18

Reference
Sun R, He L, Lee H, Glinka A, Andresen C, Hübschmann D, et al. RSPO2 inhibits BMP signaling to promote self-renewal in acute myeloid leukemia. Cell Rep. 2021;36:109559 pubmed publisher
Wang C, Li Y, Jia L, Kim J, Li J, Deng P, et al. CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillance. Cell Stem Cell. 2021;28:1597-1613.e7 pubmed publisher
Zhu M, Iwano T, Takeda S. Fallopian Tube Basal Stem Cells Reproducing the Epithelial Sheets In Vitro-Stem Cell of Fallopian Epithelium. Biomolecules. 2020;10: pubmed publisher
Zhang M, Haughey M, Wang N, Blease K, Kapoun A, Couto S, et al. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs. PLoS ONE. 2020;15:e0229445 pubmed publisher
Haas M, Gómez Vázquez J, Sun D, Tran H, Brislinger M, Tasca A, et al. ΔN-Tp63 Mediates Wnt/β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia. Cell Rep. 2019;28:3338-3352.e6 pubmed publisher
Park S, Cui J, Yu W, Wu L, Carmon K, Liu Q. Differential activities and mechanisms of the four R-spondins in potentiating Wnt/β-catenin signaling. J Biol Chem. 2018;293:9759-9769 pubmed publisher
Szemes M, Greenhough A, Melegh Z, Malik S, Yuksel A, Catchpoole D, et al. Wnt Signalling Drives Context-Dependent Differentiation or Proliferation in Neuroblastoma. Neoplasia. 2018;20:335-350 pubmed publisher
Lebensohn A, Rohatgi R. R-spondins can potentiate WNT signaling without LGRs. elife. 2018;7: pubmed publisher
Kang Y, Kim J, Kim K, Chang J, Jung M, Lee J, et al. Upregulation of RSPO2-GPR48/LGR4 signaling in papillary thyroid carcinoma contributes to tumor progression. Oncotarget. 2017;8:114980-114994 pubmed publisher
van Andel H, Ren Z, Koopmans I, Joosten S, Kocemba K, de Lau W, et al. Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins. Proc Natl Acad Sci U S A. 2017;114:376-381 pubmed publisher
Nakashima H, Ohkawara B, Ishigaki S, Fukudome T, Ito K, Tsushima M, et al. R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5. Sci Rep. 2016;6:28512 pubmed publisher
Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee Hoskins C, et al. Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates Î²-Catenin Signaling and Tumorigenesis in Multiple Cancer Types. Cancer Res. 2016;76:713-23 pubmed publisher
Carmon K, Gong X, Yi J, Thomas A, Liu Q. RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling. Proc Natl Acad Sci U S A. 2014;111:E1221-9 pubmed publisher
Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, et al. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun. 2014;5:3149 pubmed publisher
Xie Y, Zamponi R, Charlat O, Ramones M, Swalley S, Jiang X, et al. Interaction with both ZNRF3 and LGR4 is required for the signalling activity of R-spondin. EMBO Rep. 2013;14:1120-6 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Hao H, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, et al. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner. Nature. 2012;485:195-200 pubmed publisher
Chua A, Ma D, Gan S, Fu Z, Han H, Song C, et al. The role of R-spondin2 in keratinocyte proliferation and epidermal thickening in keloid scarring. J Invest Dermatol. 2011;131:644-54 pubmed publisher

image

image 1 :

Recombinant Human R-Spondin 2 Protein Bioactivity

product information

brand :

R&D Systems, a Bio-Techne brand

catalog number base :

3266-RS/CF

SKU :

3266-RS-025/CF

product name :

Recombinant Human R-Spondin 2 Protein, CF

description :

The Recombinant Human R-Spondin 2 Protein, CF from R&D Systems is derived from NS0. The Recombinant Human R-Spondin 2 Protein, CF has been validated for the following applications: Bioactivity.

target :

R-Spondin 2

unit size :

25 ug

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

species :

Human

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

observed molecular weight :

25-29 kDa, under reducing conditions.

theoretical molecular weight :

22 kDa

gene symbol :

RSPO2

details of functionality :

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 0.7-2.8 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.

endotoxin note :

<0.01 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

NP_848660.2

applications :

Bioactivity

product keywords :

3266-RS, rhR-Spondin 2, hR-Spondin 2, carrier-free, carrier free, 3266RS/CF, RSpondin 2, R-Spondin2, Thrombospondin

source :

NS0-derived Recombinant Human R-Spondin 2 Protein, CF

USD :

396

USD 2025 :

400 USD

alt names :

Cristin 2, CRISTIN2, hRspo2, MGC35555, MGC43342, Roof plate-specific spondin-2, RSPO2, RSpondin 2, R-spondin 2 homolog (Xenopus laevis), R-spondin-2

more info or order :

R&D Systems product webpage