This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
R&D Systems
product type :
protein
product name :
Recombinant Human Nodal Protein
catalog :
3218-ND
quantity :
25 ug
price :
389 USD
citations: 15
Reference
Harpelunde Poulsen K, Nielsen J, Grønkær Toft B, Joensen U, Rasmussen L, Blomberg Jensen M, et al. Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours. BMC Cancer. 2020;20:349 pubmed publisher
Asnaghi L, White D, Key N, Choi J, Mahale A, Alkatan H, et al. ACVR1C/SMAD2 signaling promotes invasion and growth in retinoblastoma. Oncogene. 2019;38:2056-2075 pubmed publisher
Ray P, Chin A, Worley K, Fan J, Kaur G, Wu M, et al. Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping. Proc Natl Acad Sci U S A. 2018;115:E11568-E11577 pubmed publisher
Jørgensen A, Macdonald J, Nielsen J, Kilcoyne K, Perlman S, Lundvall L, et al. Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis. Cell Rep. 2018;25:1924-1937.e4 pubmed publisher
Jiang Y, Li H, Wang Y, Tian T, He Y, Jin Y, et al. ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line. Oncol Lett. 2017;14:6955-6961 pubmed publisher
Ball C, Oppel F, Ehrenberg K, Dubash T, Dieter S, Hoffmann C, et al. Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts. EMBO Mol Med. 2017;9:918-932 pubmed publisher
Lee G, Fujita M, Takaoka K, Murakami Y, Fujihara Y, Kanzawa N, et al. A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO. J Cell Biol. 2016;215:705-718 pubmed
Fuerer C, Nostro M, Constam D. Nodal·Gdf1 heterodimers with bound prodomains enable serum-independent nodal signaling and endoderm differentiation. J Biol Chem. 2014;289:17854-71 pubmed publisher
Esquivies L, Blackler A, Peran M, Rodriguez Esteban C, Izpisua Belmonte J, Booker E, et al. Designer nodal/BMP2 chimeras mimic nodal signaling, promote chondrogenesis, and reveal a BMP2-like structure. J Biol Chem. 2014;289:1788-97 pubmed publisher
Quail D, Zhang G, Findlay S, Hess D, Postovit L. Nodal promotes invasive phenotypes via a mitogen-activated protein kinase-dependent pathway. Oncogene. 2014;33:461-73 pubmed publisher
Wilson T, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, et al. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature. 2012;487:505-9 pubmed publisher
Castonguay R, Werner E, Matthews R, Presman E, Mulivor A, Solban N, et al. Soluble endoglin specifically binds bone morphogenetic proteins 9 and 10 via its orphan domain, inhibits blood vessel formation, and suppresses tumor growth. J Biol Chem. 2011;286:30034-46 pubmed publisher
Herrera B, Inman G. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. BMC Cell Biol. 2009;10:20 pubmed publisher
Watanabe K, Hamada S, Bianco C, Mancino M, Nagaoka T, Gonzales M, et al. Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor. J Biol Chem. 2007;282:35772-86 pubmed
McLean A, D Amour K, Jones K, Krishnamoorthy M, Kulik M, Reynolds D, et al. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Stem Cells. 2007;25:29-38 pubmed
product information
brand :
R&D Systems
master code :
3218-ND
SKU :
3218-ND-025
product name :
Recombinant Human Nodal Protein
description :
The Recombinant Human Nodal Protein from R&D Systems is derived from E. coli. The Recombinant Human Nodal Protein has been validated for the following applications: Bioactivity.
target :
Nodal
category :
Proteins and Enzymes
unit size :
25 ug
buffer :
Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
conjugate :
Unconjugated
purity :
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
species :
Human
theoretical molecular weight :
12.9 kDa (monomer)
gene symbol :
NODAL
details of functionality :
Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Yeo, C. et al . (2001) Mol. Cell. 7 :949. Approximately 100 ng/mL of Recombinant Human Nodal can effectively induce Smad2 phosphorylation.
endotoxin note :
<0.10 EU per 1 µg of the protein by the LAL method.
catalog number base :
3218-ND
accessionNumbers :
Q96S42
applications :
Bioactivity
source :
E. coli -derived human Nodal protein His238-Leu347, with an N-terminal Met
2020 USD :
369
2021 USD :
389 USD
alt names :
BMP-16, MGC138230, nodal homolog, nodal homolog (mouse), nodal, mouse, homolog
storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
company information
R&D Systems
614 McKinley Place N.E.
Minneapolis, MN 55413
info@RnDSystems.com
https://www.rndsystems.com
800 343-7475
headquarters: USA
R&D Systems develops and manufactures high-quality proteins and serves as a world leader in immunoassays. R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry.