Recombinant Human Semaphorin 3E Protein, CF | R&D Systems 3239-S3B-025 product information

product summary

company name :

R&D Systems

product type :

protein

product name :

Recombinant Human Semaphorin 3E Protein, CF

catalog :

3239-S3B-025

quantity :

25 ug

price :

509 USD

more info or order :

R&D Systems product webpage

citations: 5

Reference
Haider M, Saito H, Zarrer J, Uzhunnumpuram K, Nagarajan S, Kari V, et al. Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment. Breast Cancer Res. 2020;22:34 pubmed publisher
Aghajanian H, Cho Y, Manderfield L, Herling M, Gupta M, Ho V, et al. Coronary vasculature patterning requires a novel endothelial ErbB2 holoreceptor. Nat Commun. 2016;7:12038 pubmed publisher
Kusuhara S, Fukushima Y, Fukuhara S, Jakt L, Okada M, Shimizu Y, et al. Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothelial cells. PLoS ONE. 2012;7:e45858 pubmed publisher
Meda C, Molla F, De Pizzol M, Regano D, Maione F, Capano S, et al. Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages. J Immunol. 2012;188:4081-92 pubmed publisher
Fukushima Y, Okada M, Kataoka H, Hirashima M, Yoshida Y, Mann F, et al. Sema3E-PlexinD1 signaling selectively suppresses disoriented angiogenesis in ischemic retinopathy in mice. J Clin Invest. 2011;121:1974-85 pubmed publisher

product information

brand :

R&D Systems

master code :

3239-S3B

SKU :

3239-S3B-025

product name :

Recombinant Human Semaphorin 3E Protein, CF

description :

The Recombinant Human Semaphorin 3E Protein, CF from R&D Systems is derived from NS0. The Recombinant Human Semaphorin 3E Protein, CF has been validated for the following applications: Bioactivity.

target :

Semaphorin 3E

category :

Proteins and Enzymes

sizes available :

25 ug

buffer :

Lyophilized from a 0.2 µm filtered solution in PBS, NaCl and Tween® 20 with Trehalose.

conjugate :

Unconjugated

purity :

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

species :

Human

observed molecular weight :

85-100 kDa, 66 kDa and 25 kDa, reducing conditions

theoretical molecular weight :

88 kDa

gene symbol :

SEMA3E

details of functionality :

Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al . (2010) Circ. Res. 106 :391. The ED50 for this effect is 0.04-0.24 µg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin D1 (Catalog # 4160-PD ) is coated at 5 µg/mL, Recombinant Human Semaphorin 3E binds with an apparent Kd 0.4 nM.

endotoxin note :

<1.0 EU per 1 µg of the protein by the LAL method.

accessionNumbers :

O15041

applications :

Bioactivity

source :

NS0-derived Recombinant Human Semaphorin 3E Protein, CF

USD :

509 USD

alt names :

(semaphorin) 3E, coll-5, KIAA0331M-SEMAH, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, Sema3E, SEMAH, SEMAHM-SemaK, semaphorin-3E

storage :

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

more info or order :

R&D Systems product webpage