Recombinant Human Semaphorin 3E Protein, CF | R&D Systems 3239-S3B-025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Semaphorin 3E Protein, CF

catalog :

3239-S3B-025

quantity :

25 ug

price :

578 USD

citations: 6

Reference
Chen L, Yuan X, He Y, Fan Z, Guan Y, Li Q, et al. The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma Model. J Immunol Res. 2023;2023:6459234 pubmed publisher
Haider M, Saito H, Zarrer J, Uzhunnumpuram K, Nagarajan S, Kari V, et al. Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment. Breast Cancer Res. 2020;22:34 pubmed publisher
Aghajanian H, Cho Y, Manderfield L, Herling M, Gupta M, Ho V, et al. Coronary vasculature patterning requires a novel endothelial ErbB2 holoreceptor. Nat Commun. 2016;7:12038 pubmed publisher
Kusuhara S, Fukushima Y, Fukuhara S, Jakt L, Okada M, Shimizu Y, et al. Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells. PLoS ONE. 2012;7:e45858 pubmed publisher
Meda C, Molla F, De Pizzol M, Regano D, Maione F, Capano S, et al. Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages. J Immunol. 2012;188:4081-92 pubmed publisher
Fukushima Y, Okada M, Kataoka H, Hirashima M, Yoshida Y, Mann F, et al. Sema3E-PlexinD1 signaling selectively suppresses disoriented angiogenesis in ischemic retinopathy in mice. J Clin Invest. 2011;121:1974-85 pubmed publisher

product information

master code :

3239-S3B

SKU :

3239-S3B-025

product name :

Recombinant Human Semaphorin 3E Protein, CF

unit size :

25 ug

description :

The Recombinant Human Semaphorin 3E Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Semaphorin 3E Protein, CF has been validated for the following applications: Bioactivity.

target :

Semaphorin 3E

category :

Proteins and Enzymes

buffer :

Lyophilized from a 0.2 ╡m filtered solution in PBS, NaCl and Tween« 20 with Trehalose.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.

species :

Human

observed molecular weight :

85-100 kDa, 66 kDa and 25 kDa, reducing conditions

theoretical molecular weight :

88 kDa

gene symbol :

SEMA3E

details of functionality :

Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. etáal . (2010) Circ. Res. 106 :391. The ED50 for this effect is 0.04-0.24 ╡g/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin D1 (Catalog # 4160-PD ) is coated at 5 ╡g/mL, Recombinant Human Semaphorin 3E binds with an apparent Kd 0.4 nM.

endotoxin note :

<1.0 EU per 1 ╡g of the protein by the LAL method.

accessionNumbers :

O15041

applications :

Bioactivity

source long :

Mouse myeloma cell line, NS0-derived human Semaphorin 3E protein His24-Ser775 (Arg557Ala and Arg560Ala), with an N-terminal 10-His tag

source short :

NS0

USD :

578 USD

alt names :

(semaphorin) 3E, coll-5, KIAA0331M-SEMAH, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, Sema3E, SEMAH, SEMAHM-SemaK, semaphorin-3E

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ░C as supplied. 1 month, 2 to 8 ░C under sterile conditions after reconstitution. 3 months, -20 to -70 ░C under sterile conditions after reconstitution.